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Featured researches published by Werner Henkel.
Biological Chemistry | 1975
Werner Henkel; Eckhart Buddecke
The glucosyltransferase (UDP-glucose galactosylhydroxylsine collagen glucosyltransferase, EC 2.4.1.?.) was purified 50-fold from calf arterial tissue by ammonium sulfate precipitation, gel filtration and electrofocusing. The purified enzyme has a molecular weight of 72 000 and a requirement for Mn2. It resolves into two activity peaks when submitted to electrofocusing (isoelectric point at pH 4.2 and 8.1) or disc electrophoresis and exhibits a double pH optimum (pH 8.3 and 9.9). The enzyme was found to transfer glucose from UDP-glucose to the denatured forms of citrate-soluble calf skin collagen (I), the alphal chain (II) and the beta12 component (III) derived from it, and of an acetic-acid-souble collagen preparation (IV) obtained from alkali-treated calf arterial tissue. The Km values for the substrates were 1.67 X 10(-4) (I), 6.3 X 10(-4) (II), 3.3 X 10(-4) (III) and 2.8 X 10(-4) mol/l (IV), indicating that the enzyme has the greatest affinity for the calf skin collagen. The glucose transferred to hydroxylysine-linked galactose residues may be released subsequently by the action of a specific alpha-glucosidase purified from bovine spleen. The results support the assumtion that the glucosylation step in the course of the (pro-)-collagen biosynthesis depends on special structural features of the substrate and may be controlled by a specific alpha-glucosidase.
FEBS Journal | 1982
Werner Henkel; Robert W. Glanville
FEBS Journal | 1987
Werner Henkel; Robert W. Glanville; Dieter Greifendorf
FEBS Journal | 1976
Werner Henkel; Jürgen Rauterberg; Toni Stirtz
Biochemistry | 1999
Werner Henkel; Thomas Vogl; Hartmut Echner; Wolfgang Voelter; Claus Urbanke; Detlef Schleuder; Jürgen Rauterberg
Biological Chemistry | 1979
Peter P. Fietzek; Hartmut Allmann; Jürgen Rauterberg; Werner Henkel; Elmar Wachter; Klaus Kühn
Journal of Proteome Research | 2007
Werner Henkel; Klaus Dreisewerd
Analytical Chemistry | 2004
Klaus Dreisewerd; Andreas Rohlfing; Beatrice Spottke; Claus Urbanke; Werner Henkel
Biological Chemistry | 1976
Jürgen Rauterberg; Hartmut Allmann; Werner Henkel; Peter P. Fietzek
FEBS Journal | 1979
Werner Henkel; Jürgen Rauterberg; Robert W. Glanville