Victor O. Adetimirin

Population structure and molecular characterization of nigerian field genebank collections of cacao, Theobroma cacao L.

Abstract Inadequate knowledge of the population structure and diversity present often hamper the efficient use of germplasm collections. Using a high through-put system, twelve microsatellite loci were used to analyze genetic diversity and population structure in a national field genebank repository of 243 cacao accessions grouped into 11 populations based on their known sources. Based on multi-locus profiles, the Bayesian method was used for individual assignment to verify membership in each population, determine mislabeling and ancestry of some important accessions used in breeding program. A total of 218 alleles was revealed with a mean number of 18.2 alleles per locus. Gene diversity (He = 0.70) and allelic richness (4.34 alleles per locus) were highest in the F1 hybrid population. Differential mating system was suggested as responsible for the observed deficit and excess of heterozygotes observed among the populations. Analysis of molecular variance showed that within-population variance accounted for 63.0% of the total variance while the rest 37% was accounted for by the among-population variance. Cluster dendrogram based on UPGMA revealed two main subsets. The first group was made up of the Amelonado/Trinitario ancestry and the other of Nanay/Parinari ancestry. We found that Nanay and Parinari populations were the major source of Upper Amazon genes utilized while a large proportion of genetic diversity in the field genebank remained under-utilized in development of improved cultivars released to farmers in Nigeria. This study showed that the presence of alleles of the Upper Amazon Forasteros (Nanay, Parinari and Iquitos Mixed Calabacillo) genetic materials in the locally available accessions predated the formal large scale introduction of Upper Amazon materials in 1944. This is the first report of population structure of field genebank collections of cacao in Nigeria since more than seven decades of formal cacao breeding research.

Conditioning effects of Striga hermonthica seed on field performance of maize

Abstract Seeds of the obligate parasite Striga hermonthica , germinate in response to stimulants in the root exudate of maize and other host crops. Under laboratory conditions, conditioning (defined as treatment with moisture) of Striga seeds for one to three weeks before exposure to germination stimulant is required for optimum germination. The objective of this study was to determine if maize performance and Striga emergence, both indices of resistance, were influenced by the interval between Striga seed infestation and maize planting, under high parasite inoculum (3000–6000 germinable seeds) as practiced in maize resistance breeding field nurseries. Four conditioning treatments were investigated: maize planted immediately after Striga seed infestation, and maize planted after one, two and three weeks after infestation. Experimental design was randomized complete block with four replications. Differences among the four conditioning treatments were not significant for maize damage score, height, stalk lodging, number of ears harvested, grain yield and number of emerged Striga plants. Thus, maize planted on the same day as Striga infestation showed similar response as maize planted one to three weeks after infestation. Using high seed inoculum, maize can be planted immediately after Striga infestation in maize breeding nurseries. Combining Striga seed infestation and maize planting into a single field operation is convenient and reduces cost of screening.

Genetic Variation for Nitrogen-Use Efficiency Among Selected Tropical Maize Hybrids Differing in Grain Yield Potential

Low soil nitrogen (N) and sub-optimal N fertilizer applications result in poor grain yield (GY) in maize. Genotypes with improved N-use efficiency (NUE) are particularly beneficial to low-input agriculture. Information on the relative importance of the main components of NUE will facilitate genetic improvement of tropical maize for NUE. This study evaluated genetic variation for NUE among tropical maize hybrids selected for contrasting responses to N. The hybrids were grown in replicated trials from 2006 to 2008 where plots received either no (0 kg N/ha), low (30 kg N/ha), or high (90 kg N/ha) levels of supplemental N. The results documented significant genetic variation for GY and measured NUE component traits among the hybrids, as well as significant interactions between hybrid and N level for all traits except nitrogen harvest index. Under low N, NUE, NUPE, and NUTE increased by 61%, 21%, and 42%, respectively. Grain yield was significantly and positively correlated with NUE, NUPE, and NUTE at both low N and high N. Both NUPE and NUTE were significantly and positively correlated to NUE. Five hybrids (4001/4008, KU1409/4008, KU1409/9613, 4008/1808, and 1824/9432) produced similar GY at both low N and high N, but differed in their NUPE and NUTE. Genetic variation was present among the maize hybrids studied for NUE and its components. Although both NUPE and NUTE contributed to high grain yield, the relative importance of the two traits to NUE varied with genotype and level of N. This study was part of the major authors PhD thesis, funded by the NSF grant from the University of Illinois at Urbana Champaign and the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. We express our gratitude to Julian Seebauer of Fred Belows Crop Physiology Laboratory, Department of Crop Sciences, University of Illinois at Urbana Champaign for the N analyses and the staff of IITA Ibadan Maize Improvement Program for field assistance.