William H. McGonagle

Multifocal multiphoton microscopy (MMM) at a frame rate beyond 600 Hz

We introduce a multiphoton microscope for high-speed three-dimensional (3D) fluorescence imaging. The system combines parallel illumination by a multifocal multiphoton microscope (MMM) with parallel detection via a segmented high-sensitivity charge-couple device (CCD) camera. The instrument consists of a Ti-sapphire laser illuminating a microlens array that projects 36 foci onto the focal plane. The foci are scanned using a resonance scanner and imaged with a custom-made CCD camera. The MMM increases the imaging speed by parallelizing the illumination; the CCD camera can operate at a frame rate of 1428 Hz while maintaining a low read noise of 11 electrons per pixel by dividing its chip into 16 independent segments for parallelized readout. We image fluorescent specimens at a frame rate of 640 Hz. The calcium wave of fluo3 labeled cardiac myocytes is measured by imaging the spontaneous contraction of the cells in a 0.625 second sequence movie, consisting of 400 single images.

An integrated electronic shutter for back-illuminated charge-coupled devices

A novel electronic shutter has been integrated into the structure of a back-illuminated frame-transfer charge-coupled device (CCD) to permit short optical exposure times and to reduce the smear that occurs during the transfer of an image from the CCD detection area. The shutter consists of an n/sup +/ shutter drain placed in the vertical channel stop regions and stepped p-type buried layers formed by a high-energy implantation (1.0-1.5 MeV) located between the CCD n-type buried channel the and p substrate. These structures create electric fields that direct the photoelectrons to either the CCD detection region or the n/sup +/ shutter drain. The ratio of photons detected with the shutter open to photons detected with the shutter closed has been measured to be greater than 75000 for wavelengths below 540 nm. The corresponding shutter rise and fall times are less than 55 ns. >

Progress on color night vision: visible/IR fusion, perception and search, and low-light CCD imaging

We report progress on our development of a color night vision capability, using biological models of opponent-color processing to fuse low-light visible and thermal IR imagery, and render it in realtime in natural colors. Preliminary results of human perceptual testing are described for a visual search task, the detection of embedded small low-contrast targets in natural night scenes. The advantages of color fusion over two alterative grayscale fusion products is demonstrated in the form of consistent, rapid detection across a variety of low- contrast (+/- 15% or less) visible and IR conditions. We also describe advances in our development of a low-light CCD camera, capable of imaging in the visible through near- infrared in starlight at 30 frames/sec with wide intrascene dynamic range, and the locally adaptive dynamic range compression of this imagery. Example CCD imagery is shown under controlled illumination conditions, from full moon down to overcast starlight. By combining the low-light CCD visible imager with a microbolometer array LWIR imager, a portable image processor, and a color LCD on a chip, we can realize a compact design for a color fusion night vision scope.

IOTA: Recent Technology and Science ⁄

The Sydney University Stellar Interferometer (SUSI) is a long-baseline optical interferometer operating at an observatory near Narrabri in Australia. SUSI features a 640 m long North-South array with 11 fixed siderostat stations. New science from the Blue (400-500 nm) and from the recently commissioned Red (500-950 nm) fringe detectors will be presented. Recent technological developments, mainly associated with the new Red detection system, encompassing wavefront correction, fringe encoding, wavelength switching and data analysis strategies, are described.Closure-phase science and technology are dominant features of the recent activity at IOTA. Our science projects include imaging several spectroscopic binary stars, imaging YSOs including Herbig AeBe stars, detecting asymmetries in a large sample of Mira stars, and measuring water shells around Miras. Many technology projects were pursued in order to make these science observations possible. These include installation of a third-generation integrated-optics 3-beam combiner (IONIC), completion of the real-time control system software, installation of fringe-packet tracking software, use of narrow sub-H band filters, validation of the phase-closure operation, development of CPLD control of the science camera (PICNIC) and star-tracker camera (LLiST), installation of a new star-tracker camera, expansion of the observing facility, and installation of new semi-automated optical alignment tools.

640/spl times/480 back-illuminated CCD imager with improved blooming control for night vision

We describe a back-illuminated 640/spl times/480 CCD imager which operates at 30-Hz frame rates with 5 e/sup -/ noise and which is capable of high resolution down to near starlight illumination levels. A new process for fabricating a compact blooming control is also described.

IOTA: Recent technology and science

Closure-phase science and technology are dominant features of the recent activity at IOTA. Our science projects include imaging several spectroscopic binary stars, imaging YSOs including Herbig AeBe stars, detecting asymmetries in a large sample of Mira stars, and measuring water shells around Miras. Many technology projects were pursued in order to make these science observations possible. These include installation of a third-generation integrated-optics 3-beam combiner (IONIC), completion of the real-time control system software, installation of fringe-packet tracking software, use of narrow sub-H band filters, validation of the phase-closure operation, development of CPLD control of the science camera (PICNIC) and star-tracker camera (LLiST), installation of a new star-tracker camera, expansion of the observing facility, and installation of new semi-automated optical alignment tools.

LLiST - a new star tracker camera for tip-tilt correction at IOTA ∗

The tip-tilt correction system at the Infrared Optical Telescope Array (IOTA) has been upgraded with a new star tracker camera. The camera features a backside-illuminated CCD chip offering doubled overall quantum efficiency and a four times higher system gain compared to the previous system. Tests carried out to characterize the new system showed a higher system gain with a lower read-out noise electron level. Shorter read-out cycle times now allow to compensate tip-tilt fluctuations so that their error imposed on visibility measurements becomes comparable to, and even smaller than, that of higher-order aberrations.

Intrapixel response test system for multispectral characterization

We report on the design of a system used to measure the multispectral intrapixel response of imaging sensor arrays. An Airy disk spot size of approximately 4 micrometers has been achieved for wavelength bands that extend from the visible blue to near IR. The automated system does rapid intrapixel row and/or column spatial mapping of individual pixels as well as rastered 2D spatial scans over multi-pixel girds. Commercially available equipment including a photometric eyepiece, a reflective objects, programmable pushers, and light-emitting diodes are utilized in the system. Scanned results using the system are presented for both front- and back-illuminating charge-coupled device imagers. The intrapixel response of a front-illuminated device shows good correlation with the physical cross section of the devices tested.

An epitaxially-grown charge modulation device

A charge modulation device (CMD) has been fabricated in a p-type epitaxial layer grown from the buried-channel silicon region of a charge-coupled device (CCD). Construction of the CMD directly above the CCD buried-channel and over the oxidized CCD transfer gates lowers the effective sense capacitance while providing isolation of the CMD source/drain regions. Responsivity values of 28 and 66 /spl mu/V/e for feedback and no feedback conditions, respectively, were measured dynamically on test devices. Input-referred noise values of approximately four electrons r.m.s. were calculated from noise spectral density measurements assuming a low-pass filter 3 dB cutoff frequency of 5 MHz and correlated double sampling.

A 10MHz CCD time integrating correlator

A 32-stage CCD binary-analog time-integrating correlator with a dynamic range greater than 40dB will be described. Time-bandwidth product is 250.