Yukinori Hirota

Nucleotide sequence of the asnA gene coding for asparagine synthetase of E.coli K-12

We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin, ori, and asnA. The complete nucleotide sequence of the asnA gene was determined: the region of the structural gene extends 990 base-pairs at nucleotide positions 1434-2423 (see Fig. 3), which codes for a polypeptide of 330 amino-acid residues with a molecular weight of 36,688 daltons. The nucleotide sequences of the promoter and the ribosome-binding site of the gene are also assigned. We discuss the properties of its polypeptide.

MAPPING OF PROMOTERS IN THE REPLICATION ORIGIN REGION OF THE E. coli CHROMOSOME

ABSTRACT Promoters functioning in vivo in the region encompassing ori of the E. coli chromosome have been identified by using a promoter-cloning vecter pGA46 (An and Friesen: J. Bacteriol., 140, 400–407, 1979)( ori : defined as the minimal region carrying autonomously replicating function and spanning from positions 23 to 267 in our nucleotide coordinate (Oka et al.: Molec. Gen. Genet., 178, 9–20, 1980).