Z. Tóth

Seed remains of common millet from the 4th (Mongolia) and 15th (Hungary) centuries: AFLP, SSR and mtDNA sequence recoveries

Gyulai, G., Humphreys, M. O., Lagler, R., Szabo, Z., Toth, Z., Bittsanszky, A., Gyulai, F., Heszky, L. (2006). Seed remains of common millet from the 4th (Mongolia) and 15th (Hungary) centuries: AFLP, SSR, and mtDNA sequence recoveries. Seed Science Research, 16, (3), 179-191. Keywords: ancient DNA, excavated seeds, Panicum miliaceum Sponsorship: Hungarian Scientific Research Group OTKA-M-04 563, Organization for Economic Co-operation and Development grant OECD-JA00018642, the USA–Hungarian Fulbright Commission, and a Sze´chenyi Fellowship (SzO- 4/2003).

RT-PCR analysis and stress response capacity of transgenic gshi-poplar clones (Populus x canescens) in response to paraquat exposure

Abstract Stress response capacity (Fv/Fm at 690 nm and F690/F735 at Fmax) of untransformed hybrid poplar, Populus × canescens (P. tremula × P. alba), and two transgenic lines overexpressing γ-ECS (γ-glutamylcysteine synthetase) either in the cytosol (cyt-ECS) or in the chloroplast (chl-ECS) was studied in response to the herbicide paraquat (4.0 × 10-9 to 4.0 × 10-6 m) for 21 days. Significant differences at sublethal (4.0 × 10-7 m) and bleaching (4.0 × 10-6 m) concentrations of paraquat were observed with about a two-fold and eight-fold decrease in the photosynthetic activity (Fv/Fm at 690 nm and F690/F735 at Fmax), respectively. None of the gshI transgenic lines (cyt-ECS, chl-ECS) with elevated GSH content exhibited significant tolerance to paraquat. Semiquantitative RT-PCR of the cyt-ECS clone was used for gene expression analysis of the nuclear encoded rbcS gene and the stress responsive gst gene. Expression of the constitutively expressed 26SrRNA ribosomal gene was probed as a control for all RT-PCR reactions. The relative intensities of gene expressions normalized to the level of 26SrRNA intensity showed a 50% decrease in the nuclear encoded rbcS expression and a 120% increase in the stress responsive gst gene expression of the paraquat treated (4.0 × 10-7 m) samples of the transgenic poplar line (cyt-ECS).