Yuemao Shen

Chemical and genetic diversity of Ligularia vellerea in Yunnan, China

Intra-specific diversity in Liularia vellerea growing in the northwestern to central Yunnan province of China was studied by chemical and genetic approaches. Samples collected in the Jianchuan, Lijiang, and Zhongdian areas contained 6,15-dioxygenated furanoeremophilanes as their major components (type A); whereas samples from the Luguhu area accumulated 1,6-dioxygenated furanoeremophilanes (type B); a sample from near Kunming, however, contained 6,15-dioxygenated eremophilanolides (type C). 11 beta H- and 11 alpha H-6 beta-angeloyloxy-15-carboxyeremophil-7-en-12,8-olides (eremofarfugins D and E) were also isolated and their structures were determined. A correlation between the composition and the DNA sequence was observed in the ITSs.

Two new cytotoxic C-21 steroidal glycosides from the root of Cynanchum auriculatum

Guided by in vitro antineoplastic tests, two new cytotoxic C-21 steroidal glycosides, auriculosides A (1) and B (2), were isolated from the root of Cynanchum auriculatum, Their structures were determined on the basis of chemical evidence and extensive spectroscopic methods including one-dimensional and two-dimensional NMR. These compounds showed cytotoxic activities against PC3, Hce-8693, Hela, and PAA cell lines

Chemical Constituents and Diversity of Ligularia lankongensis in Yunnan Province of China

The chemical constituents of the roots of Ligularia lankongensis collected at seven different places in Yunnan Province, China, were investigated together with the DNA sequence of the atpB- rbcL intergenic region. All the samples contained a new, highly oxygenated bisabolane-type sesquiterpene ( 1). Four other oxygenated bisabolanes ( 2 and the new 3, 4, and 5) were also obtained. Intraspecific diversity was observed in the composition of the compounds, but not in the DNA sequence.