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Dive into the research topics where Vanusia Batista de Oliveira Amorim is active.

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Featured researches published by Vanusia Batista de Oliveira Amorim.


Genetics and Molecular Biology | 2009

Genetic diversity of carotenoid-rich bananas evaluated by Diversity Arrays Technology (DArT)

Edson Perito Amorim; Alberto Duarte Vilarinhos; Kelly de Oliveira Cohen; Vanusia Batista de Oliveira Amorim; Janay Almeida dos Santos-Serejo; Sebastião de Oliveira e Silva; Kátia N. Pestana; Vânia J. dos Santos; N. S. Paes; Damares C. Monte; Ronaldo V. dos Reis

The aim of this work was to evaluate the carotenoid content and genetic variability of banana accessions from the Musa germplasm collection held at Embrapa Cassava and Tropical Fruits, Brazil. Forty-two samples were analyzed, including 21 diploids, 19 triploids and two tetraploids. The carotenoid content was analyzed spectrophotometrically and genetic variability was estimated using 653 DArT markers. The average carotenoid content was 4.73 μg.g -1 , and ranged from 1.06 μg.g -1 for the triploid Nanica (Cavendish group) to 19.24 μg.g -1 for the triploid Saney. The diploids Modok Gier and NBA-14 and the triploid Saney had a carotenoid content that was, respectively, 7-fold, 6-fold and 9-fold greater than that of cultivars from the Cavendish group (2.19 μg.g -1). The mean similarity among the 42 accessions was 0.63 (range: 0.24 to 1.00). DArT analysis revealed extensive genetic variability in accessions from the Embrapa Musa germplasm bank.


Plant Molecular Biology Reporter | 2010

Polymorphism of Microsatellite Markers in Papaya (Carica papaya L.)

Eder Jorge de Oliveira; Vanusia Batista de Oliveira Amorim; Edneide Luciana Santiago Matos; Juliana Leles Costa; Milene da Silva Castellen; Juliano Gomes Pádua; Jorge Luiz Loyola Dantas

A set of 81 new microsatellite markers for Carica papaya L. previously identified by data mining using freely available sequence information from Genbank were tested for polymorphism using 30 germplasm accessions from the Papaya Germplasm Bank (PGM) at Embrapa Mandioca e Fruticultura Tropical (CNPMF) and 18 landraces. The data were used to estimate pairwise genetic distances between the genotypes. A neighbor-joining based dendrogram was used to define clusters and infer possible genetic structuring of the collection. Most microsatellites were polymorphic (73%), with an observed number of alleles per locus ranging from one to eleven. The levels of observed and expected heterozygosity for 51 polymorphic loci varied from 0.00 to 0.85 and from 0.08 to 0.82, averaging 0.19 and 0.59, respectively. Forty-four percent of microsatellites showed polymorphism information content (PIC) higher than 0.50. The compound microsatellites seem to be more informative than dinucleotide and trinucleotide repeats in average alleles per locus and PIC. Among dinucleotides, AG/TC or GA/CT repeat motifs exhibited more informativeness than TA/AT, GT/CA and TG/AC repeat motifs. The neighbor-joining analysis based on shared allele distance could differentiate all the papaya accessions and landraces as well as differences in their genetic structure. This set of markers will be useful for examining parentage, inbreeding and population structure in papaya.


Euphytica | 2011

Agronomic and molecular characterization of gamma ray induced banana (Musa sp.) mutants using a multivariate statistical algorithm

Rosa Karla Nogueira Pestanana; Edson Perito Amorim; Claudia Fortes Ferreira; Vanusia Batista de Oliveira Amorim; Larissa Santos Oliveira; Carlos Alberto da Silva Ledo; Sebastião de Oliveira e Silva

Bananas are tropical fruits grown worldwide playing a key role in market trade and especially used as main food source for low income populations. In Brazil, bananas are mainly consumed in natura, occupying the second largest internal market. Nevertheless, this crop presents low availability of productive commercial varieties with good agronomic characteristics. A strategy undertaken to solve this problem is the development of new cultivars through conventional genetic breeding methods. However, this strategy presents some obstacles such as female sterility and low number of seeds. In order to overcome these shortcomings, use of mutation induction aiming the selection of mutants with desirable agronomic characteristics seems to have great potential for developing new cultivars. The objective of the present work was to evaluate the genetic variability in putative banana ‘Pacovan’ (AAB genome, subgroup Prata Type) mutants submitted to gamma ray irradiation, using a set of agronomical and molecular data (ISSR markers). The distance between the putative ‘Pacovan’ mutants varied from 0.26 to 0.64 with cophenetic correlation coefficient of 0.7669. Four mutants were selected based on best agronomical characteristics and height. This data also shows that there is variability that can be explored after the irradiation of ‘Pacovan’ banana mutants, which can be used in the genetic breeding program of banana aiming to develop short new varieties that also present good agronomic characteristics. This is the first attempt to use combined data in order to evaluate the genetic variability in putative banana mutants.


Pesquisa Agropecuaria Brasileira | 2008

Variabilidade genética estimada entre diplóides de banana por meio de marcadores microssatélites

Edson Perito Amorim; Ronaldo Viana dos Reis; Janay Almeida dos Santos-Serejo; Vanusia Batista de Oliveira Amorim; Sebastião de Oliveira e Silva

O objetivo deste trabalho foi estimar a divergencia genetica entre 38 diploides de banana do programa de melhoramento da Embrapa Mandioca e Fruticultura Tropical, incluindo genotipos melhorados, cultivados e selvagens, por meio de 15 marcadores microssatelites ou SSR. As similaridades geneticas, com base no coeficiente de Jaccard, foram utilizadas para fazer o agrupamento dos genotipos pelo metodo UPGMA. O numero de alelos obtidos foi 113, com media de 7,53 alelos por iniciador. A similaridade genetica media foi de 0,22, e variou de 0,028 a 0,48, o que indica existencia de variabilidade genetica entre os genotipos. A analise de grupos, com base no polimorfismo de microssatelites, nao pode separar completamente os hibridos melhorados, cultivados e selvagens. Alguns diploides agruparam-se com base em sua origem geografica, entre eles Musa ornata e IAC-1, e Tjau Lagada e Lidi, enquanto que, em outros, nenhuma relacao foi estabelecida. Houve tendencia de agrupamento entre os diploides aparentados, como: SH3263 e 8694-20, 4279-01 e 9179-03, 1304-06 e 5854-03, 86B79-10 e 7341-03, 86B79-12 e 0337-02, e 9194-04 e 4154-08.


Food Chemistry | 2014

Characterisation of metabolic profile of banana genotypes, aiming at biofortified Musa spp. cultivars

Cristine Vanz Borges; Vanusia Batista de Oliveira Amorim; Fernanda Ramlov; Carlos Alberto da Silva Ledo; Marcela Donato; Marcelo Maraschin; Edson Perito Amorim

The banana is an important, widely consumed fruit, especially in areas of rampant undernutrition. Twenty-nine samples were analysed, including 9 diploids, 13 triploids and 7 tetraploids, in the Active Germplasm Bank, at Embrapa Cassava & Fruits, to evaluate the bioactive compounds. The results of this study reveal the presence of a diversity of bioactive compounds, e.g., catechins; they are phenolic compounds with high antioxidant potential and antitumour activity. In addition, accessions with appreciable amounts of pVACs were identified, especially compared with the main cultivars that are currently marketed. The ATR-FTIR, combined with principal components analysis, identified accessions with distinct metabolic profiles in the fingerprint regions of compounds important for human health. Likewise, starch fraction characterisation allowed discrimination of accessions according to their physical, chemical, and functional properties. The results of this study demonstrate that the banana has functional characteristics endowing it with the potential to promote human health.


Euphytica | 2010

Polymorphic microsatellite marker set for Carica papaya L. and its use in molecular-assisted selection.

Eder Jorge de Oliveira; Aline dos Santos Silva; Fabiana Moraes de Carvalho; Lucas Ferraz dos Santos; Juliana Leles Costa; Vanusia Batista de Oliveira Amorim; Jorge Luiz Loyola Dantas


Pesquisa Agropecuaria Brasileira | 2010

Agronomical and molecular characterization of banana germplasm

Lorenna Alves Mattos; Edson Perito Amorim; Vanusia Batista de Oliveira Amorim; Kelly de Oliveira Cohen; Carlos Alberto da Silva Ledo; Sebastião de Oliveira e Silva


Acta Horticulturae | 2013

BANANA BREEDING AT EMBRAPA CASSAVA AND FRUITS

Edson Perito Amorim; J.A. dos Santos-Serejo; Vanusia Batista de Oliveira Amorim; Claudia Fortes Ferreira; Sebastião de Oliveira e Silva


Plant Cell Tissue and Organ Culture | 2016

Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes

F. V. D. Souza; Ergun Kaya; Lívia de Jesus Vieira; Everton Hilo de Souza; Vanusia Batista de Oliveira Amorim; Dianne Skogerboe; Tracie K. Matsumoto; Alfredo Augusto Cunha Alves; Carlos Alberto da Silva Ledo; Maria M. Jenderek


Archive | 2016

Diversidade genética de espécies do vírus das estrias da bananeira (BSV) na coleção de germoplasma da Embrapa Mandioca e Fruticultura.

Dalma Brito; Camila Pestana; Kátia Nogueira Pestana; Vanusia Batista de Oliveira Amorim; Marie-Line Iskra Caruana; Matthieu Chabannes; Edson Perito Amorim; Claudia Fortes Ferreira

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Edson Perito Amorim

Empresa Brasileira de Pesquisa Agropecuária

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Sebastião de Oliveira e Silva

Empresa Brasileira de Pesquisa Agropecuária

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Carlos Alberto da Silva Ledo

Empresa Brasileira de Pesquisa Agropecuária

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Claudia Fortes Ferreira

Empresa Brasileira de Pesquisa Agropecuária

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Janay Almeida dos Santos-Serejo

Empresa Brasileira de Pesquisa Agropecuária

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Eder Jorge de Oliveira

Empresa Brasileira de Pesquisa Agropecuária

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Jorge Luiz Loyola Dantas

Empresa Brasileira de Pesquisa Agropecuária

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Juliana Leles Costa

Universidade Federal do Recôncavo da Bahia

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Kelly de Oliveira Cohen

Empresa Brasileira de Pesquisa Agropecuária

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Ronaldo Viana dos Reis

Universidade Federal de Viçosa

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