Yaqin Wei
Xuzhou Medical College
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Yaqin Wei.
Pharmacology, Biochemistry and Behavior | 2012
Yao-Wu Liu; Xia Zhu; Wei Li; Qian Lu; Jian-yun Wang; Yaqin Wei; Xiaoxing Yin
OBJECTIVE This study was designed to investigate the effect of ginsenoside Re (Re) on cognitive functions, oxidative stress and inflammation in streptozotocin-induced diabetic rats. RESEARCH DESIGN AND METHOD Diabetic rats were treated with Re (40mg/kg) for 8weeks, blood glucose and body weight were measured monthly and weekly, respectively. Cognitive performances were evaluated with Morris water maze. Brain was obtained for measurements of TNF-α and malondialdehyde (MDA) contents in both temporal cortex and hippocampus, blood was collected for assays of TNF-α, MDA and reduced glutathione (GSH) levels. RESULTS Learning and memory abilities were significantly (both P<0.01) impaired in diabetic rats, accompanied by the marked (all P<0.01) elevations of TNF-α and MDA levels in temporal cortex and hippocampus. Increment of MDA and decrement of GSH in serum also occurred with significant differences (both P<0.01). Chronic treatment with Re markedly (P<0.05) improved the cognition of diabetic rats, evidenced by the decreased escape latency and the increased percentage of time spent in the target quadrant. Furthermore, Re treatment remarkably (P<0.05) reduced the levels of TNF-α and MDA in both brain areas of diabetic rats. Decline of MDA level and elevation of GSH level in serum were also seen in Re-treated diabetic rats, coupled with decrease in serum glucose level, all with statistically significant differences. CONCLUSIONS Our findings firstly provide the first evidence that ginsenoside Re can remarkably attenuate diabetes-associated cognitive decline, secondly confirm the involvement of oxidative stress and inflammation in the development of cognitive impairment caused by diabetes, finally point toward the potential of ginsenoside Re as an adjuvant therapy to conventional anti-hyperglycemic regimens as well as diabetes-associated cognitive decline.
Experimental Physiology | 2013
Qian Lu; Yun-peng Zhai; Qian Cheng; Yao-Wu Liu; Xing Gao; Ting Zhang; Yaqin Wei; Fan Zhang; Xiaoxing Yin
• What is the central question of this study? Oxidative stress is known to play an important role in the development and progression of diabetic nephropathy. However, the mechanism of overproduction of reactive oxygen species (ROS) in high‐glucose conditions is not completely clear. • What is the main finding and its importance? We demonstrated that high‐glucose concentrations induced excessive production of ROS and stimulated the phosphoinositide 3‐kinase–Akt–FoxO3a signalling pathway via the upregulation of transforming growth factor‐β1, resulting in phosphorylation and inactivation of FoxO3a and a reduction in the expression of its target gene, FoxO3a‐dependent manganese superoxide dismutase, and then further excessive production of ROS.
Journal of Ethnopharmacology | 2012
Yao-Wu Liu; Xia Zhu; Qian Lu; Jian-yun Wang; Wei Li; Yaqin Wei; Xiaoxing Yin
ETHNOPHARMACOLOGICAL RELEVANCE As a well-known Chinese Materia Medica Rhizoma Anemarrhenae has multiple pharmacological activities including antipyretic, anti-inflammatory, anti-diabetic actions, etc. This study was designed to investigate effects of total saponins from Rhizoma Anemarrhenae (TS) on diabetes-associated cognitive decline in rats and influence on amyloid-beta (Aβ) levels in brain and inflammation. MATERIALS AND METHODS Diabetic rats induced by intraperitoneal administration of streptozotocin, were randomized into two groups: diabetes and TS-treated diabetes. Blood glucose and body weight were measured monthly and weekly, respectively. After seven weeks, cognitive performances were evaluated with Morris water maze. Then, brain was obtained for assay of Aβ and TNF-α levels, and blood was collected for TNF-α assay. RESULTS Aβ(1-40), Aβ(1-42) and TNF-α levels were dramatically (all P<0.01) increased both in temporal cortex and hippocampus of diabetic rats, coupled with impairment of cognition, compared with those of the control. Chronic TS (200mg/kg) treatment markedly (P<0.05) improved the learning ability of diabetic rats, and significantly (all P<0.05) reduced Aβ(1-40), Aβ(1-42) and TNF-α levels in cortex as well as Aβ(1-40) level in hippocampus, whereas showed a decreased tendency for Aβ(1-42) and TNF-α levels in hippocampus. Moreover, eight-week treatment with TS remarkably (P<0.05) inhibited the elevation of TNF-α level in serum of diabetic rats, and significantly (both P<0.01) decrease the fasting blood glucose level and increase the body weight of diabectic rats. CONCLUSION Our findings demonstrate that diabetes-associated cognitive decline is, at least in part, due to brain Aβ accumulation in diabetic condition, and efficacy of TS to diabetes-associated cognitive decline in rats is a sum of reduction of Aβ accumulation and inflammation in brain as well as attenuation of major symptoms of diabetes.
Acta Pharmacologica Sinica | 2013
Yun-peng Zhai; Qian Lu; Yao-Wu Liu; Qian Cheng; Yaqin Wei; Fan Zhang; Chenglin Li; Xiaoxing Yin
Aim:To investigate whether NO over-production in rat mesangial cells cultured in high glucose (HG) is related to activation of the TGF-β1/PI3K/Akt pathway.Methods:Rat mesangial cells line (HBZY-1) was exposed to HG (24.44 mmol/L) or H2O2 (10 μmol/L) for 16 h. NO release was quantified using the Griess assay. The TGF-β1 level was measured using ELISA. The protein expression of p-Akt, t-Akt, Bim, and iNOS was examined by Western blotting. The mRNA levels of TGF-β1 and Bim were measured using RT-PCR. The cell proliferation rate was estimated using a BrdU incorporation assay.Results:Treatment of the cells with HG, H2O2, or TGF-β1 (5 ng/mL) significantly increased the NO level that was substantially inhibited by co-treatment with the NADPH oxidase inhibitor diphenylene iodonium (DPI), TGF-β1 inhibitor SB431542, or PI3K inhibitor LY294002. Both HG and H2O2 significantly increased the protein and mRNA levels of TGF-β1 in the cells, and HG-induced increases of TGF-β1 protein and mRNA were blocked by co-treatment with DPI. Furthermore, the treatment with HG or H2O2 significantly increased the expression of phosphorylated Akt and iNOS and cell proliferation rate, which was blocked by co-treatment with DPI, SB431542, or LY294002. Moreover, the treatment with HG or H2O2 significantly inhibited Bim protein and mRNA expression, which was reversed by co-treatment with DPI, SB431542, or LY294002.Conclusion:The results demonstrate that high glucose causes oxidative stress and NO over-production in rat mesangial cells in vitro via decreasing Bim and increasing iNOS, which are at least partially mediated by the TGF-β1/PI3K/Akt pathway.
Phytotherapy Research | 2011
Daoquan Tang; Yaqin Wei; Yuanyuan Gao; Xiaoxing Yin; Dongzhi Yang; Jie Mou; Xianglan Jiang
The protective effect of rutin on the glomerulosclerosis of diabetic nephropathy (DN) in rat mesangial cells was investigated. The cultured mesangial cells were divided into eight groups: normal, solvent control, high glucose, low dose of rutin, moderate dose of rutin, high dose of rutin, captopril and Ginkgo biloba extract. The cell cycles, type IV collagen and laminin in cytoplasm, TGF‐β1 mRNA of mesangial cells, Smad 2/3 and Smad 7, and the activities of four antioxidant indexes including T‐SOD, MDA, CAT and GSH‐Px were measured by flow cytometry, radioimmunoassay, RT‐PCR, western blotting and visible spectrophotometry, respectively. Compared with the high glucose group, rutin decreased the cell percentages of the G0/G1 phase and inhibited the expression of Smad 2/3, laminin and type IV collagen, and TGF‐β1 mRNA level, significantly. The antioxidant capacity, the cell percentages of S phase and Smad 7 expression were significantly increased by rutin. These results suggest that rutin is a potent protective agent against glomerulosclerosis in DN. Copyright
Journal of Ethnopharmacology | 2009
Daoquan Tang; Zunjian Zhang; Yuanyuan Gao; Yaqin Wei; Lin Han
ETHNOPHARMACOLOGICAL RELEVANCE Ginkgo biloba extract (GBE) is an ancient Chinese phytomedicine which was used to treat various ailments including circulatory and demential disorders. AIM OF THE STUDY To study GBEs protective effects on the glomerulosclerosis of diabetic nephropathy (DN) in rat mesangial cells by serum pharmacological methods. MATERIALS AND METHODS The cultured mesangial cells were divided into seven groups: normal, solvent control, high glucose, low dose of GBE serum, moderate dose of GBE serum, high dose of GBE serum, and GBE. The activities of cell antioxidases, type IV collagen and laminin, Smad2/3 and Smad7, and TGF-beta(1) mRNA were measured by methods of spectrophotometry, radioimmunoassay, immunocytochemistry, and RT-PCR, respectively. RESULTS The intensity of oxidative stress decreased in the GBE serum-treated groups in comparison with the high glucose group. In addition, the expression of Smad2/3 was greatly reduced, whereas Smad7 expression increased; type IV collagen, laminin and TGF-beta(1) mRNA levels were also diminished. CONCLUSION These results suggest that GBE is protective agent against glomerulosclerosis in diabetic nephropathy of mesangial cells.
Fitoterapia | 2011
Daoquan Tang; Yaqin Wei; Xiaoxing Yin; Qian Lu; Hui-hui Hao; Yun-peng Zhai; Jian-yun Wang; Jin Ren
Quercetins protective effects on the glomerulosclerosis of diabetic nephropathy (DN) in rat mesangial cells were investigated. The cell cycles, type IV collagen and laminin, TGF-β(1) mRNA, Smad 2/3 and Smad 7, and activities of cell antioxidases were measured. Compared with the high glucose group, quercetin may decrease the cell percentages of G(0)/G(1) phase, Smad 2/3 expression, laminin and type IV collagen, and TGF-β(1) mRNA level significantly. The antioxidant capacity, the cell percentages of S phase and Smad 7 expression was significantly increased by quercetin. These results suggest that quercetin is a protective agent against glomerulosclerosis in DN.
International Journal of Gynecological Cancer | 2011
Bei Zhang; Yao-Wu Liu; Peiying Zhang; Yaqin Wei; Xiaoxing Yin; Junnian Zheng
Objectives The aim of this study was to investigate the combined effects of a novel survivin promoter-based conditionally replicating adenovirus (CRAd-S.RGD) plus cis-diamminedichloroplatinum (cisplatin, CDDP) in ovarian cancer in vitro and in vivo. Methods The viability of human ovarian cancer cell line SKOV3 was determined by MTT assay following the infection with different doses of CRAd-S.RGD, either alone or in combination with CDDP. The antitumor efficacies and survival curves were evaluated at the end of the treatment regimens with the subcutaneous administration of CRAd-S.RGD, CDDP, combined therapy of CRAd-S.RGD plus CDDP, or phosphate-buffered saline in a SKOV3 xenograft animal model. Furthermore, the apoptosis rate of tumor tissues in mice was determined subsequent to the treatments. Results In vitro, the CRAd-S.RGD destroyed SKOV3 cells by oncolysis in a dose-dependent manner, and the viability of SKOV3 cells was significantly lower in the combined-therapy group than that in the individual-therapy groups. In vivo, enhanced tumor inhibition and animal survival rates were obtained in a synergistic manner with CRAd-S.RGD plus CDDP, as compared with the treatment with CRAd-S.RGD or CDDP alone. There was an increase in the apoptosis rate of the cells following the combined therapy. The results clearly demonstrated that there was a synergistic effect in the combination of CRAd-S.RGD and CDDP in increased therapeutic efficacy. Similar therapeutic efficacy could be obtained with CRAd-S.RGD plus CDDP at 2 lower doses that minimized the drug toxicity to host tissues. Conclusions The strategy of CRAd-S.RGD in combination with CDDP was a potential therapeutic modality for the therapy in ovarian cancer. Abbreviations CDDP - cisplatin, cis-diamminedichloroplatinum, CRAd - conditionally replicating adenovirus, CRAd-survivin - the survivin promoter-based conditionally replicating adenovirus, CRAd-S.RGD - CRAd–survivin-RGD4C, MOI - multiplicity of infection, PBS - phosphate-buffered saline, PI - propidium iodide
Family Medicine and Community Health | 2014
Xing Gao; Dongmei Lv; Yan Wang; Tao Wang; Wei Li; Yanbo Zhang; Yaqin Wei; Jiali Zhang; Qian Lu; Xiaoxing Yin
Objective This article aims to discuss the distribution of KCNQ1 gene polymorphism in the Chinese Han population in the Huaihai region of China and the correlation between KCNQ1 gene polymorphism and incidence of type 2 diabetes (T2DM). Methods From December 2010 to July 2011, 200 T2DM inpatients and outpatients in the Endocrinology Department of the Affiliated Hospital of Xuzhou Medical College were selected as the case group and, 200 healthy people identified by the health examination center in the same region were selected as the control group. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was used to examine the gene polymorphism of the two groups. Results (1) Analysis on the control group showed that at the KCNQ1 rC237892 locus, the genotype frequencies of CC, CT and TT were 36.0% (72/200), 51.0% (102/200) and 13.0% (26/200) respectively, and the allelic frequencies of C and T were 61.5% (246/400) and 38.5% (154/400) respectively. Analysis on the case group showed the genotype frequencies of CC, CT and TT were 47.5% (95/200), 44.0% (88/200) and 8.5% (17/200) respectively, and the allelic frequencies of C and T were 69.5% (278/400) and 30.5% (122/400) respectively. Comparison between the genotype distributions and allelic frequencies of the two tested groups at KCNQ1 rC237892 locus showed differences with statistical significance (P <0.05). (2) Comparison between the genotype distributions and allelic C and A frequencies of the control group and the case group showed differences with no statistical significance (P>0.05). Conclusion Polymorphism at KCNQ1 rs2237892 locus may be correlated to the incidence of T2DM in the Chinese Han population in Huaihai region of China; polymorphism at rsl51290 locus may be irrelevant to the incidence of T2DM in the Chinese Han population in Huaihai region of China.
Psychopharmacology | 2013
Yao-Wu Liu; Xia Zhu; Qian-Qian Yang; Qian Lu; Jian-yun Wang; Hui-Pu Li; Yaqin Wei; Jiale Yin; Xiaoxing Yin