Wolfgang Saeger
University of Cologne
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Featured researches published by Wolfgang Saeger.
Journal of the Endocrine Society | 2018
Cora Ballmann; Anne Thiel; Hannah E Korah; Anna-Carinna Reis; Wolfgang Saeger; Stefanie Stepanow; Karl Köhrer; Guido Reifenberger; Christiane B. Knobbe-Thomsen; Ulrich J. Knappe; Ute I. Scholl
Abstract Gain-of-function somatic mutations in the ubiquitin specific protease 8 (USP8) gene have recently been reported as a cause of pituitary adenomas in Cushing disease. Molecular diagnostic testing of tumor tissue may aid in the diagnosis of specimens obtained through therapeutic transsphenoidal surgery; however, for small tumors, availability of fresh tissue is limited, and contamination with normal tissue is frequent. We performed molecular testing of DNA isolated from single formalin-fixed and paraffin-embedded (FFPE) tissue sections of 42 pituitary adenomas from patients with Cushing disease (27 female patients and 15 male patients; mean age at surgery, 42.5 years; mean tumor size, 12.2 mm). By Sanger sequencing, we identified previously reported USP8 missense mutations in six tumors. Targeted next-generation sequencing (NGS) revealed known or previously undescribed missense mutations in three additional tumors (two with two different mutations each), with mutant allele frequencies as low as 3%. Of the nine tumors with USP8 mutations (mutation frequency, 21.4%), seven were from female patients (mutation frequency, 25.9%), and two were from male patients (mutation frequency, 13.3%). Mutant tumors were on average 11.4 mm in size, and patients with mutations were on average 43.9 years of age. The overall USP8 mutation frequency in our cohort was lower than in previously described cohorts, and we did not observe USP8 deletions that were frequent in other cohorts. We demonstrate that testing for USP8 variants can be performed from small amounts of FFPE tissue. NGS showed higher sensitivity for USP8 mutation detection than did Sanger sequencing. Assessment for USP8 mutations may complement histopathological diagnosis.
The Journal of Clinical Endocrinology and Metabolism | 1992
Martin Reincke; Joachim Nieke; Gabriel P. Krestin; Wolfgang Saeger; Bruno Allolio; W. Winkelmann
Archive | 2016
Martin Reincke; Bruno Allolio; Wolfgang Saeger; W. Winkelmann
Endocrine Abstracts | 2018
Silviu Sbiera; Nikita Popov; Isabel Weigand; Jörg Flitsch; Luis Gustavo Perez-Rivas; Lyudmyla Taranets; Elisabeth Graf; Camelia-Maria Monoranu; Wolfgang Saeger; Christian Hagel; Marily Theodoropoulou; Gunther Stalla; Sabine Herterich; Cristina Ronchi; Timo Deutschbein; Martin Reincke; Tim M. Strom; Martin Fassnacht
Endocrine Abstracts | 2017
Patricia Crock; Dieter K. Lüdecke; Ulrich J. Knappe; Joerg Flitsch; Wolfgang Saeger
19th European Congress of Endocrinology | 2017
Isabel Weigand; Lisanne Knobloch; Jens T. Vanselow; Jörg Flitsch; Carmelia M Monoranu; Wolfgang Saeger; Christian Hagel; Sabine Herterich; Cristina Ronchi; Andreas Schlosser; Martin Fassnacht; Timo Deutschbein; Silviu Sbiera
18th European Congress of Endocrinology | 2016
Klaus Christian Mende; Jakob Matschke; Rolf Buslei; Michael Buchfelder; Rudolf Fahlbusch; Wolfgang Saeger; Joerg Flitsch
16th European Congress of Endocrinology | 2014
Dieter K. Lüdecke; Patricia Crock; Wolfgang Saeger
15th European Congress of Endocrinology | 2013
Dieter K. Lüdecke; Joerg Flitsch; Patricia Crock; Wolfgang Saeger
Archive | 2010
Matthias Haase; Theresa Cox; Elke Kaminski; Dieter K. Lüdecke; Wolfgang Saeger; Reimar Fritzen; Matthias Schott; Werner A. Scherbaum; Holger S. Willenberg