Yoshifumi Fukunishi

Functional annotation of a full-length mouse cDNA collection

The RIKEN Mouse Gene Encyclopaedia Project, a systematic approach to determining the full coding potential of the mouse genome, involves collection and sequencing of full-length complementary DNAs and physical mapping of the corresponding genes to the mouse genome. We organized an international functional annotation meeting (FANTOM) to annotate the first 21,076 cDNAs to be analysed in this project. Here we describe the first RIKEN clone collection, which is one of the largest described for any organism. Analysis of these cDNAs extends known gene families and identifies new ones.The RIKEN Mouse Gene Encyclopaedia Project, a systematic approach to determining the full coding potential of the mouse genome, involves collection and sequencing of full-length complementary DNAs and physical mapping of the corresponding genes to the mouse genome. We organized an international functional annotation meeting (FANTOM) to annotate the first 21,076 cDNAs to be analysed in this project. Here we describe the first RIKEN clone collection, which is one of the largest described for any organism. Analysis of these cDNAs extends known gene families and identifies new ones.

Prediction of human cDNA from its homologous mouse full-length cDNA and human shotgun database.

We propose a prediction method for human full‐length cDNA by comparing sequence data between human genome shotgun sequence and mouse full‐length cDNA. The human genome which is homologous to the mouse full‐length cDNA is selected by a homology search program, and the predicted exons are connected at the exon‐intron junction which gives the best homology score to the mouse full‐length cDNA. The accuracy of the predicted human full‐length coding region is 83.3%, and the false positive rate is 16.7%. Five human full‐length proteins out of 20 proteins are correctly predicted.

Corrigendum to: Prediction of human cDNA from its homologous mouse full‐length cDNA and human shotgun database

Corrigendum to: Prediction of human cDNA from its homologous mouse full-length cDNA and human shotgun database [FEBS Letters 464 (1999) 129^132]1 Yoshifumi Fukunishia;b;*, Harukazu Suzukia, Masayasu Yoshinoa, Hideaki Konnoa;b, Yoshihide Hayashizakia;b aLaboratory for Genome Exploration Research Group, Genomic Sciences Center (GSC) and Genome Science Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan bCREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Corporation (JST), 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan