Yishan Duan

Effects of Feral Haw (Crataegus pinnatifida B UNGE ) Seed Extracts on the Antioxidant Activities

Haw(Crataegus pinnatifida Bunge) belongs to the rose family and has beneficial health effects such as anti-obesity and gastroprotective activities. This study compared the antioxidative activities of various solvent extracts from haw. After removing seeds, pulpy was extracted with 70% methanol, 70% ethanol, chloroform :methanol(CM, 2:1, v/v), n-butanol, and ethyl acetate(EA). Total phenol contents were 71.46, 70.41, 47.63, 47.49 and 45.95 ㎎/g in the 70% methanol, 70% ethanol, CM, n-butanol and EA extracts, respectively. Total flavonoid contents of CM and n-butanol extracts were higher compared to other extracts at 1.63 ㎎/g and 1.59 ㎎/g, respectively, whereas EA extract had the lowest flavonoid content at 1.12 ㎎/g. Corresponding to total phenol contents, among NO2 radical scavenging activity, antioxidant activity by β-carotene bleaching assay, and superoxide dismutase(SOD)-like ability, 70% methanol and 70% ethanol extracts showed higher antioxidative activities than the other extracts. However, the lowest activities were n-butanol extract in NO2 radical scavenging activity as well as EA extract in antioxidant activity by β-carotene bleaching assay and SOD-like ability. These results suggest that haw extracts are available as a new natural antioxidant, and its activities are attributed to antioxidant substances such as phenolic compounds and flavonoids.

Screening of Antioxidant Activity from Exocarp of Watermelon (Citrullus vulgaris L.)

Abstract Watermelon ( Citrullus vulgaris L.) is a summer fruit typical to help fatigue systemic absorption is getting better. The goal of this study is to screen antioxidant activity to ensure the possibility as a functional material for exocarp of watermelon. Watermelon was extracted with 70% methanol, 70% ethanol, chloroform:methanol (CM, 2:1, v/v). Total phenol contents were 12.01 mg/g, 8.89 mg/g, 3.53 mg/g in the 70% methanol, 70% ethanol, CM, in that order, respectively. Total flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, ferric reducing antioxidant power (FRAP), β-carotene bleaching assay were 70% methanol extract remarkably higher than the other extracts. And these results showed the same trend of total phenol content. From the above results shows that watermelon was effective on the antioxidative activity. Key words : Citrullus vulgaris L., Exocarp, Antioxidative activity, Total phenol, Flavonoid 1. 서 론 1) 최근 90% 이상의 많은 질병이 단백질, 핵산과 같은 생체 분자를 손상시키는 reactive oxygen species (ROS)와 관련이 있다고 알려지면서, 이러한 활성산소를 제거하는 항산화 물질에 대한 연구가 활발하게 이루어지고 있다(Yang 등, 2008). 식물 속에 함유되어 있는 생리활성물질은 여러 화학반응에서 cofactor, inhibitor 및 scavenger 등으로 작용 하여 항산화, 항암, 항염증과 같은 활성을 나타낸다고 한다(Dillard와 German, 2000).수박(

Influences of Wild Haw (Crataegus pinnatifida B UNGE ) on Lowering BUN and Creatinine Concentrations in Dyslipidemia

Abstract This study was investigate the influences of wild haw ( Crataegus pinnatifida BUNGE) on lowering blood urea nitrogen (BUN) and creatinine concentrations in dyslipidemic rats. Four groups of rats were fed different diets for 5 weeks: basal diet (BD group), nondyslipidemic diet+feral haw extract (NDF group), dyslipidemic diet (DLD group), dyslipidemic diet+feral haw extract (DFH group). BUN, creatinine and uric acid concentrations were significantly higher in the DLD group than in the BD group. However, DFH group significantly lowered BUN, creatinine and uric acid concentrations compared to the DLD group. Amylase activity was significantly lower in the DLD group than in the BD group, while DFH group significantly higher compared to the DLD group. Lipase activity was also also significantly lower in the DLD group than the other group. Accordingly, these results suggest that haw extract could be effective for improving kidney function in dyslipidemic rats. Key words : Crataegus pinnatifida

Effects of Haw (Crataegus pinnatifida B UNGE ) on Relaxation in the Lipid Components and Blood Glucose of Lipid Metabolism Syndrome

Abstract This study was designed in order to determine the influences of haw ( Crataegus pinnatifida BUNGE) on the lipid metabolism syndrome. Sprague Dawley rats, 7 weeks old, were given four different types of diets for 5 weeks: ND group (noncholesterolemic diet), HE group (non cholesterolemic diet+haw extract), CD group (cholesterolemic diet), CH group (cholesterolemic diet+haw extract). Concentrations of LDL-cholesterol, triglyceride, phospholipid, free cholesterol, cholesteryl ester, blood glucose were remarkably higher in the CD group than the other groups. But by treatment of haw extract group with cholesterolemic diet (CH gr oup) were significantly decreased compared with CD group. Choleste ryl ester ratio was no difference between CD group and CH group. These results indicate that Crataegus pinnatifida BUNGE would be effective in lipid metabolism syndrome. Key words : Crataegus pinnatifida BUNGE, Lipid component, Blood glucose, Lipid metabolism syndrome 1. 서 론 1) 산사( Crataegus pinnatifida

Contents of Antioxidative Components from Pulpy and Seed in Wild Haw (Crataegus pinnatifida B UNGE )

Abstract This study was conducted to evaluate the contents of antioxidative components from pulpy and seed in wild haw ( Crataegus pinnatifida B UNGE ). Pulpy and seed of haw were smashed, then measured for color properties, antioxidative components of ascorbic acid, phytic acid, proanthocyanidin, anthocyanin, total carotene, β-carotene, lycopene, chlorophyll a, b and tannin. The a * , b * and C * values of seed were significantly lower than pulpy, but L * and H ° values were higher than that of pulpy. Ascorbic acid contents of pulpy and seed were found to be 10.89±1.69 mg/100 g and 1.45±0.16 mg/100 g, respectively. Phytic acid, proanthocyanidin, total carotene and tannin contents of pulpy and seed were 689.17±3.63 mg/g, 597.78±2.93 mg/g; 355.61±19.39 mg/g, 49.12±4.97 mg/g; 8.32±0.42 mg%, 0.80±0.01 mg%; 7.53± 0.09 mg/g, 1.02±0.03 mg/g, respectively. Similarly, β-carotene, lycopene, chlorophyll a and chlorophyll b contents of pulpy also displayed higher values than that of seed. On the contrary, anthocyanin content of seed (4.24±0.33 mg/L) was remarkably higher than pulpy (0.99±0.62 mg/L). The results showed that pulpy could be severed as great natural antioxidant and biohealth functional food.