Warren A. Dick

Soil carbon pools and fluxes in long-term corn belt agroecosystems

The dynamics of soil organic carbon (SOC) play an important role in long-term ecosystem productivity and the global C cycle. We used extended laboratory incubation and acid hydrolysis to analytically determine SOC pool sizes and fluxes in US Corn Belt soils derived from both forest and prairie vegetation. Measurement of the natural abundance of 13 C made it possible to follow the influence of continuous corn on SOC accumulation. The active pools (Ca) comprised 3 to 8% of the SOC with an average field mean residence time (MRT) of 100 d. The slow pools (Cs) comprised 50% of SOC in the surface and up to 65% in subsoils. They had field MRTs from 12‐28 y for C4-C and 40‐80 y for C3-derived C depending on soil type and location. Notill management increased the MRT of the C3-C by 10‐15 y above conventional tillage. The resistant pool (Cr) decreased from an average of 50% at the surface to 30% at depth. The isotopic composition of SOC mineralized during the early stages of incubation reflected accumulations of labile C from the incorporation of corn residues. The CO2 released later reflected 13 C characteristic of the Cs pool. The 13 C of the CO2 did not equal that of the whole soil until after 1000 d of incubation. The SOC dynamics determined by acid hydrolysis, incubation and 13 C content were dependent on soil heritage (prairie vs. forest), texture, cultivation and parent material, depositional characteristics. Two independent methods of determining C3 pool sizes derived from C3 vegetation gave highly correlated values. # 2000 Elsevier Science Ltd. All rights reserved.

Compost and compost water extract-induced systemic acquired resistance in cucumber and Arabidopsis.

ABSTRACT A biocontrol agent-fortified compost mix, suppressive to several diseases caused by soilborne plant pathogens, induced systemic acquired resistance (SAR) in cucumber against anthracnose caused by Colletotrichum orbiculare and in Arabidopsis against bacterial speck caused by Pseudomonas syringae pv. maculicola KD4326. A peat mix conducive to soilborne diseases did not induce SAR. The population size of P. syringae pv. maculicola KD4326 was significantly lower in leaves of Arabidopsis plants grown in the compost mix compared to those grown in the peat mix. Autoclaving destroyed the SAR-inducing effect of the compost mix, and inoculation of the autoclaved mix with nonautoclaved compost mix or Pantoea agglomerans 278A restored the effect, suggesting the SAR-inducing activity of the compost mix was biological in nature. Topical sprays with water extract prepared from the compost mix reduced symptoms of bacterial speck and the population size of pathogenic KD4326 in Arabidopsis grown in the peat mix but not in the compost mix. The peat mix water extract applied as a spray did not control bacterial speck on plants grown in either mix. Topical sprays with salicylic acid (SA) reduced the severity of bacterial speck on plants in the peat mix but did not further reduce the severity of symptoms on plants in the compost mix. The activity of the compost water extract was heat-stable and passed through a 0.2-mum membrane filter. beta-1,3-Glucanase activity was low in cucumber plants grown in either mix, but when infected with C. orbiculare, this activity was induced to significantly higher levels in plants grown in the compost mix than in plants grown in the peat mix. Similar results were obtained for beta-D-glucuronidase (GUS) activity driven by a PR2 (beta-1,3-glucanase) gene promoter in transgenic Arabidopsis plants grown in the compost or peat mix. GUS activity was induced with topical sprays of the compost water extract or SA in plants not inoculated with the pathogen, suggesting that compost-induced disease suppression more than likely involved the potentiation of resistance responses rather than their activation and that compost-induced SAR differed from SAR induced by pathogens, SA, or compost water extract.

Conservation tillage and macropore factors that affect water movement and the fate of chemicals.

A thorough understanding of how conservation tillage influences water quality is predicated on knowledge of how tillage affects water movement. This paper summarizes the effects of conservation tillage on water movement and quality mainly based on long-term experiments on Luvisols at the North Appalachian Experimental Watershed near Coshocton, OH, USA. Conservation tillage can have a much larger effect on how water moves through the soil than it does on the total amount percolating to groundwater. Soil macroporosity and the proportion of rainfall moving through preferential flow paths often increase with the adoption of conservation tillage and can contribute to a reduction in surface runoff. In some medium- and fine-textured soils most of the water that moves to the subsoil during the growing season (May-October) is probably transmitted by macropores. If a heavy, intense storm occurs shortly after surface application of an agricultural chemical to soils with well-developed macroporosity, the water transmitted to the subsoil by the macropores may contain significant amounts of applied chemical, up to a few per cent, regardless of the affinity of the chemical for the soil. This amount can be reduced by an order of magnitude or more with the passage of time or if light rainstorms precede the first major leaching event. Because of movement into the soil matrix and sorption, solutes normally strongly adsorbed by the soil should only be subject to leaching in macropores in the first few storms after application. Even under extreme conditions, it is unlikely that the amount of additional adsorbed solute transported to groundwater will exceed a few per cent of the application when conservation tillage is used instead of conventional tillage. In the case of non-adsorbed solutes, such as nitrate, movement into the soil matrix will not preclude further leaching. Therefore, when recharge occurs during the dormant season thorough flushing of the soil, whether macropores are present or not, can move the remaining solutes to groundwater. Thus, the net effect of tillage treatment on leaching of non-adsorbed solutes should be minimal.

Impacts of agricultural management practices on C sequestration in forest-derived soils of the eastern Corn Belt

Soil organic matter has recently been implicated as an important sink for atmospheric carbon dioxide (CO 2 ). However, the relative impacts of various agricultural management practices on soil organic matter dynamics and, therefore, C sequestration at spatial scales larger than a single plot or times longer than the typical three year experiment have rarely been reported. Results of maintaining agricultural management practices in the forest-derived soils of the eastern Corn (Zea mays L.) Belt states of Kentucky, Michigan, Ohio and Pennsylvania (USA) were studied. We found annual organic C input and tillage intensity were the most important factors in affecting C sequestration. The impact of rotation on C sequestration was primarily related to the way it altered annual total C inputs. The removal of above-ground plant biomass and use of cover crops were of lesser importance. The most rapid changes in soil organic matter content occurred during the first five years after a management practice was imposed with slower changes occurring thereafter. Certain management practices, e.g. no-tillage (NT), increased the soils ability to sequester atmospheric CO 2 . The impact of this sequestration will be significant only when these practices are used extensively on a large percentage of cropland and when the C-building practices are maintained. Any soil C sequestered will be rapidly mineralized to CO 2 if the soil organic matter building practices are not maintained.

Soil management and nitrous oxide emissions from cultivated fields in southern Ohio

Abstract Nitrous oxide (N 2 O) is an important atmospheric trace gas due to its involvement in the postulated global warming phenomenon and in the depletion of the ozone layer. Widespread concern has been triggered by recent reports of increased atmospheric N 2 O concentration. Since agriculture has been implicated as one contributor to that increase, a monitoring program was undertaken during the 1993 and 1994 cropping season (May–October) to evaluate the effect of several soil management practices on N 2 O emission from soil. Our results show that rates of N 2 O emission were generally near baseline levels during most sampling occasions. Major, but short-lived, fluxes of N 2 O were observed after rainfall events and during the days immediately following fertilizer application. It was during these times that most of the seasonal N 2 O loss occurred. An excellent relationship was found between seasonal N 2 O loss ( y ) and the maximum daily flux of N 2 O ( x ) during a season ( y = −0.4 x 2 + 43.1 x + 338, r 2 = 0.89, P 2 O emission data were log normally distributed for both years. Average daily emissions of N 2 O were 6.9 ± 6.3 g (range, 0.3 – 74.7 g) N 2 ON ha −1 day −1 and 17.6 ± 10.5 g (range, 0.1–326 g) N 2 ON ha −1 day −1 during the 1993 and 1994 seasons, respectively. Seasonal N 2 ON losses were, in general, highest in the continuous corn (CC) ( Zea mays L.) plots and lowest in the soybean ( Glycine max L.) plots of the corn/soybean/wheat ( Triticum aestivum L.)-hairy vetch ( Vicia villosa Roth) rotation (CSW-V). Average N loss as N 2 O during a cropping season was between 0.6 kg (for the soybean crop of the CSWV rotation and ridge till treatment) and 3.7 kg N 2 ON ha −1 year −1 , (for the CC rotation and the chisel till treatment). Approximately 0.5–3% of the inorganic N fertilizer added was lost as N 2 O. Our data show that seasonal N 2 ON loss from chisel-till plots were generally significantly higher than from no-till or ridge till plots.

Tillage systems and soil properties in North America

Abstract This paper reviews current knowledge on the range and extent of various tillage systems used in North America with special reference to the effects on soil properties, the erosion hazard and water quality. The increasing adoption of conservation-tillage systems since their introduction in the early 1960s follows an enhanced awareness of the increasing risks of soil erosion and non-point source pollution and the high cost of fuel with conventional tillage. Most “conventional tillage” systems encompass complete inversion tillage along with several secondary and tertiary tillage methods. In contrast, conservation-tillage systems involve streamlining various farm operations, thereby reducing the frequency and intensity of the soil manipulative operations. Reduction in tillage intensity has been accompanied by the development of rotations and cropping systems, methods of surface and internal drainage, fertilizer technology and pest management alternatives. The status of antecedent soil physical properties is an important factor affecting the choice of tillage systems. Important soil physical properties governing the choice of tillage systems include soil wetness and anaerobiosis, soil temperature and soil structure including its susceptibility to compaction, crusting or erosion. Tillage systems affect soil physical, chemical and biological properties. Among drastic tillage-induced changes in soil properties are bulk density, infiltration rate, aggregation and aggregate size distribution, soil organic carbon and nutrient profile, microbial activity and species diversity, and the population of earthworms. Macropores and biochannels are usually more prevalent in conservation-tillage than conventional-tillage systems. Conservation tillage induces stratification of soil organic matter and related nutrients, enhances the activity of soil fauna and leads to acidification. The magnitude of these changes depends on the soil type, the cropping systems and the type of conservation tillage adopted. Soil organic carbon and nutrient stratification are generally more pronounced in coarse-textures than in clayey soils. Conservation tillage is also associated with greater biomass pool size. The latter affects the nutrient response curves of the soil. Nitrification and denitrification are other important processes affected by tillage systems. The widespread adaption of conservation-tillage systems, although beneficial in controlling off-site sedimentation, has raised concerns about the potential for increased leaching of nutrients and pesticides to groundwater. Important pollutants associated with conservation tillage are nitrate and pesticides. Some studies have shown little effect of tillage on losses of pesticides. Conservation tillage may suppress crop yields, especially on heavy textured soil with poor internal drainage and in those prone to soil compaction caused by vehicular traffic.

Alkali-activated complex binders from class C fly ash and Ca-containing admixtures

Processes that maximize utilization of industrial solid wastes are greatly needed. Sodium hydroxide and sodium silicate solution were used to create alkali-activated complex binders (AACBs) from class C fly ash (CFA) and other Ca-containing admixtures including Portland cement (PC), flue gas desulfurization gypsum (FGDG), and water treatment residual (WTR). Specimens made only from CFA (CFA100), or the same fly ash mixed with 40 wt% PC (CFA60-PC40), with 10 wt% FGDG (CFA90-FGDG10), or with 10 wt% WTR (CFA90-WTR10) had better mechanical performance compared to binders using other mix ratios. The maximum compressive strength of specimens reached 80.0 MPa. Geopolymeric gel, sodium polysilicate zeolite, and hydrated products coexist when AACB reactions occur. Ca from CFA, PC, and WTR precipitated as Ca(OH)(2), bonded in geopolymers to obtain charge balance, or reacted with dissolved silicate and aluminate species to form calcium silicate hydrate (C-S-H) gel. However, Ca from FGDG probably reacted with dissolved silicate and aluminate species to form ettringite. Utilization of CFA and Ca-containing admixtures in AACB is feasible. These binders may be widely utilized in various applications such as in building materials and for solidification/stabilization of other wastes, thus making the wastes more environmentally benign.

Fluorescence microscopy for visualization of soil microorganisms—a review

Direct microscopic observation of microorganisms is an important tool in many microbial studies. Such observations have been reported for Protozoa, fungi, inoculated bacteria, and rhizosphere microorganisms but few studies have focused on indigenous bacteria and their spatial relationship within various microhabitats. Principles and applications of epifluorescence microscopy and confocal laser scanning microscopy for visualization of soil microorganisms in situ are reviewed. Both cationic and anionic dyes (also commonly referred to as fluorochromes if they are fluorescent) have been used based on their ability to bind to specific cellular components of microbial cells. Common fluorochromes used for imaging of microbial cells include acridine orange, ethidium bromide, fluorescein isothiocyanate, 5-(4,6-dichlorotriazinyl) aminofluorescein, 4′,6-diamidino-2-phenylindole, europium chelate, magnesium salt of 8-anilino-1-naphthalene sulfonic acid, and calcofluor white M2R. Combining fluorescence staining techniques with soil thin section technology allows one to obtain images of microorganisms in situ. Soil texture and the procedures used for resin embedding are important factors affecting the quality of stained soil thin sections. Indeed, general limitations of applying fluorescence microscopy to soil ecological studies are the non-specific binding of dyes to the soil matrix and the autofluorescence of some soil components. The development of fluorescent in situ hybridization and confocal laser scanning microscopy techniques provides new potential for microbial distribution studies.

Flue gas desulfurization by-products additions to acid soil: alfalfa productivity and environmental quality

Flue gas desulfurization (FGD) by-products are created when coal is burned and SO2 is removed from the flue gases. These FGD by-products are often alkaline and contain many plant nutrients. Land application of FGD by-products is encouraged but little information is available related to plant responses and environmental impacts concerning such use. Agricultural lime (ag-lime) and several new types of FGD by-products which contain either vermiculite or perlite were applied at 0, 0.5, 1.0, and 2.0 times the soils lime requirement (LR) rate to an acidic soil (Wooster silt loam). The highest FGD by-products application rate was equivalent to 75.2 Mg ha(-1). Growth of alfalfa (Medicago sativa L.) was significantly increased compared to the untreated control in the second year after treatment with yields for the 1 x LR rate of FGD approximately 7-8 times greater compared to the untreated control and 30% greater than for the commercial ag-lime. Concentrations of Mo in alfalfa were significantly increased by FGD by-products application, compared to the untreated control, while compared to the ag-lime treatment, concentrations of B increased and Ba decreased. No soil contamination problems were observed, even at the 2xLR rate, indicating these materials can be safely applied to agricultural soils.

Degradation of polycyclic aromatic hydrocarbons by microbial consortia enriched from three soils using two different culture media

A consortium composed of many different bacterial species is required to efficiently degrade polycyclic aromatic hydrocarbons (PAH) in oil-contaminated soil. We obtained six PAH-degrading microbial consortia from three oil-contaminated soils using two different isolation culture media. Denaturing gradient gel electrophoresis (DGGE) and sequence analyses of amplified 16s rRNA genes confirmed the bacterial community was greatly affected by both the culture medium and the soil from which the consortia were enriched. Three bacterial consortia enriched using malt yeast extract (MYE) medium showed higher degradation rates of PAHs than consortia enriched using Luria broth (LB) medium. Consortia obtained from a soil and then added back to that same soil was more effective in degrading PAHs than adding, to the same soil, consortia isolated from other, unrelated soils. This suggests that inoculum used for bioremediation should be from the same, or very similar nearby soils, as the soil that is actually being bioremediated.