Vernon P. Perry

Long-term storage of bone marrow cells at liquid nitrogen and dry ice temperatures☆

Abstract Bone marrow frozen with glycerol and stored at −69 to 79 °C for 3 to 9 years was compared with bone marrow frozen with DMSO and stored for 3 years in liquid nitrogen (−120 to −196 °C). It was found that bone marrow cells stored in Dry Ice were almost entirely destroyed after 3 years of storage, while bone marrow cells in liquid nitrogen were largely intact after 3-year storage.

FURTHER OBSERVATIONS ON THE COLLECTION, STORAGE AND TRANSFUSION OF PERIPHERAL BLOOD LEUKOCYTES*

The peripheral blood of 15 guinea pigs was collected over a six-month period and processed by density differential centrifugation yielding a total leucocyte number of from 5.6 to 8.7 x 10⁷ celis. The nucleated cells were frozen and stored in iiquid nitrogen for periods ranging from 31 to 245 days and subsequently reinfused as a single inoculation into lethally irradiated donor animals. Four animais survived beyond the period of maximum survival of the control animals. It is suggested that inadequate cell numbers were employed in this study, and that perhaps larger numbers of cells infused over a longer period of time will prove to be more effective in protecting against the effects of lethal radiation. (auth)

Nutrient medium and frozen storage of human lymphocytes

Abstract 1. 1. Lymphocytes to be used for tissue typing procedures can be stored at room temperature in a nutrient medium for periods of up to 3 wk without an intervening change of fluid medium. 2. 2. Fresh or nutrient medium stored lymphocytes can be frozen in bulk quantities until they are required for tissue typing studies, and/or 3. 3. Depending upon the facilities of the laboratory (1) fresh, (2) nutrient medium stored, and (3) frozen and thawed cells can be distributed into 72-well tissue typing trays, frozen for either short or long term studies under controlled rate procedures and stored in the vapor phase of liquid nitrogen for future typing studies.

Nutrient media storage of human bone marrow.

Summary The procedure employed for the short-term nutrient media storage of autologous bone marrow is presented. Data obtained from 26 cases of nutrient media storage of an experimental series of bone marrow obtained from elective operative procedures are also included.

Freeze drying of histocompatibility typing sera.

Abstract This report describes a unit employed for the freeze drying of histocompatibility typing serum using a 50-hr cycle. This unit will process approximately 3200 3-ml vials with a final residual moisture content of less than 2%. The system employs dry ice-alcohol cooled circulating baths to maintain the condensers below −60 °C, and two shelf cooling baths to maintain the product at required temperatures during the freeze drying process. The results of a 5-yr study of the effect of residual moisture as a function of time and storage temperature is also included. Studies conducted to date indicate that with residual moistures below 2%, freeze dried histocompatibility sera can be stored at +4 °C without the loss of significant tissue typing factors. Solubility of all serum was lost when stored at +37 °C or higher during this same 5-year period.