Yolanda Cifuentes

Perfil microbiológico de aislamientos en unidades neonatales en un hospital de tercer nivel de Bogotá, Colombia

OBJECTIVES: Investigating the prevalence and sensitivity of germs isolated from newborn in a referral hospital in Bogota. Suggesting an empirical antibiotic treatment for neonatal infection. METHODS: Cultures taken between February and December 2002 were analysed. Blood cultures were processed using BacT/ALERT (Durham, NC), urine cultures by UROCULT (Bio-Bacter) and catheter tips in thioglycollate. BBL CRYSTAL identification system (BD, Sparks, MD) was used for identifying germs. Antibiotic sensitivity was determined by disk diffusion. RESULTS: There were 1,097 positive aerobic and facultative aerobic germ cultures; 64,3% were Gram-positive, 30,6% Gram-negative and 4,9% were yeasts. Gram-positive germs consisted of coagulase-negative staphylococci (64,2%), enterococcus (13,8%) and coagulase-positive staphylococci (13,3%). The most frequent Gram-negatives were Klebsiella (45,2%), Eschericha coli (30,9%) and Serratia (10,1%). Staphylococcus epidermidis accounted for 64% of the coagulase-negative staphylococci. S. epidermidis susceptibility to vancomycin was 100%. Coagulase-negative staphylococci susceptibility to rifampin and amikacin was 59% and 67,4% (respectively). Coagulase-negative staphylococci resistance to beta-lactams was 86,4% (95% CI: 82,3-89,9). Coagulase-positive staphylococci sensitivity to vancomycin was 100%. Gram-negative susceptibility to imipenem was 98,1% (95% CI: 89,9-99,9), 78,1% to gentamicin (95% CI: 64,9-88,2) and 46,6% to amikacin (95% CI: 28,3-65,7). CONCLUSIONS: There was high coagulase-negative staphylococci prevalence in neonatal infection (particularly S. epidermidis). All S. epidermidis and coagulase-positive staphylococci were sensitive to vancomycin. There was increasing coagulase-negative staphylococci and Gram-negative resistance to oxacillin and amikacin, respectively.

Aislamiento de Staphylococcus epidermidis portador de integrón clase 1 en un paciente con sepsis neonatal

Objetivo. Determinar la presencia y secuenciadel integron clase I en un aislamiento clinicode Staphylococcus epidermidis provenientede un neonato con diagnostico de sepsis.Materiales y metodos. En una cepa deS. epidermidis, aislada de una muestra dehemocultivo de un neonato, se realizaronlas pruebas de identificacion microbiologica,susceptibilidad antimicrobiana y la caracterizacionmolecular de los genes aac6´-aph2´´,mecA, el gen de la integrasa intI1 y el gencasete aac6´.

Determinación de valores de referencia para la microtécnica de fagocitosis y muerte intracelular de Cándida álbicans en un grupo de población sana en Bogotá

The function of the phagocytic cells is an efficient mechanism for non-specific protection against infectious agents and elimination of dead or senile cells. Cells of the innate immunity, such as neutrophils and macrophages, are responsible for this process. Neutrophils are particularly effective at the initial phases of infections caused by Gram negative and Gram positive bacteria. Macrophages participate in late stages of inflammation phagocyting bacteria and cellular debris. The techniques for evaluation of the phagocytic capacity are laborious, require large blood samples and need