V. Remya

Repair of abdominal wall hernias using acellular dermal matrix in goats

The present study was undertaken to evaluate the efficacy of acellular dermal matrix (ADM) of caprine origin for the repair of abdominal wall hernias in six goats. The skin of goat was de-epithelialised using a 2 M sodium chloride solution and 0.25% trypsin, and further decellularised using 2% sodium deoxycholate, which was then used as graft material. Under xylazine sedation and local infiltration analgesia, the abdominal wall hernias were repaired with ADM graft using inlay graft technique. All animals had an uneventful recovery without clinical signs of wound dehiscence, infection or recurrence of hernia during 6 months follow-up periods.

Polypropylene mesh seeded with fibroblasts: A new approach for the repair of abdominal wall defects in rats

PURPOSE The purpose of study was to develop bioengineered scaffolds by seeding primary mouse embryo fibroblast cells (p-MEF) on polypropylene mesh and to test its efficacy for the repair of abdominal wall defects in rats. METHODS The study was conducted on 18 clinically healthy adult Wistar rats of either sex. The animals were randomly divided into two equal groups having nine animals in each group. In both the groups a 20mm×20mm size full thickness muscle defect was created under xylazine and ketamine anesthesia in the mid-ventral abdominal wall. In group I the defect was repaired with polypropylene mesh alone and in group II it was repaired with p-MEF seeded polypropylene mesh. Matrices were implanted by synthetic absorbable suture material (polyglycolic acid) in continuous suture pattern. The efficacy of the bio-engineered matrices in the reconstruction of full thickness abdominal wall defects was evaluated on the basis of macro and histopathological observations. RESULTS Macroscopic observations revealed that adhesions with skin and abdominal viscera were minimum in group II as compared to group I. Histopathological observations confirmed better fibroplasia and collagen fiber arrangement in group II. No recurrence of hernia was found in both the groups. CONCLUSION Hernias are effectively repaired by implanting polypropylene mesh. However, this work demonstrates that in vitro seeding of mesh with fibroblasts resulted in earlier subsidization of pain, angiogenesis and deposition of collagen, increased thickness of matrices with lesser adhesions with underlying viscera. On the basis of the results p-MEF seeded mesh was better than non-seeded mesh for repair of abdominal wall defects in rats.