Yong-Sub Yi

Biotransformation of eugenol to bis-eugenol by Kalopanax pictus cell culture

So far, the production of bis-eugenol has been conducted by chemical synthesis. However, because chemical synthesis of bis-eugenol has disadvantages such as regioselectivity and low yield, an environmentally friendly production of bis-eugenol by biological synthesis has become attractive. In the present study, bis-eugenol was produced using Kalopanax pictus callus culture. High-performance liquid chromatography analysis of reaction product revealed a new peak, whose structure was determined to be bis-eugenol by nuclear magnetic resonance spectroscopy. After 18 h of biotransformation using K. pictus cell, 16.3 mg/L of biseugenol was produced without any byproducts. This is the first report on production of bis-eugenol by biotransformation using plant cell culture.