Young Gi Kim

Human immune response to a Pseudomonas aeruginosa outer membrane protein vaccine.

In order to evaluate in humans the safety and immunogenicity of a Pseudomonas aeruginosa vaccine composed of outer membrane proteins (OMPs), CFC-101, we carried out a phase I/IIa clinical trial in healthy male volunteers. Groups of six volunteers were immunized either subcutaneously (s.c.) or intramuscularly (i.m.) with three dosages of the vaccine three times at 7-day intervals. The vaccine was well tolerated by volunteers. Local reactions in the injection sites were generally mild and transient. Significant increases in OMP-specific antibody were observed in both route groups after vaccinations but was higher in the i.m.-immunized group, where vaccination with 0.5 or 1.0 mg doses yielded 100% seroconversion. The specificity of the induced antibodies to P. aeruginosa OMP was demonstrated by western blot analysis and immunoprecipitation assay. An increase in Clq-binding capacity and ability to confer mice protection from lethal challenges with P. aeruginosa indicated the protective efficacy of the elicited antibodies. Based on these data, we concluded that the P. aeruginosa OMP vaccine is safe and effective in humans with an optimal dose of 0.5 and 1.0 mg and that i.m. is the better route than s.c. for this vaccine.

Protection of mice against P. aeruginosa infections by large-scale affinity-purified human IgG specific to P. aeruginosa outer membrane proteins.

In order to develop an effective means to treat Pseudomonas aeruginosa infections, we designed a large-scale process for purification of human IgG specific to P. aeruginosa outer membrane proteins (Oprs) from normal human sera. The process we developed includes affinity column chromatography using P. aeruginosa Oprs as ligands, protein A column chromatography and ultrafiltration, which enriched P. aeruginosa Oprs-specific IgG antibody by 500-fold. The purified anti-Oprs IgG was specific to the Oprs as confirmed by an ELISA competition assay and retained opsonophagocytic-killing capacity. In vivo protective efficacy of anti-Oprs IgG was evaluated by passive protection assays in mice where the 50% protective dose of anti-Oprs IgG against P. aeruginosa infections was 41 microg/kg, which was 20 times lower than that of normal serum IgG. When administered to mice 3 h after bacterial challenge, only anti-Oprs IgG afforded protection. These data demonstrate the feasibility of use of the purification process in producing functionally active target-specific human antibodies for clinical use and provide a rationale for use of anti-Oprs IgG as a valuable adjunct to treat P. aeruginosa infections.

Development of 2D implicit particle simulation code for ohmic breakdown physics in a tokamak

Abstract A physical mechanism of an ohmic breakdown in a tokamak has not been clearly understood due to its complexity in physics and geometry especially for a role of space charge in the plasma. We have developed a 2D implicit particle simulation code BREAK, to study the ohmic breakdown physics under a realistic complicated situation considering the space charge and kinetic effects consistently. The ohmic breakdown phenomena span a broad range of spatio-temporal scales, from picoseconds order of the electron gyromotion to milliseconds order of the plasma transport. It is impossible to employ a typical explicit particle simulation method to see the slow plasma transport phenomena of our interest, because a time step size is restricted to be smaller than a period of the electron gyromotion in the explicit scheme. Hence, we adopt several physical and numerical models, such as a toroidally symmetric model and a direct-implicit method, to relax or remove the spatio-temporal restrictions. In addition, coalescence strategies are introduced to control the number of numerical super particles within acceptable ranges to handle the exponentially growing plasma density during the ohmic breakdown. The performance of BREAK is verified with several test cases so that BREAK is expected to be applicable to investigate the ohmic breakdown physics in the tokamak by considering 2-dimensional plasma physics in the RZ plane, self-consistently.

Evidence of a turbulent ExB mixing avalanche mechanism of gas breakdown in strongly magnetized systems

Although gas breakdown phenomena have been intensively studied over 100 years, the breakdown mechanism in a strongly magnetized system, such as tokamak, has been still obscured due to complex electromagnetic topologies. There has been a widespread misconception that the conventional breakdown model of the unmagnetized system can be directly applied to the strongly magnetized system. However, we found clear evidence that existing theories cannot explain the experimental results. Here, we demonstrate the underlying mechanism of gas breakdown in tokamaks, a turbulent ExB mixing avalanche, which systematically considers multi-dimensional plasma dynamics in the complex electromagnetic topology. This mechanism clearly elucidates the experiments by identifying crucial roles of self-electric fields produced by space-charge that decrease the plasma density growth rate and cause a dominant transport via ExB drifts. A comprehensive understanding of plasma dynamics in complex electromagnetic topology provides general design strategy for robust breakdown scenarios in a tokamak fusion reactor.Gas breakdown mechanism in plasma under the influence of complex electromagnetic field topology is still debatable. Here the authors present the evidence of the E×B mixing avalanche for gas breakdown in magnetized plasmas in fusion devices as tokamak.

Calibration of Thomson scattering system on VEST

The Thomson scattering system has been recently installed on Versatile Experiment Spherical Torus (VEST) to measure the electron temperature and the density of the core plasmas. Since the calibration of the system is required for the accurate measurement of these parameters, a polychromator and the system efficiency are calibrated. The bias voltage of the detector is optimized and the relative responsivity of the polychromator is measured to analyse the spectral broadening. The tendency of decreasing responsivity because of the ambient temperature change is addressed together. The efficiencies of the alignments using HeNe laser and Nd:YAG laser are compared. After the alignment using Rayleigh scattering, it is improved ~ 7 times while the peak signal of the stray light is decreased. To evaluate the efficiencies of the alignment using HeNe laser, it is compared with the efficiency of the fine alignment by Rayleigh scattering. After absolute calibration is done, the Thomson scattering signal is estimated theoretically. The Bayesian analysis is tried using the synthetic data, and the results show that the input temperature and the density are inside the contour of the 90% confident level. The calibrated Thomson scattering system will provide the meaningful information of the core plasma of the VEST.

Research of Fast DAQ system in KSTAR Thomson scattering diagnostic

The Thomson scattering diagnostic is one of the most important diagnostic systems in fusion plasma research. It provides reliable electron temperature and density profiles in magnetically confined plasma. A Q-switched Nd:YAG Thomson system was installed several years ago in KSTAR tokamak to measure the electron temperature and density profiles. For the KSTAR Thomson scattering system, a Charge-to-Digital Conversion (QDC) type data acquisition system was used to measure a pulse type Thomson signal. Recently, however, an error was found during the Te, ne calculation, because the QDC system had integrated the pulse Thomson signal that included a signal similar to stray light. To overcome such errors, we introduce a fast data acquisition (F-DAQ) system. To test this, we use CAEN V1742 5 GS/s, a Versa Module Eurocard Bus (VMEbus) type 12-bit switched capacitor digitizer with 32 channels. In this experiment, we compare the calculated Te results of Thomson scattering data measured simultaneously using QDC and F-DAQ. In the F-DAQ system, the shape of the pulse was restored by fitting.

Electron density profile measurements from hydrogen line intensity ratio method in Versatile Experiment Spherical Torus

Electron density profiles of versatile experiment spherical torus plasmas are measured by using a hydrogen line intensity ratio method. A fast-frame visible camera with appropriate bandpass filters is used to detect images of Balmer line intensities. The unique optical system makes it possible to take images of Hα and Hβ radiation simultaneously, with only one camera. The frame rate is 1000 fps and the spatial resolution of the system is about 0.5 cm. One-dimensional local emissivity profiles have been obtained from the toroidal line of sight with viewing dumps. An initial result for the electron density profile is presented and is in reasonable agreement with values measured by a triple Langmuir probe.