José Dantas Ribeiro Filho

Hemogasometria em eqüinos com compactação experimental do cólon maior tratados com sene, fluidoterapia enteral e parenteral

The blood acid-base status was studied in equines when induced large colon impaction was followed by applying an isotonic polionic enteral solution, or sene associated with intravenous fluid therapy, or fluid therapy alone. The blood samples were collected in twenty animals, four groups (E8, RL, SE, and C) of five animals each. Animals of the treated groups had experimentally induced colon impactions. Equines in group E8 received isotonic polionic enteral solution (8 mL kg -1 h -1 48

Hidratação enteral em equinos - solução eletrolítica associada ou não à glicose, à maltodextrina e ao sulfato de magnésio: resultados de laboratório

In the present study, the effects of four different electrolyte solutions on the packed cell volume (PCV), plasma volume and plasma concentrations of total protein, sodium, potassium, chloride, total magnesium, and ionized calcium in healthy and experimentally dehydrated horses were evaluated. Four crossbred horses, two males and two females were used. In experiment 1 (E1Des) the animals were experimentally dehydrated, while in the second experiment (E2Hig) healthy equines were used. In both experiments the animals were subjected to the following treatments: SE - isotonic electrolyte solution; SEGlu - glucose-enriched SE; SEMalt - maltodextrine-enriched SE and SEMg - magnesium sulphate-enriched SE. The electrolyte solutions used in all treatments were administered at 15mL kg-1 h-1 during 12h through small-bore nasoesophagus tube by continuous flow. The treatments with the electrolyte solutions resulted in an increase of plasma volume and a decrease in total plasma protein, hematocrit, while the electrolytes remained within the reference values.

Tratamentos da compactação experimental do cólon maior de equinos com hidratação enteral, intravenosa e sene (Cassia augustifolia Vahl)

Treatment of experimental large colon impaction in horses: enteral and parenteral fluid therapy and senna (Cassia augustifolia Vahl) The objective of this study was to investigate the effect of senna associated with intravenous fluid therapy (SEN), isotonic polionic enteral solution (PE) and intravenous fluid therapy (IV) alone in the treatment of experimental large colon impaction in horses. Fifteen horses with experimentally induced colon impaction were divided in three groups: Group PE received the isotonic polionic enteral solution (8 mL kg -1 h -1 , during 48 h); Group SEN received senna (20 mg kg -1 , two doses with 24 h interval) + Ringer lactate i.v . (10 mL kg -1 h -1 12 h -1 , for two days); and Group IV received Ringer lactate i.v. (16 mL kg -1 h -1 12 h -1 , for two days). The treatment protocol of group PE increased fecal hydration and helped remove impaction faster; however, prolonged intestinal hypomotility were observed. The treatment protocol SEN normalized the intestinal motility faster; however the time required to remove impaction was higher and caused higher increase in abdominal distention and pain, as well as less capacity to revert dehydration. The treatment used for group

Diagnosis of paratuberculosis in cattle: microbiological culture, serology and PCR

The aim of this study was to confirm clinical diagnosis of paratuberculosis in two cows showing suggestive clinical signs of the disease. Based on clinical signs, in culture and in IS900 PCR results from the individual milk samples it was possible to diagnose paratuberculosis in the cows studied.

Hemogasometria e ânion gap em equinos tratados com soluções eletrolíticas enterais contendo diferentes fontes de energia

The present study compared the effects of electrolyte solutions containing different sources of energy that were administrated through enteral route by naso-esophageal probe of small-caliber with continuous flow on the acid base balance in horses. Six adult females were used in two simultaneous 6x3 latin squares mixed model. The animals were divided into three groups and received the following treatments: SEDext - 5g of sodium chloride, 0.5g of potassium chloride, 0.2g of magnesium pidolate, 1g of calcium gluconate and 10g of dextrose diluted in 1.000mL of water. The osmolality measured was of 228mOsmol L-1; SEMalt - 5g of sodium chloride, 0.5g of potassium chloride, 0.2g of magnesium pidolate, 1g of calcium gluconate and 10g of maltodextrin diluted in 1.000mL of water. The osmolality measured was of 181mOsmol L-1 and SEProp - 5g of sodium chloride, 0.5g of potassium chloride, 0.2g of magnesium pidolate, 1g of calcium gluconate and 10g of calcium propionate diluted in 1.000mL of water. The osmolality measured was of 282mOsm L-1. The electrolyte solutions were administered in a dose of 15mL kg-1 h-1 for 12 hours. The treatments with enteral electrolyte solutions containing dextrose, maltodextrin and calcium propionate did not change blood gas analysis values in these animals.

Hemogasometria em cães com desidratação experimental tratados com soluções eletrolíticas comerciais administradas por via intravenosa

Three commercial intravenous electrolyte solutions were compared as for their effects on the blood acid-base status in dogs experimentally dehydrated by withholding water and inducing polyuria. Animals were randomly divided into three groups which were rehydrated with the following commercial electrolyte solutions during 12 hours: Lactate Ringer´s solution (RL), Ringer´s solution (RS) and a normal saline solution (0.9% sodium chloride) containing 5% dextrose (GF). The RL´s intravenous fluid therapy resulted in an alkalinizing effect demonstrated by a mild increase in arterial blood pH, ctCO2, bicarbonate (cHCO-3), and arterial blood base concentration (cBase) and, thus, can be used in animals exhibiting mild to moderate metabolic acidosis. In contrast, the RS and GF therapies led to a mild decrease in the concentration of arterial blood tritiable base (cBase) inducing an acidifying effect, which make them an option to treat dogs with metabolic alkalosis.

Soluções eletrolíticas enterais hipotônicas em equinos: efeitos de fontes de energia sobre determinados indicadores do equilíbrio ácido base

There were analyzed, in the following study, the effects of electrolytic hypotonic solutions that were administrated using small-caliber nasal esophageal probe with continual flow on acid-base balance, plasma lactate and urinary pH of horses . Six adult female were distributed in cross over system 6x3 on a mixed model simultaneously. The animals were distributed in three groups and each of them were managed by the following treatment: SEDext - 5g of sodium chloride, 5g of potassium chloride, 1g of calcium gluconate, 200mg of magnesium pidolate, and 15g of maltodextrin diluted on a 1.000mL of water. The m osmolarity measured was of 264mOsmol L-1. SEMalt - enteral electrolyte solution plus 15g of maltodextrin diluted on a 1.000mL of water. The osmolarity measured was of 203mOsmol L-1. SESucr - enteral electrolyte solution plus 15g of sucrose diluted on a 1,000mL of water. The osmolarity measured was of: 234mOsmol/L. The electrolytic solutions were administered at a dose of 15 ml kg-1 h-1 for 12 hours. Treatments associated with enteral electrolyte solutions associated to dextrose and maltodextrin did not change the average values of blood gas analysis, anion gap, measured strong ion difference and plasma lactate, while the administration of the three solutions caused a decrease in urinary pH values. Nevertheless, they are safe clinically to be used in horses.

Enteral fluid therapy through nasogastric tube in rumen cannulated goats

This paper reports the effects of fluid therapy in goats through nasogastric route with an electrolyte solution composed by concentrations of sodium, potassium and chloride similar to goat plasma (140mmol/L of Na+, 4.5mmol/L of K+, 110mmol/L of Cl-). Four Alpine Chamoisee goats, two of them with evident leakage of the rumen cannulas, were used in a crossover experimental design of two periods and two groups. In one group the two goats were submitted to a treatment protocol to induce dehydration before the fluid therapy, whereas the other group was not. Fluid therapy consisted supplying 10mL/kg/h of the electrolyte solution during 8 hours. No signs of discomfort or stress were observed. The dehydration model employed caused a mild dehydration indicated by decrease in feces humidity, body weight and abdominal circumference, and increase in plasma total solids concentration. During fluid therapy globular volume and plasma total solids decreased, whereas % body weight and abdominal circumference increased. No signs of hyperhydration were observed and serum electrolytes (Na+, Cl-, K+) presented no significant alterations in both groups. Fluid therapy proposed in this study was efficient to treat dehydration, even for rumen cannulated animals with evident leakage, and can be administrated safely with no electrolyte imbalance.

Concentração de anticoagulante, tempo e temperatura de armazenagem sobre os parâmetros hematológicos no hemograma automatizado

The study aimed to identify the effects of time, temperature of storage and excess of anticoagulant on hematological parameters of dogs. Blood samples of ten, clinically healthy dogs, of different breeds were utilized. Aliquots were stored with 1.8mg, 3.6mg, 7.2mg and 14.4mg of ethylenediaminetetraacetic acid (EDTA) per mL of blood, divided into two groups: 2°C to 8°C and room temperature. Right after collection, they were evaluated in four times: 0h, 12h, 24h and 48h. White blood cells, red blood cells, hemoglobin, packed cell volume (PCV), mean corpuscular volume (MCV), red cell distribution width (RDW), platelet and thrombocrit (PCT) were evaluated in the automatic cell counter. In the automatic cell counter analysis, the MCV increased significantly with higher concentrations of EDTA (7.2mg mL-1 and 14.4mg mL-1) peaking at 2.36%, in the highest concentration. Temperature and time also influenced the MCV, which showed a decrease in time 12 under temperature of 2°C to 8°C and an increase in time 24 and 48 at room temperature. Storage temperature influenced leukocytes and erythrocytes counts, which showed lower values at room temperature. Hemoglobin, PCV, platelet and PCT did not change significantly. The changes observed did not compromise the results obtained by automatic cell counter, showing that blood samples stored for two days remained in good condition for processing, especially when stored under refrigeration.

Effects of Enteral Fluid Therapy in Continuous Flow Administered by Nasogastric Tube in Buffalo Calves

To investigate the employment of enteral fluid therapy in continuous flow administered by a nasogastric tube in buffalo calves; toassesstheeffectsof a hypotonic and an isotonic electrolyte solutionon: vital functions, blood count and serum andurinary biochemistry profile of buffalo calves. Seven buffalo calves, clinically healthy, were submitted to two treatments. The solutions were administered in continuous flow through a nasogastric tube at a dose of 15 mL/kg/hr for 12 hours. The serum biochemistry profile showed an increase in chloride concentration, decrease in serumurea and osmolarity. In urine, anincrease in sodium and chloride concentrations and a decrease in calcium, creatinine and urea were observed. Enteral fluid therapy in continuous flow proved to beeasy to use and effective in maintaining volemia and concentration of electrolytes in buffalo calves.