Vera Hadacova
Czechoslovak Academy of Sciences
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Biologia Plantarum | 1983
Eva Klozová; Jiřina Švachulová; J. Smartt; Emil Hadač; Věra Turková; Vera Hadacova
The seed protein patterns of 12Arachis species were compared by polyacrylamide gel electrophoresis (PAGE), similarities between patterns were measured by the Jaccard index. Results obtained confirm the close relationships established between members of the genus on morphological grounds and support the more recent classification schemes.A. villosa andA. correntina could well be regarded as distinct species on grounds of protein differences whileA. macedoi andA. villosulicarpa (although members of the same section, Extranervosae) show considerable differentiation of their protein patterns. Surprisingly, the formA. ×batizogaea showed less similarity in protein pattern to those of its parental species than might have been expected. The principle value of seed protein pattern data appears to be in distinguishing species within sections.
Biologia Plantarum | 1981
Eva Klozová; Vera Turkova; Vera Hadacova; Jiřina Švachulová
Similarity of seed protein spectra of 13 representatives of the genusAllium, subgenusRhizirideum, was estimated by means of immunochemical analysis; the species investigated were divided into four groups more or less corresponding to the contemporary systematic division of species into sections.
Biologia Plantarum | 1983
Vera Hadacova; Květa Vacková; Eva Klozová; M. Kutáček; Květa Pitterová
In partly purified protein complexes obtained from 22 species of theAllium genus and 6 cultivars ofAllium cepa the activity of cholinesterases was detected and measured using the method of Ellman et al. The degree of its inhibition with 10-4 M neostigmine was also tested. It was found that the activity of cholinesterase differed in individual species up to two hundred times, while the differences in the inhibitory activity of 10-4 M neostigmine occurred only in a few cases. Individual sections and cultivars could not be characterized on the basis of the differences in the activities of the cholinesterases. Of all the sections that ofPhyllodolon shows the highest average activity. In the case of the tested cultivars distinctly the lowest activity was observed in cv. Kastická. The values of the enzymatic activity measured by Ellman’s method in this plant material include the activity of specific and unspecific cholinesterases and the part uninhibitable by neostigmine.
Biologia Plantarum | 1983
Vera Hadacova; Eva Klozová; Jiŕina Svachulová; Kvéta Pitterová
Malate dehydrogenase isoenzymes were studied in tenAllium species and in six cultivars ofA. cepa by isoelectric focusing in polyacrylamide gel with Ampholine pH 3.5–10.0. Using this method better resolution was obtained than by polyacrylamide gel electrophoresis. The number of MDH isoenzymes obtained by isoelectric focusing is from five to ten in the range of pH 3.65 to 6.75. MDH isoenzymes can be used for characterization on the level of species and cultivars (inA. cepa), but its use on the level of sections and subgenera is questionable.
Biologia Plantarum | 1982
Karel Beneš; Vera Hadacova
Using polyacryl amide gel electrophoresis isoenzyme patterns were studied of α- and β-glucosidase, α- and β-galactosidase, α-fucosidase, β-glucosaminidase, β-glucuronidase, α-man-nosidase and β-xylosidase in whole root tips, in particular growth zones and in the cortex and central cylinder of broad bean and maize. Though considerable differences even in number and position of isoenzymes were found in particular cases in both objects, no general conclusion could be reached. The mentioned differences are not obligatory and have been observed both between zones of division and elongation and between zones of elongation and maturation. This fact is hard to understand on the basis of proteosynthetic processes involved in proliferative and postproliferative cell growth.
Folia Geobotanica | 1969
Emil Hadač; Vera Hadacova
Biologia Plantarum | 1975
Vera Hadacova; M. Kamínek; J. Luštinec
Biologia Plantarum | 1974
Vera Hadacova
Biologia Plantarum | 1972
Vera Hadacova
Biologia Plantarum | 1969
Vera Hadacova; Miroslav Dvorak