Xiaohang Ma
Zhejiang University
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Publication
Featured researches published by Xiaohang Ma.
Protein Expression and Purification | 2010
Xia Li; Tongcheng Xu; Hongbo Lu; Xiaohang Ma; Lei Kai; Kangping Guo; Yuhua Zhao
Purification of a cis-epoxysuccinic acid hydrolase was achieved by ammonium sulfate precipitation, ionic exchange chromatography, hydrophobic interaction chromatography followed by size-exclusion chromatography. The enzyme was purified 177-fold with a yield of 14.4%. The apparent molecular mass of the enzyme was determined to be 33kDa under denaturing conditions. The optimum pH for enzyme activity was 7.0, and the enzyme exhibited maximum activity at about 45 degrees C in 50mM sodium phosphate buffer (pH 7.5). EDTA and o-phenanthrolin inhibited the enzyme activity remarkably, suggesting that the enzyme needs some metal cation to maintain its activity. Results of inductively coupled plasma mass spectrometry analysis indicated that the cis-epoxysuccinic acid hydrolase needs Zn(2+) as a cofactor. Eight amino acids sequenced from the N-terminal region of the cis-epoxysuccinic acid hydrolase showed the same sequence as the N-terminal region of the beta subunit of the cis-epoxysuccinic acid hydrolase obtained from Alcaligenes sp.
Journal of Applied Microbiology | 2008
Weifeng Zhao; Xiaohang Ma; Xiaoming Jia; Y. Ma; Xia Li; Kangping Guo; Lei Kai; Xiangyang Xu
Aim: To investigate the possibility of finding a new homocysteine (Hcy) γ‐lyase with the desired properties for Hcy measurement in bacteria.
Bioresource Technology | 2008
Xia Li; Tongcheng Xu; Xiaohang Ma; Kangping Guo; Lei Kai; Yuhua Zhao; Xiaoming Jia; Yin Ma
World Journal of Microbiology & Biotechnology | 2005
Gui-Shan Zhang; Xiaoming Jia; Tian‐Fan Cheng; Xiaohang Ma; Yuhua Zhao
Process Biochemistry | 2005
Xue-lai Zhou; Xiaohang Ma; Guiqin Sun; Xia Li; Kangping Guo
Journal of Basic Microbiology | 2004
Tian‐Fan Cheng; Xiaoming Jia; Xiaohang Ma; Hai‐ping Lin; Yuhua Zhao
World Journal of Microbiology & Biotechnology | 2008
Lei Kai; Xiaohang Ma; Xue-lai Zhou; Xiaoming Jia; Xia Li; Kangping Guo
Fems Microbiology Letters | 2007
Xia Li; Xiaohang Ma; Yuhua Zhao; Xiaoming Jia; Lei Kai; Kangping Guo; Weifeng Zhao
Process Biochemistry | 2006
Yuanyuan Wang; Xiaohang Ma; Weifeng Zhao; Xiaoming Jia; Lei Kai; Xiaohong Xu
Asia Pacific Journal of Clinical Nutrition | 2008
Tongcheng Xu; Xia Li; Zhiguo Zhang; Xiaohang Ma; Duo Li