Xiaoxia Wen

The Relationship between Polyamines and Hormones in the Regulation of Wheat Grain Filling

The grain weight of wheat is strongly influenced by filling. Polyamines (PA) are involved in regulating plant growth. However, the effects of PA on wheat grain filling and its mechanism of action are unclear. The objective of the present study was to investigate the relationship between PAs and hormones in the regulation of wheat grain filling. Three PAs, spermidine (Spd), spermine (Spm), and putrescine (Put), were exogenously applied, and the grain filling characteristics and changes in endogenous PA and hormones, i.e., indole-3-acetic acid (IAA), zeatin (Z) + zeatin riboside (ZR), abscisic acid (ABA), ethylene (ETH) and gibberellin 1+4 (GAs), were quantified during wheat grain filling. Exogenous applications of Spd and Spm significantly increased the grain filling rate and weight, but exogenous Put had no significant effects on these measures. Exogenous Spd and Spm significantly increased the endogenous Spd, Spm, Z+ZR, ABA, and IAA contents and significantly decreased ETH evolution in grains. The endogenous Spd, Spm and Z+ZR contents were positively and significantly correlated with the grain filling rate and weight of wheat, and the endogenous ETH evolution was negatively and significantly correlated with the wheat grain filling rate and weight. Based upon these results, we concluded that PAs were involved in the balance of hormones that regulated the grain filling of wheat.

Effect of polyamines on the grain filling of wheat under drought stress.

Drought inhibits wheat grain filling. Polyamines (PAs) are closely associated with plant resistance due to drought and grain filling of cereals. However, little is known about the effect of PAs on the grain filling of wheat under drought stress. This study investigated whether and how PAs are involved in regulating wheat grain filling under drought stress. Two wheat genotypes differing in drought resistance were used, and endogenous PA levels were measured during grain filling under different water treatments. Additionally, external PAs were used, and the variation of hormone levels in grains was measured during grain filling under drought stress. The results indicated that spermidine (Spd) and spermine (Spm) relieve the inhibition caused by drought stress, and putrescine (Put) has the opposite effect. The higher activities of S-adenosylmethionine decarboxylase and Spd synthase in grains promotes the synthetic route from Put to Spd and Spm and notably increases the free Spd and Spm concentrations in grains, which promotes grain filling and drought resistance in wheat. The effect of PA on the grain filling of wheat under drought stress was closely related to the endogenous ethylene (ETH), zeatin (Z) + zeatin riboside (ZR) and abscisic acid (ABA). Spd and Spm significantly increased the Z + ZR and ABA concentrations and decreased the ETH evolution rate in grains, which promoted wheat grain filling under drought. Put significantly increased the ETH evolution rate, which led to excessive ABA accumulation in grains, subsequently aggravating the inhibition of drought on wheat grain filling. This means that the interaction of hormones, rather than the action of a single hormone, was involved in the regulation of wheat grain filling under drought.

Tillage, Mulch and N Fertilizer Affect Emissions of CO2 under the Rain Fed Condition

A two year (2010–2012) study was conducted to assess the effects of different agronomic management practices on the emissions of CO2 from a field of non-irrigated wheat planted on Chinas Loess Plateau. Management practices included four tillage methods i.e. T1: (chisel plow tillage), T2: (zero-tillage), T3: (rotary tillage) and T4: (mold board plow tillage), 2 mulch levels i.e., M0 (no corn residue mulch) and M1 (application of corn residue mulch) and 5 levels of N fertilizer (0, 80, 160, 240, 320 kg N/ha). A factorial experiment having a strip split-split arrangement, with tillage methods in the main plots, mulch levels in the sub plots and N-fertilizer levels in the sub-sub plots with three replicates, was used for this study. The CO2 data were recorded three times per week using a portable GXH-3010E1 gas analyzer. The highest CO2 emissions were recorded following rotary tillage, compared to the lowest emissions from the zero tillage planting method. The lowest emissions were recorded at the 160 kg N/ha, fertilizer level. Higher CO2 emissions were recorded during the cropping year 2010–11 relative to the year 2011–12. During cropping year 2010–11, applications of corn residue mulch significantly increased CO2 emissions in comparison to the non-mulched treatments, and during the year 2011–12, equal emissions were recorded for both types of mulch treatments. Higher CO2 emissions were recorded immediately after the tillage operations. Different environmental factors, i.e., rain, air temperatures, soil temperatures and soil moistures, had significant effects on the CO2 emissions. We conclude that conservation tillage practices, i.e., zero tillage, the use of corn residue mulch and optimum N fertilizer use, can reduce CO2 emissions, give better yields and provide environmentally friendly options.

The Effect of Plastic-Covered Ridge and Furrow Planting on the Grain Filling and Hormonal Changes of Winter Wheat

Although plastic-covered ridge and furrow planting(RF) has been reported to produce substantial increases in the grain weight of winter wheat,the underlying mechanism is not yet understood.The present study used two cultivars,Xinong 538 and Zhoumai 18,and RF and traditional flatten planting(TF,control) with the objective of investigating the effect of RF on wheat grain filling and the possible relationship of hormonal changes in the wheat grains under RF to grain filling.The results indicated that RF significantly increased the grain weight,although the effects on grain filling were different: RF significantly increased the grain-filling rate and grain weight of inferior grains,whereas RF had no significant effect on grainfilling rate and grain weight of superior grains.The final grain weight of inferior grains under RF was 39.1 and 50.7 mg for Xinong 538 and Zhoumai 18,respectively,3.6 and 3.4 mg higher than the values under TF.However,the final grain weight of superior grains under RF was only 0.6 and 0.8 mg higher than under TF for Xinong 538 and Zhoumai 18,respectively.RF significantly decreased the ethylene and gibberellic acid content in the inferior grains and increased the indole-3-acetic acid,abscisic acid and zeatin + zeatin riboside content in the inferior grains;however,no significant difference between RF and TF was observed for the hormonal content in the superior grains.Based on these results,we concluded that RF significantly modulated hormonal changes in the inferior grains and,thus,affected the grain filling and grain weight of the inferior grains;in contrast,RF had no significant effect on grain filling,grain weight and hormonal changes in the superior wheat grains.

Plastic-film mulching and urea types affect soil CO2 emissions and grain yield in spring maize on the Loess Plateau, China.

A 2-year field experiment was conducted on maize (Zea mays L.) to explore effective ways to decrease soil CO2 emissions and increase grain yield. Treatments established were: (1) no mulching with urea, (2) no mulching with controlled release fertiliser (CRF), (3) transparent plastic-film mulching (PMt) with urea, (4) PMt with CRF, (5) black plastic-film mulching (PMb) with urea, and (6) PMb with CRF. During the early growth stages, soil CO2 emissions were noted as PMt > PMb > no mulching, and this order was reversed in the late growth stages. This trend was the result of topsoil temperature dynamics. There were no significant correlations noted between soil CO2 emissions and soil temperature and moisture. Cumulative soil CO2 emissions were higher for the PMt than for the PMb, and grain yield was higher for the PMb treatments than for the PMt or no mulching treatments. The CRF produced higher grain yield and inhibited soil CO2 emissions. Soil CO2 emissions per unit grain yield were lower for the BC treatment than for the other treatments. In conclusion, the use of black plastic-film mulching and controlled release fertiliser not only increased maize yield, but also reduced soil CO2 emissions.

Effects of tillage management on soil CO2 emission and wheat yield under rain-fed conditions

Tillage disturbance can affect carbon dynamics in soil and plant production through several mechanisms. There are few integrated studies that have dealt with the effect of tillage management on soil CO2 emission and yield of wheat grain (Triticum aestivum L.) in the Loess Plateau in China. A 3-year (2010–12 and 2013–14) field experiment with two types of tillage was established to investigate CO2 emission, its related soil properties, crop yields and yield-scaled CO2 emissions (CO2 emissions per unit crop production) under rain-fed field conditions. Some land was planted with winter wheat without using tillage (‘no tillage’; NT), whereas some used mouldboard plough tillage (‘conventional tillage’; CT). The results indicate that CO2 was significantly and positively related to total nitrogen (P < 0.01), soil organic matter (P < 0.01), soil enzymes (P < 0.01; urease, invertase, and catalase), soil temperature (P < 0.01) and total pore space (P < 0.05). Multiple linear regression analysis in the NT plot included soil temperature and air filled pore space, explaining 85% (P < 0.05) of the CO2 variability, whereas in the CT plot the multiple linear regression model included soil temperature, urease, bulk density and pH, explaining 80% (P < 0.001) of the CO2 variability. Compared with the CT treatment, NT reduced the 3-year average yield-scaled CO2 emissions by 41% because of a 40% reduction in total CO2 emissions with no reduction in wheat yield. Thus, the results indicate that NT could be used to reduce the contribution of agriculture to CO2 emissions while simultaneously maintaining wheat crop production in this area.

Effect of plastic film mulching on the grain filling and hormonal changes of maize under different irrigation conditions.

Plastic film mulching (PM) is widely utilized for maize production in China. However, the effect of PM on the grain yield of crops has not been established, and the biochemical mechanism underlying the increase or decrease in grain yield under PM is not yet understood. Grain filling markedly affects the grain yield. The objective of this study was to investigate the effects of PM on maize grain filling under different irrigation levels and the relationship of such effects with hormonal changes. In the present study, PM was compared with traditional nonmulching management (TN) under 220 mm, 270 mm and 320 mm irrigation amount, and the grain filling characters of the grains located in various parts of the ear and the hormonal changes in the grains were measured. The results indicated that at 220 mm irrigation, PM significantly increased the grain filling rate of the middle and basal grains and decreased the grain filling rate of the upper grains. At 270 mm irrigation, the PM significantly increased the grain filling rate of the all grains. At 320 mm irrigation, the PM only significantly increased the grain filling rate of the upper grains. The IAA, Z+ZR and ABA content in the grains was positively correlated with the grain weight and grain-filling rates; however, the ETH evolution rate of the grains was negatively correlated with the grain weight and grain-filling rates. These results show that the effect of PM on maize grain filling is related to the irrigation amount and that the grain position on the ear and the grain filling of the upper grains was more sensitive to PM and irrigation than were the other grains. In addition, the PM and irrigation regulated the balance of hormones rather than the content of individual hormones to affect the maize grain filling.

Effect of polyamine on seed germination of wheat under drought stress is related to changes in hormones and carbohydrates

Drought is a multifaceted stress condition that inhibits crop growth. Seed germination is one of the critical and sensitive stages of plants, and its process is inhibited or even entirely prevented by drought. Polyamines (PAs) are closely associated with plant resistance to drought stress and seed germination. However, little is known about the effect of PA on the seed germination of wheat under drought stress. This study investigated the involvement of PAs in regulating wheat seed germination under drought stress. Six wheat genotypes differing in drought resistance were used, and endogenous PA levels were measured during seed germination under different water treatments. In addition, external PA was used for seed soaking and the variation of hormones, total soluble sugar and starch were measured during the seed germination under different water treatments. These results indicated that the free spermidine (Spd) accumulation in seeds during the seed germination period favored wheat seed germination under drought stress; however, the free putrescine (Put) accumulation in seeds during the seed germination period may work against wheat seed germination under drought stress. In addition, seed soaking in Spd and spermine (Spm) significantly relieved the inhibition of seed germination by drought stress; however, soaking seeds in Put had no significant effect on seed germination under drought. External Spd and Spm significantly increased the endogenous indole-3-acetic acid (IAA), zeatin (Z)+zeatin riboside (ZR), abscisic acid (ABA), and gibberellins (GA) contents in seeds and accelerated the seed starch degradation and increased the concentration of soluble sugars in seeds during seed germination. This may promote wheat seed germination under drought stress. In conclusion, free Spd and Put are key factors for regulating wheat seed germination under drought stress and the effects of Spd and Put on seed germination under drought notably related to hormones and starch metabolism.

Effect of Polyamine on Grain Filling of Winter Wheat and Its Physiological Mechanism

Grain filling in wheat is an important agronomic trait affecting grain weight.Polyamine is a plant growth regulator that is proved to participate in regulation of grain filling in rice and maize.In the present study,Zhoumai 18 and Xinong 538 were used to investigate the effect of polyamine on grain weight during filling process;besides,the photosynthetic characteristics,activities of antioxidant enzymes(SOD,POD,and CAT),malondialdehyde(MDA) content in flag leaves,and ethylene(ETH) content in grains were also measured for explaining the mechanism of such effect.The results showed that external spermidine(Spd) and spermine(Spm) significantly promoted grain filling,while external putrescine(Put) had no significant effect on grain filling.External Spm and Spd increased chlorophyll content,activities of antioxidant enzymes,and photosynthetic rate,but decreased MDA content in flag leaves and ETH level in grains.Therefore,we conclude that the effects on grain filling vary with polyamine species,and may relate to photosynthesis and senescence of leaves and ETH level of grains.

Conservation tillage enhances the stability of the rhizosphere bacterial community responding to plant growth

Soil microbes play important roles in plant growth and nutrient cycling. Conservation tillage is extensively used in northern China, which alters the soil structure and nutrient conditions, causing changes in the soil microbial community. However, the influence of conservation tillage on rhizosphere bacteria during plant growth, and associations with plant root nutrient efficiency, and plant productivity remains unclear, particularly regarding the effects on the stability of the soil bacterial community that modifies plant growth. Therefore, the aim of this study was to evaluate the contributions of conservation (chisel plow, zero) tillage and conventional (plow) tillage on the soil rhizosphere bacterial community throughout plant growth. The responses and succession of the bacterial community in the rhizosphere of winter wheat crops growing under conservation and conventional tillage practices were determined through high-throughput sequencing of 16S rRNA. The response of plant growth was determined by measuring plant carbon and nitrogen accumulation from the wheat tillering stage to the flowering stage. Here, we show that variations in rhizosphere alpha and beta diversity throughout plant growth had the greatest contributions to distinguishing conventional plow tillage from conservation (chisel plow, zero) tillage. Additionally, zero tillage (dissimilarity: 11.3%) had less of an effect on the relative abundances of Proteobacteria (Alpha-, Beta-, Gamma-) and Bacteroidetes in the rhizosphere in response to plant growth as compared with plow tillage (dissimilarity: 21.7%). This is the first in-depth study of the effects of conservation and conventional tillage on the stability of rhizosphere bacterial community in response to plant growth. Furthermore, our study indicates that conservation tillage can modify the soil conditions and preserve rhizosphere bacterial memberships. The consequent enhanced stability of the plant growth-promoting rhizobacteria population can help to establish a profitable agroecosystem to in turn enhance soil nutrient conditions and improve plant production sustainably.