Wesam Al Khateeb

DDB2 , DDB1A and DET1 Exhibit Complex Interactions During Arabidopsis Development

Damaged DNA-binding proteins 1 and 2 (DDB1 and DDB2) are subunits of the damaged DNA-binding protein complex (DDB). DDB1 is also found in the same complex as DE-ETIOLATED 1 (DET1), a negative regulator of light-mediated responses in plants. Arabidopsis has two DDB1 homologs, DDB1A and DDB1B. ddb1a single mutants have no visible phenotype while ddb1b mutants are lethal. We have identified a partial loss-of-function allele of DDB2. To understand the genetic interaction among DDB2, DDB1A, and DET1 during Arabidopsis light signaling, we generated single, double, and triple mutants. det1 ddb2 partially enhances the short hypocotyl and suppresses the high anthocyanin content of dark-grown det1 and suppresses the low chlorophyll content, early flowering time (days), and small rosette diameter of light-grown det1. No significant differences were observed between det1 ddb1a and det1 ddb1a ddb2 in rosette diameter, dark hypocotyl length, and anthocyanin content, suggesting that these are DDB1A-dependent phenotypes. In contrast, det1 ddb1a ddb2 showed higher chlorophyll content and later flowering time than det1 ddb1a, indicating that these are DDB1A-independent phenotypes. We propose that the DDB1A-dependent phenotypes indicate a competition between DDB2- and DET1-containing complexes for available DDB1A, while, for DDB1A-independent phenotypes, DDB1B is able to fulfill this role.

Cadmium, copper and zinc toxicity effects on growth, proline content and genetic stability of Solanum nigrum L., a crop wild relative for tomato; comparative study.

Plants like other organisms are affected by environmental factors. Cadmium, copper and zinc are considered the most important types of pollutants in the environment. In this study, a comparison of growth and biochemical parameters between the crop wild relative (CWR) Solanum nigrum versus its cultivated relative Solanum lycopersicum to different levels of Cu, Zn and Cd stress were investigated. The presence of ZnSO4 and CuSO4 in Murashige and Skoog medium affected severely many growth parameters (shoot length, number of roots and leaves, and fresh weight) of both S. nigrum and S. lycopersicum at high levels. On the other hand, CdCl2 significantly reduced most of the studied growth parameters for both species. S. nigrum exhibited higher tolerance than S. lycopersicum for all types of stress. In addition, results show that as stress level increased in the growing medium, proline content of both S. nigrum and S. lycopersicum increased. A significant difference was observed between the two species in proline accumulation as a result of stress. In addition, a higher accumulation rate was observed in the crop wild relative (S. nigrum) than in cultivated S. lycopersicum. Changes in Inter-simple sequence repeat (ISSR) pattern of CuSO4 treated S. nigrum and S. lycopersicum plants were also observed. In conclusion, based on growth and biochemical analysis, S. nigrum showed higher level of metals tolerance than S. lycopersicum which indicates the possibility of using it as a crop wild relative for S. lycopersicum.

Seasonal variation in essential oil yield and composition from Thymus vulgaris L. during different growth stages in the south of Jordan

The effect of plant space and time of harvesting on yield and quality of Thymus vulgaris was evaluated in Jordan. Thyme was cultivated in rows of 50 cm apart with inter-row spacing of 15, 30 or 45 cm and was grown at various development stages. Plants were harvested during different growth stages including vegetation, beginning of blooming, full blooming and fruit maturation. Results indicated that oil yields of thyme were affected by growth stage and inter-row spacing. The maximum oil yields was obtained by harvesting at the early growth stage, which was found superior to oil yield corresponding to the later stages of collection. With 45 cm inter-row spacing, the maximum oil yield was recorded when the samples were collected at growth stage. Indicated results showed that the chemical composition during various growth stages was characterised by high percentage of carvacrol and its corresponding monoterpenic hydrocarbon precursors ρ-cymene and γ-terpinene, and ether 1,4-cineol.

Status of Water Quality in King Talal Reservoir Dam, Jordan

This work intends to monitor and assess the current water quality conditions at King Talal Dam (KTD), Jordan, based on vertical profiles and longitudinal surveys. While the surface distribution of water quality parameters in KTD varied spatially, their variations showed no distinct trends. Lateral and horizontal variations in water quality in KTD are probably linked with photosynthesis, sampling period, closeness to Zarqa River and Wadi Rmemeen, and water depths, among other factors. The vast majority of water quality parameters in KTD dammed reservoir showed vertical profiles that either decrease slightly with increasing depth from the surface (temperature and dissolved oxygen) or remain relatively unchanged (major ions, trace elements). These results suggest that water has probably been vertically mixed. Data also demonstrated rock weathering as a dominant process governing the water chemistry of KTD reservoir water. Based on chlorophyll- a classification of Forsberg and Ryding [19], KTD reservoir is in a mesotrophic-eutrophic condition. Evaluation of water quality for irrigation use revealed that KTD reservoir water is suitable for irrigation purposes, except for Na% and total hardness.

Plants genotoxicity as pollution bioindicator in Jordan using comet assay

This study aimed to assess genotoxicity in wild plants grown in Jordan as a pollution bioindicator. Comet assay was used to evaluate the level of DNA damage in plants collected from different areas in Jordan. Significant differences in plant DNA damage index and frequency were observed among sites of collection. Results show that plants collected from Aqaba back road and Ghour Assaal had significantly higher damage values. In contrast, plants collected from Wadi Rum, Al Naqab Heights, Swaimeh/Deadsea and Alshoneh Aljanobyeh showed low levels of DNA damage. A similar trend was observed for lipid peroxidation rates. Furthermore, heavy metal analysis showed that plants collected from Aqaba back road and Aqaba airport had the highest Al, Cr, Fe, Cu, Zn, Cd and Pb contents. A significant correlation was observed between DNA Damage Index, DNA Damage Frequency, lipid peroxidation rate, soil Cu, Cd and Pb biomarkers, indicating that heavy metals pollution is a major source for genotoxicity in these plant species. Finally, our results approved the feasibility of using plants and Comet assay system as a diagnostic tool for pollution in any environment adversely affected by different pollution sources.This study aimed to assess genotoxicity in wild plants grown in Jordan as a pollution bioindicator. Comet assay was used to evaluate the level of DNA damage in plants collected from different areas in Jordan. Significant differences in plant DNA damage index and frequency were observed among sites of collection. Results show that plants collected from Aqaba back road and Ghour Assaal had significantly higher damage values. In contrast, plants collected from Wadi Rum, Al Naqab Heights, Swaimeh/Deadsea and Alshoneh Aljanobyeh showed low levels of DNA damage. A similar trend was observed for lipid peroxidation rates. Furthermore, heavy metal analysis showed that plants collected from Aqaba back road and Aqaba airport had the highest Al, Cr, Fe, Cu, Zn, Cd and Pb contents. A significant correlation was observed between DNA Damage Index, DNA Damage Frequency, lipid peroxidation rate, soil Cu, Cd and Pb biomarkers, indicating that heavy metals pollution is a major source for genotoxicity in these plant species. Finally, our results approved the feasibility of using plants and Comet assay system as a diagnostic tool for pollution in any environment adversely affected by different pollution sources.

Evaluation of in Vitro Salt Tolerance in Cucumis Prophetarum L., a Crop Wild Relative = تقييم تحمل نبات Cucumis Prophetarum L. المزروع في أنابيب الاختبار للملوحة

Salinity is the major abiotic factor that limits plant growth and productivity. In this study, we present an in vitro propagation protocol for Cucumis prophetarum L. a crop wild relative to cucumber and compare the effect of salinity on C. sativus and C. prophetarum. Results showed that Murashige and Skoog (MS) medium supplemented with 1 mg L-1 Kin and 0.5 mg L-1 NAA was the optimal medium for shoot proliferation and root induction, respectively. Interestingly, C. prophetarum plants showed better growth responses than C. sativus under salinity stress. Furthermore, increased salinity level decreased lipid peroxidation content and increased proline content in C. prophetarum, but C. sativus showed opposite responses. In addition, C. sativus microshoots accumulate more Na than C. prophetarum. In conclusion, this study showed that C. prophetarum is more salt tolerant than C. sativus, this could be used in further work to improve salt tolerance traits in cucumber.

Role of Arabidopsis ABF1 / 3 / 4 during det1 germination in salt and osmotic stress conditions

Key messageArabidopsis det1 mutants exhibit salt and osmotic stress resistant germination. This phenotype requires HY5, ABF1, ABF3, and ABF4.AbstractWhile DE-ETIOLATED 1 (DET1) is well known as a negative regulator of light development, here we describe how det1 mutants also exhibit altered responses to salt and osmotic stress, specifically salt and mannitol resistant germination. LONG HYPOCOTYL 5 (HY5) positively regulates both light and abscisic acid (ABA) signalling. We found that hy5 suppressed the det1 salt and mannitol resistant germination phenotype, thus, det1 stress resistant germination requires HY5. We then queried publically available microarray datasets to identify genes downstream of HY5 that were differentially expressed in det1 mutants. Our analysis revealed that ABA regulated genes, including ABA RESPONSIVE ELEMENT BINDING FACTOR 3 (ABF3), are downregulated in det1 seedlings. We found that ABF3 is induced by salt in wildtype seeds, while homologues ABF4 and ABF1 are repressed, and all three genes are underexpressed in det1 seeds. We then investigated the role of ABF3, ABF4, and ABF1 in det1 phenotypes. Double mutant analysis showed that abf3, abf4, and abf1 all suppress the det1 salt/osmotic stress resistant germination phenotype. In addition, abf1 suppressed det1 rapid water loss and open stomata phenotypes. Thus interactions between ABF genes contribute to det1 salt/osmotic stress response phenotypes.

Association Between MTHFR 677C>T Polymorphism and Vitamin B12 Deficiency: A Case-Control Study

Summary Background: Vitamin B12 (cobalamin) deficiency is a prevalent worldwide health concern. Several factors are associated with vitamin B12 deficiency including lifestyle, genetic predisposition, and malfunctions in the absorption and transport of vitamin B12. In the current case-control study, we aimed at investigating the association between MTHFR polymorphisms and vitamin B12 deficiency in a Jordanian population. Methods: Two polymorphic sites of the MTHFR gene (c.677C>T, rs1801133 and c.1286A>C, rs1801131) were analyzed using RFLP and DNA sequencing in a group of vitamin B12 deficient individuals (45 males and 55 females). As a control, 100 matching individuals (age and sex) with vitamin B12 levels > 200 ng/mL were also recruited for this study. Results: The MTHFR c.677C>T variant was significantly associated with vitamin B12 deficiency in individuals from northern Jordan. The frequency of the homozygous MTHFR c.677C>T genotype was significantly higher in B12 deficient individuals in comparison with the control group (X2 = 8.397, p = 0.0150). The T allele frequency showed significant association with vitamin B12 deficiency in the study population (OR= 1.684, 95% CI: 1.116 to 2.542, p = 0.017). On the other hand, the MTHFR c.1286A>C variant did not show significant association with vitamin B12 deficiency in the selected population. Conclusions: Our results showed a significant association between homozygous MTHFR c.677C>T variant and T allele frequencies and vitamin B12 deficiency in the Jordanian population.

Reservoir water quality: a case from Jordan

Jordan relies heavily on reservoirs building and development to cope with water supply challenges, where monitoring and assessment of reservoir water quality are critically important for the sustainable use of these water supplies. Mujib Dam is an important water supply source in central western Jordan. Evaluation of water quality parameters and their spatial distributions (vertical and horizontal) showed near-neutral pH values with nearly similar values from surface to bottom. The vertical profile of DO and TDS in the dammed reservoir showed slight decreasing trends with increasing depth. Although Ca, Mg, Na, and K concentrations varied slightly with depths, their variations showed no trends. Similarly, the vertical and horizontal distribution patterns of Cl, SO4, HCO3, NO3, and PO4 in Mujib reservoir water showed insignificant variations in surface water layer and relatively unchanged values or decreasing trends through the water column. Higher values of TN have been observed, especially in the western part, suggesting that agricultural activities and livestock farming in the upstream catchment are impacting water quality. Results revealed that weathering and dissolution of rocks are the major source of water chemistry. The majority of trace metal levels (Cd, Cr, Cu, Fe, Mn, Pb, Zn, Co, Ni, Sr, and B) in water showed relatively similar surface and bottom values. The concentrations of COD and BOD5 in surface water were relatively low with higher concentrations observed in the northwestern corner, coincided with higher levels of chlorophyll a. The average ratio of TN to TP in surface water suggests that phosphorus is the limiting factor for the algal blooms, whereas the average chlorophyll a level in surface water indicates oligo-mesotrophic water.

Integrating morphological characters, molecular markers, and distribution patterns to assess the identity of Blepharis species from Jordan

BackgroundBlepharis constitutes an important part of the vegetation of the Jordanian arid and semi-arid regions, yet whether one or more species of this genus occurs in the Jordanian area is uncertain. We addressed this question by assessing morphological characters and testing Inter-Simple Sequence Repeat (ISSR) markers from three populations of Blepharis: two northern (lower slopes of Kufranjah valley and the Dead Sea region) and one southern (Wadi al Yutm).ResultsShoots from randomly chosen Blepharis plants were harvested from each of the three populations for morphological and molecular analyses. In the northern populations, spikes were lax and bract width was significantly shorter than length of the longest lateral spine compared to the southern population. A multivariate linear discriminant analysis distinguished the northern populations from the southern one by internode length, bract width, longest lateral spine length, and bract width to spine length ratio. The ISSR analysis revealed that 44 markers across eight primers were polymorphic with major allele frequency of 83.6% and an average of 5.5 polymorphic markers per primer. The genetic resemblance among individuals ranged from 0.27 to 0.96. The three Blepharis populations were accordingly clustered into two distinct groups, similar to the analysis of morphological differences and corresponding with the “northern” and “southern” population designations.ConclusionsOur results strongly indicate the occurrence of two discrete Blepharis species in Jordan and reject the hypothesis that the genus is represented by only one species. We propose that the Blepharis species in Jordan are B. attenutata Napper (represented by the northern populations) and B. ciliaris (L.) B. L. Burtt (represented by the southern population). These findings are important for informing and revising floristic work within the region and an updated key has been included in our findings.