Yahui Sun

Integrating planar waveguides doped with light scattering nanoparticles into a flat-plate photobioreactor to improve light distribution and microalgae growth.

Industrially manufactured planar waveguides doped with light scattering nanoparticles, which can dilute and redistribute the intense incident light within microalgae suspension more uniformly, were introduced into a flat-plate photobioreactor (PBR) with a width of 25cm to alleviate the adverse effect of poor light penetrability on microalgae growth. Compared with the flat-plate PBR without waveguides, the illumination surface area per unit volume in the proposed PBR was increased by 10.3 times. During the whole cultivation period, the illuminated volume fractions in the proposed PBR were 21.4-410% higher than those in the flat-plate PBR without waveguides. Consequently, attributed to the optimized light distribution in the proposed PBR, a 220% improvement in biomass production was obtained relative to that in the flat-plate PBR without waveguides. Furthermore, higher light output intensities emitted from the planar waveguide surfaces and increased microalgae growth rates were achieved by decreasing the length of planar waveguides.

Enhancement of microalgae production by embedding hollow light guides to a flat-plate photobioreactor

To offset the adverse effects of light attenuation on microalgae growth, hollow polymethyl methacrylate (PMMA) tubes were embedded into a flat-plate photobioreactor (PBR) as light guides. In this way, a fraction of incident light could be transmitted and emitted to the interior of the PBR, providing a secondary light source for cells in light-deficient regions. The average light intensity of interior regions 3-6cm from surfaces with 70μmolm(-2)s(-1) incident light was enhanced 2-6.5 times after 3.5days cultivation, resulting in a 23.42% increase in biomass production to that cultivated in PBR without PMMA tubes. The photosynthetic efficiency of microalgae in the proposed PBR was increased to 12.52%. Moreover, the installation of hollow PMMA tubes induced turbulent flow in the microalgae suspension, promoting microalgae suspension mixing. However, the enhanced biomass production was mainly attributed to the optimized light distribution in the PBR.

Effects of surface roughness on optical properties and sensitivity of fiber-optic evanescent wave sensors

The effects of surface roughness on the light transmission properties and sensitivity of fiber-optic evanescent wave sensors are investigated. A simple method of increasing the sensitivity based on the surface roughness (pit depth δ and diameter Δ) and incident angle U(i) of light rays on the fiber input end is proposed. We discovered that as 2δ/Δ increases, the transmitted light intensity decreases, but the sensitivity initially increases and then decreases. In sensors containing fibers of various roughnesses, the sensitivity to glucose solutions reached -11.7 mW/riu at 2δ/Δ=0.32 and increased further to -15.3 mW/riu with proper adjustment of U(i).

Comparison of Chlorella vulgaris biomass productivity cultivated in biofilm and suspension from the aspect of light transmission and microalgae affinity to carbon dioxide

To investigate light transmission and cells affinity to CO2, Chlorellavulgaris was attached to microfiltration membrane that laid on the solidified BG11 medium compared to that in suspended cultivation mode in this study. The results showed that C. vulgaris showed a 30.4% higher biomass production (103gm-2) in attached than in suspend system. The upper layer of biofilm with a thickness of 41.31μm (the corresponding areal density of 40gm-2) was effectively illuminated under light intensity of 120μmolm-2s-1 and more than 40% of the microalgal cells were in light even the areal density was high to 100gm-2. While only 2.5% of the cells were effectively illuminated in the suspended cultivation system. Furthermore, microalgae cells in biofilm showed a higher affinity to CO2 compared with that in suspension, and CO2 saturation point of microalgae cells in biofilm was 1.5% but 4.5% in suspension.

Improvement on light penetrability and microalgae biomass production by periodically pre-harvesting Chlorella vulgaris cells with culture medium recycling

To improve light penetrability and biomass production in batch cultivation, a cultivation mode that periodically pre-harvesting partial microalgae cells from suspension with culture medium recycling was proposed. By daily pre-harvesting 30% microalgae cells from the suspension, the average light intensity in the photobioreactor (PBR) was enhanced by 27.05-122.06%, resulting in a 46.48% increase in total biomass production than that cultivated in batch cultivation without pre-harvesting under an incident light intensity of 160μmolm(-2)s(-1). Compared with the semi-continuous cultivation with 30% microalgae suspension daily replaced with equivalent volume of fresh medium, nutrients and water input was reduced by 60% in the proposed cultivation mode but with slightly decrease (12.82%) in biomass production. No additional nutrient was replenished when culture medium recycling. Furthermore, higher pre-harvesting ratios (40%, 60%) and lower pre-harvesting frequencies (every 2, 2.5days) were not advantageous for the pre-harvesting cultivation mode.

Simultaneous enhancement of Chlorella vulgaris growth and lipid accumulation through the synergy effect between light and nitrate in a planar waveguide flat-plate photobioreactor

Interval between adjacent planar waveguides and light intensity emitted from waveguide surface were the primary two factors affecting light distribution characteristics in the planar waveguide flat-plate photobioreactor (PW-PBR). In this paper, the synergy effect between light and nitrate in the PW-PBR was realized to simultaneously enhance microalgae growth and lipid accumulation. Under an interval of 10mm between adjacent planar waveguides, 100% of microalgae cells in regions between adjacent waveguides could be illuminated. Chlorella vulgaris growth and lipid accumulation were synchronously elevated as light intensities emitted from planar waveguide surface increasing. With an identical initial nitrate concentration of 18mM, the maximum lipid content (41.66% in dry biomass) and lipid yield (2200.25mgL-1) were attained under 560μmolm-2s-1, which were 86.82% and 133.56% higher relative to those obtained under 160μmolm-2s-1, respectively. The PW-PBR provides a promising way for microalgae lipid production.

Boosting Nannochloropsis oculata growth and lipid accumulation in a lab-scale open raceway pond characterized by improved light distributions employing built-in planar waveguide modules

Aiming at alleviating the adverse effect of poor light penetrability on microalgae growth, planar waveguide modules functioned as diluting and redistributing the intense incident light within microalgae culture more homogeneously were introduced into a lab-scale open raceway pond (ORP) for Nannochloropsis oculata cultivation. As compared to the conventional ORP, the illumination surface area to volume ratio and effective illuminated volume percentage in the proposed ORP were respectively improved by 5.53 times and 19.68-172.72%. Consequently, the superior light distribution characteristics in the proposed ORP contributed to 193.33% and 443.71% increase in biomass concentration and lipid yield relative to those obtained in conventional ORP, respectively. Subsequently, the maximum biomass concentration (2.31 g L-1) and lipid yield (1258.65 mg L-1) was obtained when the interval between adjacent planar waveguide modules was 18 mm. The biodiesel produced in PWM-ORPs showed better properties than conventional ORP due to higher MUFA and C18:1 components proportions.

A novel self-adaptive microalgae photobioreactor using anion exchange membranes for continuous supply of nutrients

A novel self-adaptive microalgae photobioreactor using anion exchange membranes (AEM-PBR) for continuous supply of nutrients was proposed to improve microalgae biomass production. The introduction of anion exchange membranes to the PBR can realize continuous supply of nutrients at desired rates, which is beneficial to the growth of microalgae. The results showed that the maximum biomass concentration obtained in the AEM-PBR under continuous supply of nitrogen at an average rate of 19.0mgN/L/d was 2.98g/L, which was 129.2% higher than that (1.30g/L) in a PBR with all the nitrogen supplied in batch at initial. In addition, the feeding rates of nitrogen and phosphorus were optimized in the AEM-PBR to maximize biomass production. The maximum biomass concentration of 4.38g/L was obtained under synergistic regulation of nitrogen and phosphorus feeding rates at 19.0mgN/L/d and 4.2mgP/L/d. The AEM-PBR demonstrates a promising approach for high-density cultivation of microalgae.

Application of growth-phase based light-feeding strategies to simultaneously enhance Chlorella vulgaris growth and lipid accumulation

Considering the variations of optimal light intensity required by microalgae cells along with growth phases, growth-phase light-feeding strategies were proposed and verified in this paper, aiming at boosting microalgae lipid productivity from the perspective of light conditions optimization. Experimental results demonstrate that under an identical time-averaged light intensity, the light-feeding strategies characterized by stepwise incremental light intensities showed a positive effect on biomass and lipid accumulation. The lipid productivity (235.49 mg L-1 d-1) attained under light-feeding strategy V (time-averaged light intensity: 225 μmol m-2 s-1) was 52.38% higher over that obtained under a constant light intensity of 225 μmol m-2 s-1. Subsequently, based on light-feeding strategy V, microalgae lipid productivity was further elevated to 312.92 mg L-1 d-1 employing a two-stage based light-feeding strategy V560 (time-averaged light intensity: 360 μmol m-2 s-1), which was 79.63% higher relative to that achieved under a constant light intensity of 360 μmol m-2 s-1.

The kinetics of the polyacrylic superabsorbent polymers swelling in microalgae suspension to concentrate cells density

Different from current harvesting methods, the aim of this study was to concentrate microalgae by removing the medium with polyacrylic superabsorbent polymers (PSAPs). This method can concentrate freshwater microalgae Chlorella sp. at a relatively high biomass concentration of 90.23 g L-1. Without further dewatering, the concentrated microalgae can be directly used to produce biofuels by oil extraction or fermentation. The kinetic characteristics of PSAPs swelling in different solutions were investigated. The results indicate that the negative influence on absorbency caused by ionic strength was greater than microalgae concentration. Compared with the diffusion part, water absorbed by the relaxation of PSAPs was dominant and accounted for over 97%. Equilibrium absorbed water equations under different microalgae concentration were fitted and could provide guide to quantifiably concentrate microalgae. Increasing temperature decreased the absorbency of PSAPs, while, the absorption and desorption rate were increased. Moreover, the absorbency remained at 91.45% after recycling three times.