H. A. J. Hoitink

Suppression of Plant Diseases by Composts

Composts offer unique opportunities to examine fundamental interactions between plant pathogens, biocontrol agents, soil organic matter and plant roots. These organic amendments can be prepared reproducibly and have the potential to provide consistent biological control of plant diseases. Foliar as well as root pathogens may be affected by composts. Unfortenately, many factors control these beneficial effects. Heat exposure during composting kills or inactivates pathogens if the process is monitored properly. Biocontrol agents with the exception of Bacillus spp. also are killed by this heat treatment, however. Thus, biocontrol agents largely must recolonize composts after peak heating. The raw feedstock, the composting environment, as well as conditions during curing and utilization affect the potential for recolonization of composts by biocontrol agents and the induction of disease suppression. In practice, controlled inoculation of compost with biocontrol agents has proved necessary to induce consistent levels of suppression.

Compost and compost water extract-induced systemic acquired resistance in cucumber and Arabidopsis.

ABSTRACT A biocontrol agent-fortified compost mix, suppressive to several diseases caused by soilborne plant pathogens, induced systemic acquired resistance (SAR) in cucumber against anthracnose caused by Colletotrichum orbiculare and in Arabidopsis against bacterial speck caused by Pseudomonas syringae pv. maculicola KD4326. A peat mix conducive to soilborne diseases did not induce SAR. The population size of P. syringae pv. maculicola KD4326 was significantly lower in leaves of Arabidopsis plants grown in the compost mix compared to those grown in the peat mix. Autoclaving destroyed the SAR-inducing effect of the compost mix, and inoculation of the autoclaved mix with nonautoclaved compost mix or Pantoea agglomerans 278A restored the effect, suggesting the SAR-inducing activity of the compost mix was biological in nature. Topical sprays with water extract prepared from the compost mix reduced symptoms of bacterial speck and the population size of pathogenic KD4326 in Arabidopsis grown in the peat mix but not in the compost mix. The peat mix water extract applied as a spray did not control bacterial speck on plants grown in either mix. Topical sprays with salicylic acid (SA) reduced the severity of bacterial speck on plants in the peat mix but did not further reduce the severity of symptoms on plants in the compost mix. The activity of the compost water extract was heat-stable and passed through a 0.2-mum membrane filter. beta-1,3-Glucanase activity was low in cucumber plants grown in either mix, but when infected with C. orbiculare, this activity was induced to significantly higher levels in plants grown in the compost mix than in plants grown in the peat mix. Similar results were obtained for beta-D-glucuronidase (GUS) activity driven by a PR2 (beta-1,3-glucanase) gene promoter in transgenic Arabidopsis plants grown in the compost or peat mix. GUS activity was induced with topical sprays of the compost water extract or SA in plants not inoculated with the pathogen, suggesting that compost-induced disease suppression more than likely involved the potentiation of resistance responses rather than their activation and that compost-induced SAR differed from SAR induced by pathogens, SA, or compost water extract.

A Quantitative Respirometric Method for Monitoring Compost Stability

Compost stability was quantified using dissolved oxygen (D. O.) respirometry during composting of municipal solid waste (MSW) in a pilot-scale system. Changes in stability of samples taken at various times during the composting process were verified with chemical and physical tests. Rates of change of oxygen level in air over a compost sample incubated in a flask at 37°C were converted to a rate of O2 uptake/(g volatile solids · hour). Oxygen uptake, determined with D. O. respirometry and converted to rates of dry matter loss, was correlated with actual rates, calculated from energy balances observed in the pilot-scale system. The method can be used either as a simple quality control measure or in a more complex way to calculate rates so that efficiency within or among composting facilities can be compared.

Effect of compost amendments on disease severity and yield of tomato in conventional and organic production systems

Field trials were conducted over 2 years to assess the effects of compost amendments on disease development in organic and conventional processing tomato (Lycopersicon esculentum L.) production systems. The incidence of anthracnose fruit rot was reduced in organic tomato plots amended with a high rate of composted cannery wastes compared with the incidence in nonamended control plots in 1998 when disease incidence was high. Marketable yield was increased by 33% in compost-amended organic plots. Plots amended with a high compost rate had more ripe fruit than the nonamended control. The incidence of anthracnose and of total disease on fruit was less on the cultivar OH 8245 than on Peto 696. Total fruit yield of OH 8245 but not Peto 696 in organic plots was increased by amendment with composted cannery wastes. In conventional tomato production, composted yard wastes increased disease severity on foliage both years but reduced bacterial spot incidence on fruit in 1997, when disease pressure was high. The incidence of anthracnose was not affected by composted yard wastes. Marketable and total fruit yields of Peto 696 were not increased in compost-amended conventional plots. The plant activator Actigard reduced foliar disease severity and the incidence of bacterial spot and anthracnose on fruit, while increasing yield of marketable fruit.

Suppression of Bacterial Spot of Tomato with Foliar Sprays of Compost Extracts Under Greenhouse and Field Conditions

The efficacy of foliar sprays with compost water extracts (compost extracts) in reducing the severity of bacterial spot of tomato caused by Xanthomonas vesicatoria was investigated. Extracts prepared from composted cow manure, composted pine bark, an organic farm compost, or composted yard waste, applied as foliar sprays on tomato transplants, resulted in a moderate but statistically significant reduction in the severity of bacterial spot. The population of X. vesicatoria in infected leaves was reduced significantly by extracts prepared from composted cow manure. Efficacy of the water extracts was not affected by oxygen concentrations in the suspension during extraction, compost maturity, or sterilization by filtration or autoclaving. The degree of control provided by foliar sprays with the most effective compost extracts did not differ from that obtained with the plant activator acibenzolar-S-methyl. In the field in two growing seasons, foliar sprays with compost water extracts did not reduce the severity of foliar diseases, including bacterial spot. During the 1997 season, when the severity of bacterial spot in the field was high, foliar sprays with compost water extracts significantly reduced the incidence of bacterial spot on tomato fruit. Amending plot soil with several rates of composted yard waste did not lead to additional control of fruit disease over those only sprayed with extracts. Foliar sprays with a mixture of chlorothalonil and copper hydroxide or with acibenzolar-S-methyl reduced the severity of bacterial spot as well as incidence of spot on fruit.

Systemic Resistance Induced by Trichoderma hamatum 382 in Cucumber Against Phytophthora Crown Rot and Leaf Blight

Phytophthora root rot, crown rot, leaf and stem blight, and fruit rot of cucumber can cause serious losses, and are difficult to control. Although composts can be used successfully for control of Phytophthora root rots, little is known about their effects on Phytophthora diseases of aboveground plant parts. This research shows that the severity of Phytophthora root and crown rot of cucumber caused by Phytophthora capsici was suppressed significantly in cucumber transplants produced in a composted cow manure-amended mix compared with those in a dark sphagnum peat mix. In split root bioassays, Trichoderma hamatum 382 (T382) inoculated into the compost-amended potting mix significantly reduced the severity of Phytophthora root and crown rot on paired roots in the peat mix. This effect did not differ significantly from that provided by a drench with benzothiadiazole (BTH) or mefenoxam (Subdue MAXX). Based on area under disease progress curves, T382 also significantly reduced the severity of Phytophthora leaf blight in transplants produced in the compost mix compared with controls not inoculated with T382. Efficacy of T382 did not differ significantly from that provided by a drench with BTH. T382 re-mained spatially separated from the pathogen in plants in both the split root and leaf blight bioassays, suggesting that these effects were systemic in nature.

Systemic Resistance Induced by Trichoderma spp.: Interactions Between the Host, the Pathogen, the Biocontrol Agent, and Soil Organic Matter Quality

ABSTRACT Several factors affect the ability of Trichoderma spp. to provide systemic disease control. This paper focuses on the role of the substrate in which plants are grown, resistance of the host to disease, and the ability of introduced Trichoderma inoculum to spread under commercial conditions. Several reports reveal that foliar disease control provided by Trichoderma spp. is more effective on plants grown in compost-amended media compared with in lower-in-microbial-carrying-capacity sphagnum peat media. In Rhododendron spp., host resistance affects control of Phytophthora dieback provided by Trichoderma spp. For example, T. hamatum 382 (T382) significantly (P = 0.05) suppressed the disease on susceptible cv. Roseum Elegans while plant vigor was increased. The disease was not suppressed, however, on highly susceptible cvs. Aglo and PJM Elite even though the vigor of these plants was increased. Using a strain-specific polymerase chain reaction assay under commercial conditions, it was demonstrated that introduced inoculum of T382 did not spread frequently from inoculated to control compost-amended media. Other Trichoderma isolates typically are abundant in control media within days after potting unless inoculated with a specific Trichoderma isolate. Thus, the low population of isolates that can induce systemic resistance in composting and potting mix environments may explain why most compost-amended substrates do not naturally suppress foliar diseases.

Hydrolysis of fluorescein diacetate in sphagnum peat container media for predicting suppressiveness to damping-off caused by Pythium ultimum

Abstract Microbial activity, based on the rate of hydrolysis of fluorescein diacetate (PDA), accurately predicted supprcssivcness to Pythium damping-off of cucumber in media containing light-colored. relatively nondecomposed Canadian sphagnum peat. Suppressiveness was directly related to the quantity of FDA hydrolysed. Organic matter in container media adsorbed fluorescein. Adsorption was directly related to peat decomposition level. In media prepared with dark, decomposed sphagnum peats, that are H3-H4 or higher on the von Post scale, adsorption of fluorescein was strong enough to interfere with the assay. Since such decomposed peats were consistently conducive, the procedure did not yield false readings even here.

Effect of potting mix microbial carrying capacity on biological control of rhizoctonia damping-off of radish and rhizoctonia crown and root rot of poinsettia.

ABSTRACT Potting mixes prepared with dark, highly decomposed Sphagnum peat, with light, less decomposed Sphagnum peat, or with composted pine bark, all three of which were colonized by indigenous microorganisms, failed to consistently suppress Rhizoctonia damping-off of radish or Rhizoctonia crown and root rot of poinsettia. Inoculation of these mixes with Chryseobacterium gleum (C(299)R(2)) and Trichoderma hamatum 382 (T(382)) significantly reduced the severity of both diseases in the composted pine bark mix in which both biocontrol agents maintained high populations over 90 days. These microorganisms were less effective against damping-off in the light and dark peat mixes, respectively, in which populations of C(299)R(2) declined. In contrast, crown and root rot, a disease that is severe late in the crop, was suppressed in all three types of mixes. High populations of T(382) in all three mixes late during the cropping cycle may have contributed to control of this disease.

Prospects for Composts and Biocontrol Agents as Substitutes for Methyl Bromide in Biological Control of Plant Diseases

Methyl bromide, an effective soil fumigant for control of soilborne plant pathogens, is scheduled to be phased out by 2005 because of its negative impacts on the environment. Many chemical alternatives to methyl bromide have been proposed but so far, none have proved as effective. Composts have long been recognized to provide a degree of control of diseases caused by soilborne plant pathogens. For this reason, disease-suppressive effects of composts have been investigated intensively over the past two decades. Many compost quality factors must be controlled to obtain consistent effects with these organic amendments. The composition of the organic matter from which the compost is prepared, the composting process itself, the stability or maturity of the compost, the quantity of available plant nutrients provided by the compost, loading rates, time of application, and other factors all must be controlled. These and other factors are reviewed in this paper. Despite these difficulties, the use of compost for d...