Yoko Nakasone

New antioxidative phenolic glycosides isolated from Kokuto non-centrifuged cane sugar.

Nine compounds, 3-hydroxy-4,5-dimethoxyphenyl-β-D-glucopyranoside (1),β-D-fructfuranosyl-α-D-(6-vanilloyl)-glucopyranoside (2), β-D-fructfuranosyl-α-D-(6-syringyl)-glucopyranoside (3), 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxy-1-(E)-propenyl)- 2-methoxyphenoxy]propyl-β-D-glucopyranoside(4), 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3- hydroxy-1-(E)-propenyl)-2,6-dimethoxyphenoxy] propyl-β-D-glucopyranoside (5), dehydrodiconiferyl alcohol-9′-β-D-glucopyranoside (6), 4-[ethane-2-[3-(4-hydroxy-3-methoxyphenyl)-2-propen]oxy]-2,6-dimethoxyphenyl-β-D-glucopyranoside (7), 4-[ethane-2-[3-(4-hydroxy-3-methoxyphenyl)-2-propen]oxy]-2-methoxyphenyl-β-D-glucopyranoside (8), and 3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-2-[4-(3-hydroxy-1-(E)-propenyl)-2,6-dimethoxyphenoxy]propyl-β-D-glucopyranoside (9), were isolated from Kokuto non- centrifuged cane sugar. Their structures were elucidated by spectroscopic evidence, mainly based on the NMR technique. Among them, seven new glycosides were identified. The 2-deoxyribose oxidation method was used to measure their antioxidative activity. All of these compounds showed antioxidative activities.

New Phenolic Compounds from Kokuto, Non-centrifuged Cane Sugar

Five new phenolic compounds, 4-(β-D-glucopyranosyloxy)-3,5-dimethoxyphenyl-propanone (8), 3-[5-[(threo) 2,3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxybenzofurany]]-propanoic acid (12), 2-[4-(3-hydroxy-1-propenyl)-2,6-dimethoxyphenoxy]-3-hydroxy-3-(4-hydroxy-3,5-dimethoxyphenyl)propyl-β-D-glucopyranoside (13), 4-[(erythro) 2,3-dihydro-3(hydroxymethyl)-5-(3-hydropropyl)-7-methoxy-2-benzofuranyl]-2,6-dimethoxyphenyl-β-D-glucopyranoside (14), 9-O-β-D-xylopyranoside of icariol A2 (15), and known phenolic compounds were isolated from Kokuto, non-centrifuged cane sugar (Saccharum officinarum L.). Their structures were determined by a spectral investigation.

Efficacy of nucleoside-nucleotide mixture against methicillin-resistant Staphylococcus aureus infection in mice treated with cyclophosphamide

Abstract The effects of nucleoside-nucleotide mixture (NNM) and granulocyte-colony stimulating factor (G-CSF) on response to methicillin-resistant Staphylococcus aureus (MRSA) challenge in cyclophosphamide (CPA) treated mice were studied. Thirty BALB/c mice were randomly divided into 3 groups of 10 animals each and fed 20% nucleotide-free casein diet. On the 10th day on the diet, mice were intraperitoneally administered CPA and 4 hr later, inoculated intravenously with viable MRSA organisms. The mice were intraperitoneally administered saline (control group) or NNM daily from the onset of the experiment or injected G-CSF for 4 consecutive days beginning on the day after CPA injection. The cumulative percentage of survival over 10 days in the control group was 20% as compared to 60% and 80% in the NNM and G-CSF groups, respectively. Of the surviving mice, in the NNM group about half eliminated the MRSA organisms in spleen and kidney, as compared to none in the control and G-CSF groups. Our results suggest that administration of NNM as well as G-CSF may be used to an advantage to enhance the host resistance in CPA treated septic mice; and that NNM alone or in combination with other nutrients or pharmacologic agents may prove to be of clinical importance in the protection of immunocompromised patients against opportunistic pathogens.