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Dive into the research topics where Xianghui Gong is active.

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Featured researches published by Xianghui Gong.


Archives of Medical Research | 2010

Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem cells.

Yan Huang; Xiaoling Jia; Ke Bai; Xianghui Gong; Yubo Fan

BACKGROUND AND AIMS Bone marrow mesenchymal stem cells (BMSCs) are a potential source of material for the construction of tissue-engineered cardiac grafts because of their potential to transdifferentiate into cardiomyocytes after chemical treatment or co-culture with cardiomyocytes. Recent evidence has shown that mechanical loads could regulate the BMSC differentiation into osteoblasts and endothelial cells through various signaling pathways. We investigated whether fluid shear stress (FSS), which is a mechanical load generated by fluid flow, can regulate rat BMSC (rBMSC) differentiation into cardiomyocytes. METHODS rBMSCs were isolated from marrow of rat femur and tibia using density gradient centrifugation combined with adhesion method and identified with surface marker, proliferation character and differentiation potential in vitro. Cultured rBMSCs with or without 5-azacytidine (5-aza) treatment were exposed to laminar shear stress with a parallel plate-type device and analyzed by RT-PCR, immunocytochemistry, FACS and Western-blotting for the cardiomyogenic differentiation. RESULTS Appropriate FSS treatment alone induced cardiomyogenic differentiation of rBMSCs, as confirmed by the expression of cardiomyocyte-related markers at both mRNA and protein levels. Furthermore, when rBMSC cultures were exposed to both FSS and 5-aza, expression levels of cardiomyocyte-related markers significantly increased to a degree suggestive of a synergistic interaction. CONCLUSIONS The results demonstrate that FSS is an important factor affecting cardiomyogenic differentiation of rBMSCs. This provides a new avenue for mechanistic studies of stem cell differentiation and a new approach to obtain more committed differentiated cells.


PLOS ONE | 2012

Effect of Cyclic Strain on Cardiomyogenic Differentiation of Rat Bone Marrow Derived Mesenchymal Stem Cells

Yan Huang; Lisha Zheng; Xianghui Gong; Xiaoling Jia; Wei Song; Meili Liu; Yubo Fan

Mesenchymal stem cells (MSCs) are a potential source of material for the generation of tissue-engineered cardiac grafts because of their ability to transdifferentiate into cardiomyocytes after chemical treatments or co-culture with cardiomyocytes. Cardiomyocytes in the body are subjected to cyclic strain induced by the rhythmic heart beating. Whether cyclic strain could regulate rat bone marrow derived MSC (rBMSC) differentiation into cardiomyocyte-like lineage was investigated in this study. A stretching device was used to generate the cyclic strain for rBMSCs. Cardiomyogenic differentiation was evaluated using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR), immunocytochemistry and western-blotting. The results demonstrated that appropriate cyclic strain treatment alone could induce cardiomyogenic differentiation of rBMSCs, as confirmed by the expression of cardiomyocyte-related markers at both mRNA and protein levels. Furthermore, rBMSCs exposed to the strain stimulation expressed cardiomyocyte-related markers at a higher level than the shear stimulation. In addition, when rBMSCs were exposed to both strain and 5-azacytidine (5-aza), expression levels of cardiomyocyte-related markers significantly increased to a degree suggestive of a synergistic interaction. These results suggest that cyclic strain is an important mechanical stimulus affecting the cardiomyogenic differentiation of rBMSCs. This provides a new avenue for mechanistic studies of stem cell differentiation and a new approach to obtain more committed differentiated cells.


Macromolecular Bioscience | 2013

In Vitro Evaluation of Combined Sulfated Silk Fibroin Scaffolds for Vascular Cell Growth

Haifeng Liu; Xili Ding; Yanxue Bi; Xianghui Gong; Xiaoming Li; Gang Zhou; Yubo Fan

A combined sulfated silk fibroin scaffold is fabricated by modifying a knitted silk scaffold with sulfated silk fibroin sponges. In vitro hemocompatibility evaluation reveals that the combined sulfated silk fibroin scaffolds reduce platelet adhesion and activation, and prolong the activated partial thromboplastin time (APTT), thrombin time (TT), and prothrombin time (PT). The response of porcine endothelial cells (ECs) and smooth muscle cells (SMCs) on the scaffolds is studied to evaluate the cytocompatibility of the scaffolds. Vascular cells are seeded on the scaffolds and cultured for 2 weeks. The scaffolds demonstrate enhanced EC adhesion, proliferation, and maintenance of cellular functions. Moreover, the scaffolds inhibit SMC proliferation and induce expression of contractile SMC marker genes.


Pflügers Archiv: European Journal of Physiology | 2013

Involvement of large conductance Ca 2+ -activated K + channel in laminar shear stress-induced inhibition of vascular smooth muscle cell proliferation

Xiaoling Jia; Jingyun Yang; Wei Song; Ping Li; Xia Wang; Changdong Guan; Liu Yang; Yan Huang; Xianghui Gong; Meili Liu; Lisha Zheng; Yubo Fan

The large conductance Ca2+-activated K+ (BKCa) channel in vascular smooth muscle cell (VSMC) is an important potassium channel that can regulate vascular tone. Recent work has demonstrated that abnormalities in BKCa channel function are associated with changes in cell proliferation and the onset of vascular disease. However, until today there are rare reports to show whether this channel is involved in VSMC proliferation in response to fluid shear stress (SS). Here we investigated a possible role of BKCa channel in VSMC proliferation under laminar SS. Rat aortic VSMCs were plated in parallel-plate flow chambers and exposed to laminar SS with varied durations and magnitudes. VSMC proliferation was assessed by measuring proliferating cell nuclear antigen (PCNA) expression and DNA synthesis. BKCa protein and gene expression was determined by flow cytometery and RT-PCR. The involvement of BKCa in SS-induced inhibition of proliferation was examined by BKCa inhibition using a BKCa specific blocker, iberiotoxin (IBTX), and by BKCa transfection in BKCa non-expressing CHO cells. The changes in [Ca2+]i were determined using a calcium-sensitive dye, fluo 3-AM. Membrane potential changes were detected with a potential-sensitive dye, DiBAC4(3). We found that laminar SS inhibited VSMC proliferation and stimulated BKCa channel expression. Furthermore, laminar SS induced an increase in [Ca2+]i and membrane hyperpolarization. Besides in VSMC, the inhibitory effect of BKCa channel activity on cell proliferation in response to SS was also confirmed in BKCa-transfected CHO cells showing a decline in proliferation. Blocking BKCa channel reversed its inhibitory effect, providing additional support for the involvement of BKCa in SS-induced proliferation reduction. Our results suggest, for the first time, that BKCa channel mediates laminar SS-induced inhibition of VSMC proliferation. This finding is important for understanding the mechanism by which SS regulates VSMC proliferation, and should be helpful in developing strategies to prevent flow-initiated vascular disease formation.


Journal of Biomechanics | 2012

Fluid shear stress regulates metalloproteinase-1 and 2 in human periodontal ligament cells: Involvement of extracellular signal-regulated kinase (ERK) and P38 signaling pathways

Lisha Zheng; Yan Huang; Wei Song; Xianghui Gong; Meili Liu; Xiaolin Jia; Gang Zhou; Luoping Chen; Ang Li; Yubo Fan

Matrix metalloproteinase (MMP)-1, 2, with their endogenous inhibitors, tissue inhibitor of metalloproteinase (TIMP)-1, 2 are critical for extracellular matrix remodeling in human periodontal ligament (PDL) and their expression are sensitive to mechanical stresses. Shear stress as the main type of mechanical stress in tooth movement is involved in matrix turnover. However, how shear stress regulates MMPs and TIMPs system is still unclear. In this study, we investigated the effect of fluid shear stress on expression of MMP-1, 2 and TIMP-1, 2 in human PDL cells and the possible roles of mitogen-activated protein kinases in this process. Three levels of fluid shear stresses (6, 9 and 12 dyn/cm(2)) were loaded on PDL cells for 2, 4, 8 and 12h. The results indicated that fluid shear stress rearranged cytoskeleton in PDL cells. Fluid shear stress increased expression of MMP-1, 2, TIMP-1 and suppressed TIMP-2 expression. MAP kinases including extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 were activated rapidly by fluid shear stress. The ERK inhibitor blocked fluid shear stress induced MMP-1 expression and P38 inhibitor reduced fluid shear stress stimulated MMP-2 expression. Our study suggested that fluid shear stress involved in PDL remodeling via regulating MMP-1, 2 and TIMP-1, 2 expression. ERK regulated fluid shear stress induced MMP-1 expression and P38 play a role in fluid shear stress induced MMP-2 upregulation.


PLOS ONE | 2013

Galanin protects against nerve injury after shear stress in primary cultured rat cortical neurons.

Meili Liu; Wei Song; Ping Li; Yan Huang; Xianghui Gong; Gang Zhou; Xiaoling Jia; Lisha Zheng; Yubo Fan

The neuropeptide galanin and its receptors (GalR) are found to be up-regulated in brains suffering from nerve injury, but the specific role played by galanin remains unclear. This study aimed to explore the neuroprotective role of galanin after shear stress induced nerve injury in the primary cultured cortical neurons of rats. Our results demonstrated that no significant changes in cell death and viability were found after galanin treatment when subjected to a shear stress of 5 dyn/cm2 for 12 h, after increasing magnitude of shear stress to 10 dyn/cm2 for 12 h, cell death was significantly increased, while galanin can inhibit the nerve injury induced by shear stress with 10 dyn/cm2 for 12 h. Moreover, Gal2-11 (an agonist of GalR2/3) could also effectively inhibit shear stress-induced nerve injury of primary cultured cortical neurons in rats. Although GalR2 is involved in the galanin protection mechanism, there was no GalR3 expression in this system. Moreover, galanin increased the excitatory postsynaptic currents (EPSCs), which can effectively inhibit the physiological effects of shear stress. Galanin was also found to inhibit the activation of p53 and Bax, and further reversed the down regulation of Bcl-2 induced by shear stress. Our results strongly demonstrated that galanin plays a neuroprotective role in injured cortical neurons of rats.


Journal of Bioactive and Compatible Polymers | 2015

Preparation and characterization of silk fibroin/poly(l-lactide-co-ε-caprolactone) nanofibrous membranes for tissue engineering applications

Yuan Yao; Haifeng Liu; Xili Ding; Xiaohui Jing; Xianghui Gong; Gang Zhou; Yubo Fan

In recent years, silk fibroin has become one of the most promising tissue engineering materials because of its excellent cytocompatibility. Poly(l-lactide-co-ε-caprolactone), the copolymer of poly(l-lactide) and poly(ε-caprolactone), possesses good mechanical properties, and its degradation rates can be manipulated by varying the ratio of the constituent polymers. In this study, in order to combine their respective characteristics, silk fibroin/poly(l-lactide-co-ε-caprolactone) nanofibrous membranes were fabricated through electrospinning with different mass ratios of 100:0, 75:25, 50:50, 25:75, and 0:100. The surface properties, thermodynamic properties, mechanical properties, and cytocompatibility of silk fibroin/poly(l-lactide-co-ε-caprolactone) blended membranes were evaluated, and an optimal blending ratio was identified. The results showed that the silk fibroin/poly(l-lactide-co-ε-caprolactone) blended membranes containing 75% of silk fibroin and 25% of poly(l-lactide-co-ε-caprolactone) achieved the most improved performances compared with the single-component membranes or the blended membranes with other mixing ratios. The results from this study indicated that 75/25 silk fibroin/poly(l-lactide-co-ε-caprolactone) blended membranes which combined the advantages of poly(l-lactide-co-ε-caprolactone) and silk fibroin might be a suitable candidate material for use in tissue engineering.


Journal of Biomechanics | 2016

The effects of fluid shear stress on proliferation and osteogenesis of human periodontal ligament cells

Lisha Zheng; Luoping Chen; Yuchao Chen; Jinpeng Gui; Qing Li; Yan Huang; Meili Liu; Xiaolin Jia; Wei Song; Jing Ji; Xianghui Gong; Ruoshi Shi; Yubo Fan

Shear stress is one of the main stress type produced by speech, mastication or tooth movement. The mechano-response of human periodontal ligament (PDL) cells by shear stress and the mechanism are largely unknown. In our study, we investigated the effects of fluid shear stress on proliferation, migration and osteogenic potential of human PDL cells. 6dyn/cm(2) of fluid shear stress was produced in a parallel plate flow chamber. Our results demonstrated that fluid shear stress rearranged the orientation of human PDL cells. In addition, fluid shear stress inhibited human PDL cell proliferation and migration, but increased the osteogenic potential and expression of several growth factors and cytokines. Our study suggested that shear stress is involved in homeostasis regulation in human PDL cells. Inhibiting proliferation and migration potentially induce PDL cells to respond to mechanical stimuli in order to undergo osteogenic differentiation.


Journal of Bioactive and Compatible Polymers | 2016

Trilayered sulfated silk fibroin vascular grafts enhanced with braided silk tube

Xili Ding; Tongqiang Zou; Xianghui Gong; Changhui Ren; Hongyan Kang; Peng Xu; Haifeng Liu; Yubo Fan

The scaffold component is a major barrier to the development of a clinically useful small-diameter tissue-engineered vascular graft. Scaffold requirements include matching the mechanical and structural properties with those of native vessels and optimizing the microenvironment for cell integration, adhesion, and growth. Trilayered sulfated silk fibroin graft was developed to mimic native tissue structure and function. Physical properties and cell studies were assessed to evaluate the viability of their usage in small-diameter tissue-engineered vascular grafts. Compared with previously fabricated silk fibroin vascular grafts, these trilayered grafts provided comparable water permeability, tensile strength, burst pressure, as well as suture retention strength, to saphenous veins for vascular grafts. In addition, the in vitro results showed good cytocompatibility of the trilayered grafts. These physical and cellular outcomes indicate potential utility of these trilayered sulfated silk fibroin grafts for small-diameter vascular grafts.


Experimental Biology and Medicine | 2012

Stretch magnitude- and frequency-dependent cyclooxygenase 2 and prostaglandin E2 up-regulation in human endometrial stromal cells: possible implications in endometriosis.

Xiaochuan Li; Xianghui Gong; Lan Zhu; Leng Jh; Qingbo Fan; Dawei Sun; Jinghe Lang; Yubo Fan

Endometriosis, with a prevalence rate ranging from 6% to 10%, is the major contributor to pelvic pain and subfertility, and considerably reduces the quality of life in affected women. However, the pathogenesis of this disease remains largely unknown. The present study aimed to uncover the role of hyperperistalsis in the pathogenesis of endometriosis, by exploring the response of human endometrial stromal cells (ESCs) to the cyclic stretch in vitro. ESCs isolated from 18 different endometrium biopsies undergoing hysterectomy for myoma were subjected to uniaxial cyclic stretches with different magnitude and frequency using the Uniaxial Tension System. Expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E2 synthase-1 (mPGES-1) in stretched and unstretched ESCs were assessed by realtime quantitative polymerase chain reaction and Western blot. Production of prostaglandin E2 (PGE2) in the culture medium was measured by enzyme-linked immunosorbent assay. The cyclic stretch mimicking hyperperistalsis in endometriosis (5% elongation at 4 cycles/min) stimulated quick up-regulations of COX-2 and mPGES-1 simultaneously on both transcriptional and translational levels, and delayed PGE2 overproduction was also noted in ESCs. As the stretch magnitude or frequency increased, so did overexpression of COX-2 and PGE2 (P < 0.05). By contrast, the cyclic stretch mimicking physiological peristalsis (3% elongation at 2 cycles/min) did not induce significant COX-2, mPGES-1 or PGE2production within 12 h. Both COX-2 and mPEGS-1 are PGE2 synthases, and the aberrant COX-2 and PGE2 production play important roles in the pathogenesis of endometriosis. Therefore, the present findings revealed that increased stretch stimuli from the hyperperistalsis of endometriosis were capable of causing the aberrant COX-2 and PGE2 expression in the endometrium by mechanotransduction, in a magnitude and frequency-dependent manner. It implied possible roles of hyperperistalsis in the pathogenesis of endometriosis, particularly in the view of COX-2 and PGE2.

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