Yaozhou Zhang
Zhejiang Sci-Tech University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Yaozhou Zhang.
PLOS ONE | 2014
Jianqing Chen; Tejun Shu; Zhengbing Lv; Zuoming Nie; Jian Chen; Hao Chen; Wei Yu; Qijing Gai; Yaozhou Zhang
Human growth hormone (hGH) is a peptide hormone secreted by eosinophils of the human anterior pituitary, and a regulatory factor for a variety of metabolic pathways. A 30-kD protein from the pupa stage of silkworm was detected by Western blotting and confirmed by immunoprecipitation based on its ability to bind to anti-hGH antibody. This protein, named BmhGH-like protein, was purified from fresh silkworm pupas through low-temperature homogenization, filtration, and centrifugation to remove large impurity particles. The supernatants were precipitated, resuspended, and passed through a molecular sieve. Further purification by affinity chromatography and two-dimensional electrophoresis resulted in pure protein for analysis by MS MALDI-TOF-MS analysis. An alignment with predicted proteins indicated that BmhGH-like protein consisted of two lipoproteins, which we named hGH-L1 and hGH-L2. These proteins belong to the β-trefoil superfamily, with β domains similar to the spatial structure of hGH. Assays with K562 cells demonstrated that these proteins could promote cell division in vitro. To further validate the growth-promoting effects, hGH-L2 was cloned from pupa cDNA to create recombinant silkworm baculovirus vBmNPV-hGH-L2, which was used to infect silkworm BmN cells at low titer. Flow cytometric analysis demonstrated that the protein shortened the G0/G1 phase of the cells, and enabled the cells to rapidly traverse the G1/S phase transition point to enter S phase and promote cell division. Discovery of hGH-like protein in silkworm will once again arouse people’s interest in the potential medicinal value of silkworm and establish the basis for the development of new hormone drugs.
Archive | 2012
Lili Lu; Jianqing Chen; Tejun Shu; Yaozhou Zhang
Archive | 2012
Hao Chen; Man Ye; Jianqing Chen; Tejun Shu; Yaozhou Zhang
Archive | 2012
Shen Jun; Jianqing Chen; Tejun Shu; Yaozhou Zhang
Archive | 2012
Junqing Du; Jianqing Chen; Tejun Shu; Yaozhou Zhang
Archive | 2012
Xuesai Zhang; Jianqing Chen; Tejun Shu; Yaozhou Zhang
Archive | 2012
Xixi Shan; Jianqing Chen; Tejun Shu; Yaozhou Zhang
Archive | 2012
Yaozhou Zhang; Tejun Shu; Jianqing Chen; Shuwan Bai; Hao Chen
Protein Journal | 2015
Jianqing Chen; Tejun Shu; Jian Chen; Man Ye; Zhengbing Lv; Zuoming Nie; Qijing Gai; Wei Yu; Yaozhou Zhang
Archive | 2012
Man Ye; Hao Chen; Yaozhou Zhang; Jianqing Chen; Tejun Shu