Yuqing Wan
Chinese Academy of Sciences
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Featured researches published by Yuqing Wan.
Biomaterials | 2003
Yuqing Wan; Jian Yang; Junlin Yang; Jianzhong Bei; Shenguo Wang
A series of gases were used for plasma treatment of poly-(L-lactide) (PLLA) under various conditions such as atmosphere, electric power, pressure and time. The NH(3) was preferably selected for modifying the surface of PLLA because it can obtain appropriate hydrophilicity and surface energy with high polar component compared to other gases. Subsequently, cells were seeded onto NH(3) modified surface and exposed to 29.5N/m(2) of shear stress field by means of a parallel plate flow chamber in order to get good insight into the influence of N-containing incorporation on cell retention, cell morphology, and cell shape factor. The results showed that cell retention on the modified PLLA was much higher than that on the unmodified one. The NH(3) plasma modified PLLA with high cell affinity and resistance to shear stress was gained. Surface hydrophilicity, surface energy with high polar component and N-containing groups may play an important role in enhancing cell resistance to shear stress. It revealed that the parallel plate flow chamber is an effective device for evaluating the effects of surface modification on the cell affinity of a material.
Biomaterials | 2003
Yuqing Wan; Wenna Chen; Jian Yang; Jianzhong Bei; Shenguo Wang
A series of poly(L-lactide)-poly(ethylene glycol) multiblock copolymers (Multi-PLE) with high molecular weight were synthesized and successfully used to fabricate three-dimensional scaffolds. Using mouse NIH 3T3 fibroblasts as model cells, the cell affinity of various Multi-PLE copolymers was evaluated and compared with that of poly(L-lactide) (PLLA) by means of cell attachment efficiency measurement, scanning electron microscopy observation and MTT assay. On one hand, the results showed that the cell attachment efficiency on Multi-PLE 4/1(4/1 refers to the molar ratio of lactidyl units to ethylene oxide units) films was close to that on PLLA film, however, the other Multi-PLE films exhibited much lower cell attachment efficiency than PLLA film, such as Multi-PLE 2/1 and Multi-PLE 1/1, which had higher PEG content. On the other hand, it was interesting to find that cell proliferation on Multi-PLE4/1 and Multi-PLE2/1 scaffolds was better than that on PLLA scaffold, which was closely related to the improved hydrophilicity of Multi-PLE copolymers due to the incorporation of PEG in comparison with pure PLLA. The Multi-PLE copolymer scaffolds with appropriate hydrophilicity were in favor of mass transportation, and then of cell proliferation and cell affinity. It meant that the cell proliferation would be much improved by increasing the hydrophilicity of the three-dimensional scaffolds, which even outweighed the disadvantages of the cell attachment efficiency reduction with the incorporation of PEG.
Biomaterials | 2003
Qing Cai; Yuqing Wan; Jianzhong Bei; Shenguo Wang
Brush-like biodegradable polylactide-grafted dextran copolymer (PLA-g-dextran) was by a bulk polymerization reaction using a trimethylsilyl-protected (TMS) dextran as macroinitiator and stannous octoate as catalyst. After the polymerization, the TMS groups could be easily removed by immersing the copolymer in methanol for 48 h. The PLA-g-dextran copolymers were characterized by (1)H NMR, GPC and intrinsic viscosity measurements. Besides, mouse 3T3 fibroblasts were cultured on these copolymeric substrates together with pure polylactide (PLA). Although the copolymers exhibited better hydrophilicity and cell affinity compared to pure PLA because of the incorporation of glucose units and the brush-like architecture, it was found that the cells still could not migrate into the center part of scaffold made of PLA-g-dextran copolymer. In result, PLA-g-dextran copolymers themselves were not an appropriate choice for the cell scaffold material, however, it could be used as compatilizer to ameliorate the compatibility between hydrophilic dextran and hydrophobic PLA due to its amphiphilic structure, which could improve the mechanical properties of PLA/dextran blends by reducing the phase separation between PLA and dextran. Therefore, the PLA/dextran blends, which had good cell affinity and moderate mechanical strength, might be prospect cell scaffold materials.
Chinese Science Bulletin | 2004
Shi-Zhao Kang; Yuqing Wan; Hui-Juan Yan; Jianzhong Bei; Chen Wang; Shenguo Wang; Chunru Wang; Li-Jun Wan; Chunli Bai
The composite material of poly-(L-lactide) (PLLA) and carbon nanotubes (CNTs) were prepared. Its surface morphologies and property were worked out by using atomic force microscopy (AFM) and contact angle measurement. Moreover, the prime cytocompatibility was used to investigate the biocompatibility of the composite material containing CNTs and the effects of CNTs on one aspect of cell function, cell affinity. The results obtained indicate that the composite material of PLLA and CNTs possesses good bio-compatibility for both the 3T3 fibroblasts and Oct-1 osteoblast-like cells. The addition of CNTs will greatly affect cell affinity of the material, which may be disadvantage for the cell adhesion.
Biomaterials | 2005
Yuqing Wan; Yong Wang; Zhimin Liu; Xue Qu; Buxing Han; Jianzhong Bei; Shenguo Wang
Biomaterials | 2004
Yuqing Wan; Xue Qu; Jun Lu; Chuanfeng Zhu; Li-Jun Wan; Junlin Yang; Jianzhong Bei; Shenguo Wang
Polymers for Advanced Technologies | 2003
Chifeng Tu; Qing Cai; Jian Yang; Yuqing Wan; Jianzhong Bei; Shenguo Wang
Polymer International | 2003
Jian Yang; Yuqing Wan; Chifeng Tu; Qing Cai; Jianzhong Bei; Shenguo Wang
Journal of Biomedical Materials Research Part A | 2003
Shenguo Wang; Qing Cai; Jianwei Hou; Jianzhong Bei; Tao Zhang; Jian Yang; Yuqing Wan
Biomaterials | 2006
Yuqing Wan; Chifeng Tu; Jian Yang; Jianzhong Bei; Shenguo Wang