Waleska de Melo Ferreira Dantas

Hemogasometria em eqüinos com compactação experimental do cólon maior tratados com sene, fluidoterapia enteral e parenteral

The blood acid-base status was studied in equines when induced large colon impaction was followed by applying an isotonic polionic enteral solution, or sene associated with intravenous fluid therapy, or fluid therapy alone. The blood samples were collected in twenty animals, four groups (E8, RL, SE, and C) of five animals each. Animals of the treated groups had experimentally induced colon impactions. Equines in group E8 received isotonic polionic enteral solution (8 mL kg -1 h -1 48

Tratamentos da compactação experimental do cólon maior de equinos com hidratação enteral, intravenosa e sene (Cassia augustifolia Vahl)

Treatment of experimental large colon impaction in horses: enteral and parenteral fluid therapy and senna (Cassia augustifolia Vahl) The objective of this study was to investigate the effect of senna associated with intravenous fluid therapy (SEN), isotonic polionic enteral solution (PE) and intravenous fluid therapy (IV) alone in the treatment of experimental large colon impaction in horses. Fifteen horses with experimentally induced colon impaction were divided in three groups: Group PE received the isotonic polionic enteral solution (8 mL kg -1 h -1 , during 48 h); Group SEN received senna (20 mg kg -1 , two doses with 24 h interval) + Ringer lactate i.v . (10 mL kg -1 h -1 12 h -1 , for two days); and Group IV received Ringer lactate i.v. (16 mL kg -1 h -1 12 h -1 , for two days). The treatment protocol of group PE increased fecal hydration and helped remove impaction faster; however, prolonged intestinal hypomotility were observed. The treatment protocol SEN normalized the intestinal motility faster; however the time required to remove impaction was higher and caused higher increase in abdominal distention and pain, as well as less capacity to revert dehydration. The treatment used for group

Hemogasometria e ânion gap em equinos tratados com soluções eletrolíticas enterais contendo diferentes fontes de energia

The present study compared the effects of electrolyte solutions containing different sources of energy that were administrated through enteral route by naso-esophageal probe of small-caliber with continuous flow on the acid base balance in horses. Six adult females were used in two simultaneous 6x3 latin squares mixed model. The animals were divided into three groups and received the following treatments: SEDext - 5g of sodium chloride, 0.5g of potassium chloride, 0.2g of magnesium pidolate, 1g of calcium gluconate and 10g of dextrose diluted in 1.000mL of water. The osmolality measured was of 228mOsmol L-1; SEMalt - 5g of sodium chloride, 0.5g of potassium chloride, 0.2g of magnesium pidolate, 1g of calcium gluconate and 10g of maltodextrin diluted in 1.000mL of water. The osmolality measured was of 181mOsmol L-1 and SEProp - 5g of sodium chloride, 0.5g of potassium chloride, 0.2g of magnesium pidolate, 1g of calcium gluconate and 10g of calcium propionate diluted in 1.000mL of water. The osmolality measured was of 282mOsm L-1. The electrolyte solutions were administered in a dose of 15mL kg-1 h-1 for 12 hours. The treatments with enteral electrolyte solutions containing dextrose, maltodextrin and calcium propionate did not change blood gas analysis values in these animals.

Hemogasometria em cães com desidratação experimental tratados com soluções eletrolíticas comerciais administradas por via intravenosa

Three commercial intravenous electrolyte solutions were compared as for their effects on the blood acid-base status in dogs experimentally dehydrated by withholding water and inducing polyuria. Animals were randomly divided into three groups which were rehydrated with the following commercial electrolyte solutions during 12 hours: Lactate Ringer´s solution (RL), Ringer´s solution (RS) and a normal saline solution (0.9% sodium chloride) containing 5% dextrose (GF). The RL´s intravenous fluid therapy resulted in an alkalinizing effect demonstrated by a mild increase in arterial blood pH, ctCO2, bicarbonate (cHCO-3), and arterial blood base concentration (cBase) and, thus, can be used in animals exhibiting mild to moderate metabolic acidosis. In contrast, the RS and GF therapies led to a mild decrease in the concentration of arterial blood tritiable base (cBase) inducing an acidifying effect, which make them an option to treat dogs with metabolic alkalosis.

Concentração de anticoagulante, tempo e temperatura de armazenagem sobre os parâmetros hematológicos no hemograma automatizado

The study aimed to identify the effects of time, temperature of storage and excess of anticoagulant on hematological parameters of dogs. Blood samples of ten, clinically healthy dogs, of different breeds were utilized. Aliquots were stored with 1.8mg, 3.6mg, 7.2mg and 14.4mg of ethylenediaminetetraacetic acid (EDTA) per mL of blood, divided into two groups: 2°C to 8°C and room temperature. Right after collection, they were evaluated in four times: 0h, 12h, 24h and 48h. White blood cells, red blood cells, hemoglobin, packed cell volume (PCV), mean corpuscular volume (MCV), red cell distribution width (RDW), platelet and thrombocrit (PCT) were evaluated in the automatic cell counter. In the automatic cell counter analysis, the MCV increased significantly with higher concentrations of EDTA (7.2mg mL-1 and 14.4mg mL-1) peaking at 2.36%, in the highest concentration. Temperature and time also influenced the MCV, which showed a decrease in time 12 under temperature of 2°C to 8°C and an increase in time 24 and 48 at room temperature. Storage temperature influenced leukocytes and erythrocytes counts, which showed lower values at room temperature. Hemoglobin, PCV, platelet and PCT did not change significantly. The changes observed did not compromise the results obtained by automatic cell counter, showing that blood samples stored for two days remained in good condition for processing, especially when stored under refrigeration.

Cortisol sérico de equinos com compactação experimental no cólon maior tratados com hidratações enteral e parenteral

O cortisol serico foi estudado em equinos com compactacao experimental do colon maior tratados com fluidoterapias enteral (EN) e intravenosa (IV). Utilizaram-se 10 animais separados em dois grupos de cinco cada. Os animais eram portadores de compactacao induzida no colon maior. O grupo EN foi tratado com solucao isotonica polionica enteral (8 mL/kg/h/48 h), enquanto o grupo IV recebeu Ringer lactato (16 mL/kg/h/12 h, durante dois dias). A compactacao do colon maior ocasionou aumento (P < 0,05) nos valores do cortisol nos animais de ambos os grupos. As solucoes utilizadas no tratamento da compactacao, fluidoterapia enteral com solucao isotonica polionica (EN) e fluidoterapia intravenosa com solucao de Ringer lactato (IV), foram eficientes na reducao dos valores do cortisol serico em equinos com compactacao induzida no colon maior.

Clinical and laboratory assessment of Mangalarga Marchador horses submitted to marcha exercise

José Dantas Ribeiro Filho1*, Rinaldo Batista Viana2, Hélio Cordeiro Manso Filho3, Waleska de Melo Ferreira Dantas4, Micheline Ozana da Silva5, Pedro Ancelmo Nunes Ermita4, Samuel Rodrigues Alves6, Lorena Chaves Monteiro6 & Caio Monteiro Costa6 1 Veterinarian, DSc, Professor. Departamento de Veterinária – DVT, Centro de Ciências Biológicas e da Saúde – CCB, Universidade Federal de Viçosa – UFV, Viçosa, MG, Brasil 2 Veterinarian, DSc, Professor. Instituto da Saúde e Produção Animal ISPA, Universidade Federal Rural da Amazônia UFRA, Belém, PA, Brasil 3 Veterinarian, PhD, Professor. Departamento de ZootecniaDZO, Núcleo de Pesquisa Equina NPE, Universidade Federal Rural do Pernambuco, Recife, PE, Brasil 4 Veterinarians, DSc. Departamento de Veterinária – DVT, Centro de Ciências Biológicas e da Saúde – CCB, Universidade Federal de Viçosa – UFV, Viçosa, MG, Brasil 5 Physiotherapist, DSc. Departamento de Veterinária – DVT, Centro de Ciências Biológicas e da Saúde – CCB, Universidade Federal de Viçosa – UFV, Viçosa, MG, Brasil 6 Veterinarians, MSc. Departamento de Veterinária – DVT, Centro de Ciências Biológicas e da Saúde – CCB, Universidade Federal de Viçosa – UFV, Viçosa, MG, Brasil How to cite: Ribeiro Filho, J. D., Viana, R. B., Manso Filho, H. C., Dantas, W. M. F., Silva, M. O., Ermita, P. A. N., Alves, S. R., Monteiro, L. C., & Costa, C. M. (2018). Clinical and laboratory assessment of Mangalarga Marchador horses submitted to marcha exercise. Brazilian Journal of Veterinary Medicine, 40, e47418. doi: 10.29374/2527-2179.bjvm47418

Comparison of gene expression of Toll-like receptors and cytokines between Piau and Commercial line (Landrace × Large White crossbred) pigs vaccinated against Pasteurella multocida type D

We aimed to compare Toll-like receptors (TLR) and cytokines expression in local Piau breed and a Commercial line (Landrace×Large White crossbred) pigs in response to vaccination against Pasteurella multocida type D. Seronegative gilts for Pasteurella multocida type D and Mycoplasma hyopneumoniae were used, from which peripheral blood mononuclear cells (PBMC) were collected in four time points (T0, T1, T2 and T3; before and after each vaccination dose). For bronchoalveolar lavage fluid cells (BALF), we set groups of vaccinated and unvaccinated animals for both genetic groups. Gene expression was evaluated on PBMC and BALF. In PBMC, when we analyzed time points within breeds, significant differences in expression for TLRs and cytokines, except TGFβ, were observed for Commercial animals. For the Piau pigs, only TGFβ showed differential expression. Comparing the expression among genetic groups, the Commercial pigs showed higher expression for TLRs after first vaccination dose, while for IL2, IL6, IL12 and IL13, higher expression was also observed in T3 and IL8 and IL10, in T1 and T3. Still comparing the breeds, the crossbred animals showed higher expression for TNFα in T1 and T2, while for TGFβ only in T2. For gene expression in BALF, vaccinated Commercial pigs showed higher expression of TLR6, TLR10, IL6, IL8, IL10, TNFα and TGFβ genes than vaccinated Piau pigs. The Commercial line pigs showed higher sensitivity to vaccination, while in local Piau breed lower responsiveness, which may partly explain genetic variability in immune response and will let us better understand the tolerance/susceptibility for pasteurellosis.

Enteral Fluid Therapy in Horses: Effects of Maintenance Hypotonic Electrolyte Solutions Containing Maltodextrin, Sucrose, or Dextrose Administered in Continuous Flow

Abstract To evaluate the effects of maintenance hypotonic electrolyte solutions containing carbohydrates administered by nasoesophageal tube in continuous flow in horses during 12 hours on body weight, waist circumference, water intake, aspect and moisture content of the stool, frequency of defecation, urine density and urinary glucose, sodium, potassium, chloride, urea and creatinine, and urinary volume in horses. We used six mares randomly divided into three treatments in two Latin squares 6 × 3, concurrent, in mixed model. All solutions had the same composition (5 g of NaCl, 0.5 g of KCl, 200 mg of magnesium pidolate, and 1 g of calcium gluconate, diluted in 1,000 mL of water), only differing in power source and osmolarity, ESMaltodextrin—electrolyte solution plus 15 g of maltodextrin (measured osmolarity of 203 mmol L−1); ESSucrose—electrolyte solution plus 15 g of sucrose (measured osmolarity of 234 mmol L−1); and ESDextrose—electrolyte solution plus 15 g of dextrose (measured osmolarity of 264 mmol L−1). The electrolyte solutions were administered at a dose of 15 mL kg−1 h−1 for 12 hours in continuous flow. Maintenance hypotonic electrolyte solutions containing maltodextrin, dextrose, and sucrose were effective in promoting diuresis and decreasing urinary density, without presenting adverse effects. ESSucr was more effective in softening the stool. HighlightsWe compared the effects of maintenance hypotonic enteral electrolyte solutions administered in continuous flow in horses.Maintenance hypotonic electrolyte solutions containing maltodextrin, dextrose, and sucrose are effective in promoting diuresis and decreasing urinary density, without presenting adverse effects.Maintenance hypotonic enteral electrolyte solutions with maltodextrin, dextrose, and sucrose are effective in horses.

Perfil metabólico e ponderal de leitões submetidos a dietas com diferentes teores de fósforo em ambientes térmicos distintos

The objective of this work was to evaluate the effect of diets with different available phosphorus contents on metabolic profile and on body weight of piglets at baseline, maintained in distinct thermal environments. One hundred and twenty barrow piglets from commercial strain and with an average body weight of 15 kg were used. The treatments consisted of diets with 0.107, 0.214, 0.321, 0.428, and 0.535% available phosphorus, and of two environments: thermoneutral and warm. Body weight was determined at the beginning and at the end of the experimental period, and blood samples were taken for measuring electrolytes, albumin, total protein, nitrogenous substances, total and bone alkaline phosphatase, thyroid hormones, glucose, and lactate. Both diets and environments had effect on body weight gain and on the values of sodium, potassium, total calcium, magnesium, phosphorus, total and bone alkaline phosphatase, free T4, and lactate. The increase in phosphorus contents in the diet has a positive effect on body weight in the studied environments, but it unbalances blood calcium:phosphorus relations. Heat stress decreases the levels of glucose, total alkaline phosphatase, and free T4 in the blood, and increases those of lactate. Blood biochemical parameters reflect the metabolic state of the animals and can be used to evaluate nutritional balance and for early detection of metabolic alterations.