W. M. Wan Aida

Characterisation of fucoidan extracted from Malaysian Sargassum binderi

Fucoidan is a sulphated polysaccharide that consists mainly of fucose, normally found in brown seaweeds. In this study, fucoidan was extracted from Sargassum binderi (Fsar) from Malaysia and subsequently characterised. The chemical characteristics of Fsar were found to be different than those of commercial food grade fucoidan (Fysk) and those of previously studied fucoidans. NMR analysis proposed that the main structure of Fsar is →3)fuc-2-OSO3(-)(1→3)fuc(1→. The molecular weight (47.87kDa) and degree of sulphation (0.20) of Fsar were higher than those of Fysk, at 27.98kDa and 0.15, respectively. However, Fsars polydispersity index (1.12) and fucose content (34.50%) were lower than those of Fysk, at 1.88 and 43.30%, respectively. Both Fsar and Fysk showed similar thermo-gravimetric properties with four mass losses, amorphous in nature and negative optical rotations. Results show that Fsar has fundamental characteristics of fucoidan with different structural conformation i.e. variation in glycosidic linkages and sulphate group orientation.

Thermal Analysis and Surface Morphology Study of Cholesterol: Β-Cyclodextrin Inclusion Complex

nclusion complex of cholesterol:β-cyclodextrin (CβIC) was characterized for thermal analysis by differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). The difference of endothermic peak between inclusion compounds of cholesterol:β-cyclodextrin (CβIC) with pure cholesterol and β-cyclodextrin endothermic peaks confirmed the formation of inclusion complex. SEM results showed that of cholesterol:β-cyclodextrin inclusion complex (CβIC) observed in the form of irregular crystalline particles which has different morphology from CHO and βCD. The results obtained from this study will able to give valuable information about cholesterol-reduced egg yolk properties that could be useful to produce healthy-egg yolk based products.

Extraction of Sulfated Polysaccharides (Fucoidan) From Brown Seaweed

Abstract Fucoidan is a type of sulfated water-soluble polysaccharide extracted from brown seaweeds. It exhibits strong biological activities, including, antibacterial, antiviral, antitumor, anticoagulant, and antioxidant activities. This sulfated polysaccharide has very diverse chemical structures and compositions, which vary significantly depending on the geographical location, species, seasons, and population age. The varied research interests in fucoidan dictates different extraction procedures of fucoidan, where aqueous systems are favored due to the water solubility of fucoidan. In general, the main concern of the extraction is the yield and purity of the extracted fucoidan, where different extraction methods give different precedence on yield and purity. The selection of suitable fucoidan extraction methods depends on the requirement of the analysis, as well as the intended use of the extracted/isolated fucoidan.

Structural elucidation of fucoidan from Cladosiphon okamuranus (Okinawa mozuku)

Fucoidan is a sulphated polysaccharide, made up mainly of l-fucose, which is found in brown seaweeds. Its chemical structure is diverse and depends on maturity, species and geographical location. The objective of this study was to elucidate the chemical structure of fucoidan from Cladosiphon okamuranus harvested in Japan. The fucoidan was subject to purification prior to monosaccharide profiling, sulphate content determination, and linkage analysis. Our results showed that Japanese Cladosiphon okamuranus fucoidan contained 70.13 ± 0.22 wt% fucose and 15.16 ± 1.17 wt% sulphate. Other minor monosaccharides found were d-xylose, d-galactose, d-mannose, d-glucose, d-arabinose, d-rhamnose and d-glucuronic acid. Linkage analysis revealed that fucopyranoside units along the backbone are linked, through α-1,3-glycosidic bonds, with fucose branching at C-2, and one sulphate group at C-4 per every three fucose units, i.e. the structure of fucoidan from Japanese Cladosiphon okamuranus is [→3)-α-fuc(1→]0.52[→3)-α-fuc-4-OSO3-(1→]0.33[→2)-α-fuc]0.14.