Toshio Yagyu
Osaka University
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Featured researches published by Toshio Yagyu.
International Journal of Cancer | 1999
Yuichi Kawabata; Naohiro Tomita; Takushi Monden; Masayuki Ohue; Tadashi Ohnishi; Masaya Sasaki; Mitsugu Sekimoto; Isao Sakita; Yasuhiro Tamaki; Juro Takahashi; Toshio Yagyu; Hideyuki Mishima; Nobuteru Kikkawa; Morito Monden
In a series of 45 poorly differentiated adenocarcinomas (por) and 7 signet‐ring‐cell carcinomas (sig) of the colorectum, K‐ras gene mutation, p53 immunostaining and microsatellite instability (MSI) were analyzed for a comparison with 46 cases of colorectal carcinomas of the well or moderately differentiated type (well/mod). In addition, the mutations of simple repeated sequences in the transforming‐growth‐factor‐β type‐II receptor (T β R‐II) gene and the BAX gene were analyzed as possible targets for DNA replication errors. Mutation of the K‐ras gene in the por, sig and well/mod specimens was detected in, respectively, 22%, 11% and 48%, positive immunostaining for p53 in 41.8%, 28.6% and 60.3%, and MSI in 36%, 30% and 4%. Frameshift mutation of the T β R‐II gene was detected in 27.5% of the por and none of the sig specimens, while corresponding figures for mutation of the BAX gene were 15.7% and 0%. Significant differences between the por and well/mod tumors were found in the occurrence of K‐ras mutation at codons 12 and 13, and MSI. Clinicopathologically, the tumor status of por with MSI was found to significantly correlate with the tumors location in the proximal colon. In cases without MSI and sig, no frameshift mutation of either the T β R‐II or the BAX gene was found. These results suggest that poorly differentiated and signet‐ring‐cell carcinomas have a genetic background different from that of well or moderately differentiated carcinomas of the colorectum, and that DNA‐replication error is at least partly involved in the carcinogenesis of these specific types of colorectal carcinomas. Int. J. Cancer (Pred. Oncol.) 84:33–38, 1999.
Cancer | 1992
Takushi Monden; Hideki Morimoto; Takashi Shimano; Toshio Yagyu; Masahiro Murotani; Hirohito Nagaoka; Yasuhito Kawasaki; Tetsuro Kobayashi; Takesada Mori
OK‐432 (5 KE), an immunomodulatory agent prepared from an attenuated strain of Streptococcus pyogenes, was dissolved in 1 ml of aprotinin (1000 KIE) and mixed with 80 mg of fibrinogen containing Factor XIII. A single intratumoral injection of the mixture was performed preoperatively under endoscopy in 20 patients with colorectal carcinoma. Postoperative histopathologic examinations revealed the formation of fibrin fibers at the site of injection and marked infiltration of inflammatory cells into the tumor stroma on the day after injection; the formation of granulomas containing many giant cells after 4 to 7 days; and extensive regression of tumor tissue after 14 days. This study suggests that the high concentration of exogenous fibrinogen gelatinized enough to trap OK‐432 in tumor stroma and that OK‐432 induced granulomatous hypersensitivity to degenerate tumor stroma, thereby causing regression of the tumors.
Cancer Letters | 1996
Isao Miyashiro; Tadashi Kaname; Takahiro Nakayama; Shoji Nakamori; Toshio Yagyu; Takushi Monden; Nobuteru Kikkawa; Isamu Nishisho; Takashi Muramatsu; Morito Monden; Tetsu Akiyama
Midkine (MK) is a growth differentiation factor originally found as the product of a retinoic acid-responsive gene. The expression of MK was examined in 35 surgically resected specimens of primary colorectal cancer using the reverse transcription-polymerase chain reaction (RT-PCR). All of the cancerous tissues expressed MK. In 5/25 cancerous tissues a truncated form of MK, which was recently found in various human tumor cell lines, was detected in addition to the full-size MK. In contrast, the truncated from of MK could not be detected in non-cancerous tissues, whereas the wild-type form was detected in 8/10 non-cancerous tissues. These results suggest that the expression of the truncated form of MK may be associated with tumorigenesis.
Japanese Journal of Cancer Research | 1992
Toshio Yagyu; Takushi Monden; Yasuhiro Tamaki; Hideki Morimoto; Tsutomu Takeda; Tetsuro Kobayashi; Takashi Shimano; Hiroki Murakami; Takesada Mori
Human hybridomas were generated through the fusion of the human B‐lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes of colonic cancer patients who had received a local immunotherapy. A total of 353 hybridomas were obtained from 4 patients and 116 of these were found to secrete ≧ 100 ng/ml human immunoglobulin. The efficiency was remarkably high as compared with that from patients without the local immunotherapy. Further immunohistological examination showed that 5 hybridomas secreted IgM which selectively reacted with colonic cancers. The results indicate that local immunotherapy could be an adjunctive tool for the generation of highly potent human hybridomas through augmenting the hosts immunity.
Biotherapy | 1992
Hirohito Nagaoka; Takushi Monden; Isao Sakita; Yoshihiro Katsumoto; Taro Wakasugi; Yasuhito Kawasaki; Naohiro Tomita; Tsutomu Takeda; Toshio Yagyu; Hideki Morimoto; Tetsuro Kobayashi; Takashi Shimano; Takesada Mori
We have previously reported that the antitumor effect of OK-432, aStreptococcal preparation, is markedly augmented when injected intratumorally together with fibrinogen (Cancer, 69: 636–642, 1992). In order to elucidate the mechanism of the antitumor effects, we established T cell clones from regional lymph nodes of colorectal cancer patients who received this local immunotherapy. By culture of lymph node lymphocytes, in the presence of IL-2 and OK-432, 4 clones of T cells were established from 4 patients treated by local immunotherapy. These clones had a helper T cell phenotype (CD3+, CD4+, CD8−, CD56−, WT31+) and were successfully maintained for several months. The cells strongly expressed CD25 when stimulated with OK-432 and exhibited a high level of cytotoxic activity in part explained by the increased expression of ICAM-1 and LFA-1, and the release of TNFβ. These results suggest that the CD4+ T cells play a role in the antitumor mechanism of local immunotherapy.
Japanese Journal of Cancer Research | 1993
Toshio Yagyu; Takushi Monden; Masashi Baba; Yasuhiro Tamaki; Tsutomu Takeda; Tetsuro Kobayashi; Takashi Shimano; Yoshiyuki Tsuji; Hirohisa Matsushita; Hisao Osawa; Hiroki Murakami; Takesada Mori
A human monoclonal antibody, YJ‐37 (IgM) was generated through the fusion of human B lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes from a colonic cancer patient who was treated with a local immunotherapy. This antibody was purified and conjugated with biotin, after which direct immunohistochemical staining was performed. The results revealed that YJ‐37 selectively reacted with colonic cancer (7/19), gastric cancer (3/6), endometrial cancer (1/2) and colonic adenoma (7/13), but not with normal epithelia. Membrane immunofluorescence and FACS analysis also showed that YJ‐37 bound to tumor cell surfaces. Furthermore, the chemical structure of the antigen defined by YJ‐37 was analyzed by means of thin‐layer chromatography immunostaining and ELISA. The results indicated that YJ‐37 reacted with sialylated lacto‐series carbohydrate chains, which have been reported to accumulate in cancer cells.
Human antibodies | 1993
Masashi Baba; Tetsuro Kobayashi; Yoshio Oka; Toshio Yagyu; Yasuhiro Tamaki; Tsutomu Takeda; Takushi Monden; Takashi Shimano; Yoshiyuki Tsuji; Hiroki Murakami; Takesada Mori
An anaplastic carcinoma cell line, KOA-2, was established from a 59-year-old patient with anaplastic carcinoma of the thyroid who died only one month after diagnosis in association with rapid progress of her disease. A human monoclonal antibody, 3C5 (IgM), was generated by a fusion of human B lymphoblastoid cell line, HO-323, with lymphocytes from cervical lymph nodes of the patient, who had been treated with local immunotherapy. Immunocytostaining demonstrated that 3C5 reacted with KOA-2, and membrane immunofluorescence demonstrated that 3C5 reacted with cell membranes. Immunohistochemical staining studies demonstrated selective reaction of 3C5 with three malignant tumors, anaplastic carcinoma of the thyroid (2/4 sampled tested), papillary carcinoma of the thyroid (8/12), and breast cancer (2/6), but no reaction with specimens of benign thyroid disease or normal thyroid gland was demonstrated. The monoclonal antibody and the cell line established from one patient have potential use in the analysis of carcinogenesis and applications to therapy of anaplastic carcinoma of the thyroid.
Acta Histochemica Et Cytochemica | 1990
Nobuhiko Haruna; Takushi Monden; Hideki Morimoto; Masahiro Murotani; Toshio Yagyu; Hirohito Nagaoka; Takashi Shimano; Takesada Mori
Hybridoma | 1992
Masashi Baba; Tetsuro Kobayashi; Yasuhiro Tamaki; Hideyuki Mishima; Toshio Yagyu; Hideki Morimoto; Takushi Monden; Takashi Shimano; Yoshiyuki Tsuji; Hiroki Murakami; Takesada Mori
The Japanese Journal of Gastroenterological Surgery | 2002
Mariko Man-i; Isamu Nishisho; Hideyuki Mishima; Toshio Yagyu; Nobuteru Kikkawa; Toshimasa Tsujinaka