Yegane Güven

Evaluation of oxidative stress and antioxidant profile in patients with oral lichen planus

OBJECTIVE The aim of this study was to assess oxidative stress and antioxidant profile in patients with oral lichen planus (OLP) using serum and salivary samples and to compare these biomarkers in a group of healthy subjects. PATIENTS AND METHODS Twenty-one recently diagnosed patients with OLP and 20 healthy controls with matched periodontal status were recruited to the study. Total antioxidant activity (TAA) and lipid peroxidation product malondialdehyde (MDA) in both serum and saliva were determined. Univariate comparisons between the two groups were made for quantitative and categorical variables to determine any significant differences. RESULTS In OLP patients, total antioxidant defense (TAA) was significantly lower than that in healthy subjects in their serum samples (P = 0.01). Salivary MDA levels were significantly higher in the OLP group compared with healthy subjects (P = 0.03). A significant correlation was found between serum and saliva TAA estimates in patients with OLP (r = 0.714 and P = 0.0001) and in the control group (r = 0.69 and P = 0.001). Significant correlation was also found between serum and saliva MDA values in control group (r = 0.464 and P = 0.04). A significant inverse correlation was found between salivary MDA and TAA values in the control group (r = -0.598 and P = 0.005). CONCLUSIONS The findings of this study suggest an increased oxidative stress and imbalance in the antioxidant defense system in biological fluids of patients with OLP. These findings may reflect the disease phenomenon of OLP rather than a causal effect and their role in pathogenesis and transformation of OLP to cancer, if any, needs further elucidation.

The effect of toxic doses of cholecalciferol (Vitamin D3) on the serum zinc levels in rats

Zinc is a trace element important to bone mineralization as well as, in general, nutrition. It is known that cholecalciferol (vitamin D3) affects bone metabolism. In this study toxic doses of vitamin D3 were injected subcutaneously (25 μg/d) to rats for 5 wk. It caused a significant increase in serum zinc levels (p<0.02). On the other hand, no significant increase was detected in the other groups. Excessive amounts of vitamin D3 caused bone breakdown and increased the levels of zinc in blood.

SCIENTIFIC BASIS OF DENTISTRY

Technological and scientific innovations have increased exponentially over the past years in the dentistry profession. In this article, these developments are evaluated both in terms of clinical practice and their place in the educational program. The effect of the biologic and digital revolutions on dental education and daily clinical practice are also reviewed. Biomimetics, personalized dental medicine regenerative dentistry, nanotechnology, high-end simulations providing virtual reality, genomic information, and stem cell studies will gain more importance in the coming years, moving dentistry to a different dimension.

DİŞLERİN BEYAZLATILMASINDA KULLANILAN KİMYASAL MADDELERİN AĞIZ DOKULARINA ETKİSİ-THE EFFECT OF TOOTH WHITENERS ON THE ORAL TISSUES

Ozet Son on yilda dis beyazlatici maddeler yaygin olarak kullanilmaktadir. Beyazlatma amaciyla, genellikle farkli konsantrasyonlarda hidrojen peroksit, sodyum perborat ve karbamid peroksit kullanilir. Bu derlemede, bu tip maddelerin agiz dokulari uzerine olan etkileri ozetlenmistir. Anahtar sozcukler: Dis beyazlaticilar, hidrojen peroksit, karbamid peroksit, agiz dokulari Abstract In the last decade, tooth-bleaching procedures have been used extensively. Materials such as hydrogen peroxide at different concentrations, sodium perborate and carbamide peroxide are generally used for bleaching purposes. In this article, the effects of these materials on the oral tissues is summarized. Key words: Tooth whiteners, hydrogen peroxide, carbamide peroxide, oral tissues.

10-15 YAŞ GRUBUNDAKİ ÇOCUKLARDA TÜKÜRÜK PEROKSİDAZ AKTİVİTESİ VE PLAK BİRİKİMİNİN İNCELENMESİ-THE EXAMINATION OF SALIVARY PEROXIDASE ACTIVITY AND PLAQUE ACCUMULATION OF CHILDREN AGED 10-15 YEARS

OZET Tukurugun antibakteriyel etki gosteren faktorlerinden biri de peroksidaz enzimidir. Bu calismada literaturde, gingivitis sikligi fazla oldugu belirtilen 10-15 yas grubundaki cocuklarda tukuruk peroksidaz aktivitesi ve plak birikimi tayin edilmistir. Deney grubunda ap-roksimal plak indeksi (API) ve diseti oldugu kanama indeksi (SBI) sirasiyla %55.78±24.2, %21.78 ± 16.5 olup, tukuruk peroksidaz aktivitesi 0.327 ± 0.09 IU/ml bulunmustur. Kontrol grubunda bu degerlerin %10.67 ± 3.98, %Q, 0.12 ± 0.032 IU/ml oldugu saptanmistir. Istatistiksel olarak, kontrol grubuna gore SBI sonuclari arasinda 0.001<p<0.01, API ve peroksidaz aktivitesinde p<0.001 duzeyinde anlamli farklar saptanmistir. Tukurukte peroksidaz aktivitesi tayininin periodontal hastaligin erken belirteci olarak kullanilabilecegi sonucuna varilmistir. Anahtar sozcukler: Tukuruk, peroksidaz, plak birikimi. Abbstract One of the antibacterial factor of saliva is the peroxidase enzyme. In this research, plaque accumulation and peroxidase activity were estimated of children aged 10-15 years who had a high prevalence of gingivitis which was emphasized in literature. It was found that approximal plaque index (API) was 55.78 ±24.2 %, sulcus bleeding index (SBI) was 21.78 ± 16.5% and salivary peroxidase activity was 0.327 i 0.09 IU/ml in tlie experimental group. Tliesevalu-es were detected respectively as 10.67 ± 3.98%, 0%, and 0.12 ± 0.032 IU in tlie control group. Statistically, significant differences were detected betweei SBI (0.001<p<0.01), API, salivary peroxidase activity (p<0.001) and control group. It was concluded that the estimation of salivary peroxidase activity might be a laboratory marker for periodontal disease. Key words: Saliva, peroxidase, plaque accumulation.

FLUORÜRLERİN SEMENT ÇÖZÜNÜRLÜĞÜ ÜZERİNE ETKİLERİ (*)-EFFECTS OF FLUORIDES ON SOLUBILITY OF CEMENTUM

Anahtar sozcukler : Kok yuzeyi curukleri,florurler, sement cozunurlugu Fluorurlerin mine cozunurlugunu azalttigi klinik ve laboratuvar calismalari ile gosterilmistir. Bu arastirmada fluorurlerin sement yuzeyi cozunurlugunu degistirip degistirmedigi saptanmaya calisildi. 20-35 yaslari arasindaki bireylerden cekilen 3. buyuk azi dislerinin kok yuzeyleri uzerinde arastirma yurutuldu. Dort ayri deney grubuna 3 veya 5 dakikalik NaF veya APF Jelleri uygulandi. Acikta kalan sement yuzeyleri 60 saniye HCI04 ile cozuldu ve cozeltide fosfor tayini yapildi. Elde edilen bulgulara gore 3 veya 5 dakikalik APF uygulamasinin kontrol grubuna oranla sement yuzeyi cozunurlugunu ileri derecede anlamli (P<0.001) olarak azalttigi goruldu. Key words : Root surface caries, fluorides, solubility of cementum In clinical and laboratory researches, it was shown that fluorides decrease the enamel solubility. In this study, whether or not the solubility of cementum can be changed by exporuse to fluorides. The laboratory work was performed on root surfaces of third molars extracted from individuals aged between 20-35 years. 3 or 5 minutes application of NaF or APF gels were used. Solubility of cementum was determined by detecting the phosphorus content of the etching solution of perchloric acid applied on the root surfaces. According to the findings obtained, it was observed that 3 or 5 minute application of APF decreased the solubility of cementum significantly (P<0.001) when compared with the control group.

The effect of heat-stable extract from bovine sublingual salivary glands on the carbohydrate metabolism of the rabbit

This extract (SLF3) caused hyperglycaemia when injected intraperitoneally (i.p.) into rabbits (20 mg/kg body wt) which was maximal at 0.5 h and took 2 h to return to the initial value. SLF3 also produced a two-fold increase in corticosteroid levels compared to controls, so the increase in blood glucose was probably mediated by adrenal cortical hormone stimulation.