William Stern

Methods for the carboxyl-terminal fluorescent labeling of peptides using solid phase peptide synthesis

Abstract Two general methods for labeling synthetic peptides with a 5-dimethylamino-1-napthalenesulfonyl (dansyl) group at the C-terminal residue using solid phase peptide synthesis (SPPS) are described. Dansylated peptides are ideal substrates for fluorometric proteolytic enzyme assays.