Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Werner Henkel is active.

Publication


Featured researches published by Werner Henkel.


Biological Chemistry | 1975

Purification and properties of UDP-glucose galactosylhydroxylysine collagen glucosyltransferase (EC 2.4.1.?) from bovine arterial tissue.

Werner Henkel; Eckhart Buddecke

The glucosyltransferase (UDP-glucose galactosylhydroxylsine collagen glucosyltransferase, EC 2.4.1.?.) was purified 50-fold from calf arterial tissue by ammonium sulfate precipitation, gel filtration and electrofocusing. The purified enzyme has a molecular weight of 72 000 and a requirement for Mn2. It resolves into two activity peaks when submitted to electrofocusing (isoelectric point at pH 4.2 and 8.1) or disc electrophoresis and exhibits a double pH optimum (pH 8.3 and 9.9). The enzyme was found to transfer glucose from UDP-glucose to the denatured forms of citrate-soluble calf skin collagen (I), the alphal chain (II) and the beta12 component (III) derived from it, and of an acetic-acid-souble collagen preparation (IV) obtained from alkali-treated calf arterial tissue. The Km values for the substrates were 1.67 X 10(-4) (I), 6.3 X 10(-4) (II), 3.3 X 10(-4) (III) and 2.8 X 10(-4) mol/l (IV), indicating that the enzyme has the greatest affinity for the calf skin collagen. The glucose transferred to hydroxylysine-linked galactose residues may be released subsequently by the action of a specific alpha-glucosidase purified from bovine spleen. The results support the assumtion that the glucosylation step in the course of the (pro-)-collagen biosynthesis depends on special structural features of the substrate and may be controlled by a specific alpha-glucosidase.


FEBS Journal | 1982

Covalent Crosslinking between Molecules of Type I and Type III Collagen

Werner Henkel; Robert W. Glanville


FEBS Journal | 1987

Characterisation of a type-I collagen trimeric cross-linked peptide from calf aorta and its cross-linked structure. Detection of pyridinoline by time-of-flight secondary ion-mass spectroscopy and evidence for a new cross-link

Werner Henkel; Robert W. Glanville; Dieter Greifendorf


FEBS Journal | 1976

Isolation of a Crosslinked Cyanogen‐Bromide Peptide from Insoluble Rabbit Collagen Tissue Differences in Hydroxylation and Glycosylation of the Crosslink

Werner Henkel; Jürgen Rauterberg; Toni Stirtz


Biochemistry | 1999

Synthesis and folding of native collagen III model peptides.

Werner Henkel; Thomas Vogl; Hartmut Echner; Wolfgang Voelter; Claus Urbanke; Detlef Schleuder; Jürgen Rauterberg


Biological Chemistry | 1979

The covalent structure of calf skin type III collagen. I. The amino acid sequence of the amino terminal region of the alpha 1(III) chain (position 1--222).

Peter P. Fietzek; Hartmut Allmann; Jürgen Rauterberg; Werner Henkel; Elmar Wachter; Klaus Kühn


Journal of Proteome Research | 2007

Cyanogen Bromide Peptides of the Fibrillar Collagens I, III, and V and Their Mass Spectrometric Characterization: Detection of Linear Peptides, Peptide Glycosylation, and Cross-Linking Peptides Involved in Formation of Homo- and Heterotypic Fibrils

Werner Henkel; Klaus Dreisewerd


Analytical Chemistry | 2004

Characterization of whole fibril-forming collagen proteins of types I, III, and V from fetal calf skin by infrared matrix-assisted laser desorption ionization mass spectrometry.

Klaus Dreisewerd; Andreas Rohlfing; Beatrice Spottke; Claus Urbanke; Werner Henkel


Biological Chemistry | 1976

Isolation and Characterization of CNBr Derived Peptides of the α1 (III) Chain of Pepsin-Solubilized Calf Skin Collagen

Jürgen Rauterberg; Hartmut Allmann; Werner Henkel; Peter P. Fietzek


FEBS Journal | 1979

Isolation of Crosslinked Peptides from Insoluble Human Leiomyoma

Werner Henkel; Jürgen Rauterberg; Robert W. Glanville

Collaboration


Dive into the Werner Henkel's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Thomas Vogl

University of Münster

View shared research outputs
Researchain Logo
Decentralizing Knowledge