Yin Wen

Combined treatment with bone marrow mesenchymal stem cells and methylprednisolone in paraquat-induced acute lung injury

BackgroundTo evaluate the efficacy of combined treatment with bone marrow mesenchymal stem cell (BMSC) transplantation and methylprednisolone (MP) to treat paraquat (PQ)-induced acute lung injury.Materials and methodsA total of 102 female rats were randomly divided into five groups: PQ, BMSC, MP, BMSC + MP and normal control. After 14 days of PQ poisoning, the survival of rats, wet/dry weight ratio of lung tissue, serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-10, malondialdehyde (MDA) and superoxidase dismutase (SOD), and the expression of nuclear factor (NF)-кB p65 in lung tissue were determined.ResultsRats in BMSC and BMSC + MP groups survived. BMSC transplantation significantly decreased the wet/dry weight ratio of lung tissue, down-regulated NF-кB p65 expression in lung tissue, lowered serum levels of TNF-α, IL-1β, IL-6 and MDA, and increased serum levels of IL-10 and SOD. These changes were particularly significant on days 7–14 after PQ poisoning. The above changes were more significant in the MP group on days 1–3 after PQ poisoning, compared with those of the BMSC group. However, the BMSC + MP group showed more significant changes on days 1–14 after PQ poisoning than those of both BMSC and MP groups.ConclusionsMP inhibits the inflammatory response, reduces the products of lipid peroxidation and promotes survival of transplanted BMSC, thus improving the intermediate and longer term efficacy of BMSC transplantation for treatment of PQ-induced lung injury.

Protection of bone marrow mesenchymal stem cells from acute lung injury induced by paraquat poisoning

Objective.u2003To examine the protective effect of bone marrow mesenchymal stem cell (BMSC) transplantation against lung injury induced by paraquat (PQ) poisoning. Materials and methods.u2003Fifty-four female SD rats were randomly divided into four groups: a PQ group, a BMSC treatment group, a BMSC control group, and a normal control group. BMSC, isolated and cultured in vitro, were injected into the tail veins of the rats. The mortality rate, the ratio of lung wet/dry weight, plasma levels of IL-1β, tumor necrosis factor-α (TNF-α), malonaldehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and NF-κBp65 expression in the lung tissues were examined during 14 days after injection. Results.u2003Survival in the PQ group ranged from 8 to 12 days, with an average of 9.6 days. All the rats in the BMSC treatment group survived for more than 14 days. In the BMSC treatment group, the ratio of lung wet /dry weight (Pu200a=u200a0.02u200a<u200a0.05), plasma IL-1β and TNF-α levels (Pu200a=u200a0.00u200a<u200a0.01), MDA levels (Pu200a=u200a0.03u200a<u200a0.05), and expression of NF-κBp65 in the lung tissues (Pu200a=u200a0.00u200a<u200a0.01) were significantly lower than those in the PQ group by day 7 post-injection. However, the SOD and GSH-PX levels began to increase (Pu200a=u200a0.00u200a<u200a0.01) compared with the PQ group. There was no significant difference between the BMSC control group and the normal control group (Pu200a>u200a0.01). Discussion and conclusion.u2003BMSC transplantation may have the potential to protect against PQ-induced lung injury, by reducing lung edema and lipid peroxidation, inhibiting the release of inflammatory mediators. However, the protective effects require time to develop, and early BMSC transplantation may be more evident during the middle and late stages of therapy.