Zaixin Yang
University of Pennsylvania
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Publication
Featured researches published by Zaixin Yang.
Nature Biotechnology | 2004
Rebecca J. Morris; Yaping Liu; Lee Marles; Zaixin Yang; Carol S. Trempus; Shulan Li; Jamie S. Lin; Janet A Sawicki; George Cotsarelis
The hair follicle bulge possesses putative epithelial stem cells. Characterization of these cells has been hampered by the inability to target bulge cells genetically. Here, we use a Keratin1-15 (Krt1-15, also known as K15) promoter to target mouse bulge cells with an inducible Cre recombinase construct or with the gene encoding enhanced green fluorescent protein (EGFP), which allow for lineage analysis and for isolation of the cells. We show that bulge cells in adult mice generate all epithelial cell types within the intact follicle and hair during normal hair follicle cycling. After isolation, adult Krt1-15-EGFP-positive cells reconstituted all components of the cutaneous epithelium and had a higher proliferative potential than Krt1-15-EGFP-negative cells. Genetic profiling of hair follicle stem cells revealed several known and unknown receptors and signaling pathways important for maintaining the stem cell phenotype. Ultimately, these findings provide potential targets for the treatment of hair loss and other disorders of skin and hair.
Nature | 2007
Mayumi Ito; Zaixin Yang; Thomas Andl; Chunhua Cui; Noori Kim; Sarah E. Millar; George Cotsarelis
The mammalian hair follicle is a complex ‘mini-organ’ thought to form only during development; loss of an adult follicle is considered permanent. However, the possibility that hair follicles develop de novo following wounding was raised in studies on rabbits, mice and even humans fifty years ago. Subsequently, these observations were generally discounted because definitive evidence for follicular neogenesis was not presented. Here we show that, after wounding, hair follicles form de novo in genetically normal adult mice. The regenerated hair follicles establish a stem cell population, express known molecular markers of follicle differentiation, produce a hair shaft and progress through all stages of the hair follicle cycle. Lineage analysis demonstrated that the nascent follicles arise from epithelial cells outside of the hair follicle stem cell niche, suggesting that epidermal cells in the wound assume a hair follicle stem cell phenotype. Inhibition of Wnt signalling after re-epithelialization completely abrogates this wounding-induced folliculogenesis, whereas overexpression of Wnt ligand in the epidermis increases the number of regenerated hair follicles. These remarkable regenerative capabilities of the adult support the notion that wounding induces an embryonic phenotype in skin, and that this provides a window for manipulation of hair follicle neogenesis by Wnt proteins. These findings suggest treatments for wounds, hair loss and other degenerative skin disorders.
Science Translational Medicine | 2012
Luis A. Garza; Yaping Liu; Zaixin Yang; Brinda Alagesan; John A. Lawson; Scott M. Norberg; Dorothy E. Loy; Tailun Zhao; Hanz B. Blatt; David C. Stanton; Lee Carrasco; Gurpreet Ahluwalia; Susan M. Fischer; Garret A. FitzGerald; George Cotsarelis
Prostaglandin D2 inhibits hair growth through its receptor, GPR44, and this pathway could serve as a new target for developing treatments for male pattern baldness. A Not-So-Hairy Situation Everybody wishes his or her hair was different; curly hair wants straight locks, straight hair desires some curl. Patients with androgenetic alopecia (AGA), however, would take either one, as long as it meant having hair. AGA is a disorder that affects both men and women, leading to hair thinning and loss. Here, Garza and colleagues provide new insight into the pathogenesis of AGA, in hopes of developing new therapeutics that target specific pathways responsible for baldness. The authors first examined bald and haired scalp from five men with AGA and showed that the enzyme prostaglandin D2 (PGD2) synthase was elevated at the mRNA and protein levels in bald scalp only. In a larger group of 17 men, they confirmed that the synthase product PGD2 was also elevated in bald versus haired scalp. In mice with synchronized hair follicle cycling, Garza et al. uncovered a temporal relationship between PGD2 gene expression and hair follicle regression. The authors further found that PGD2 and a related metabolite, 15-dPGJ2, inhibited hair growth in both mice and human hair follicles, providing a crucial functional link between the prostaglandin pathway and AGA. Garza and coauthors identified the receptor GPR44 to be responsible for mediating the negative effects of PGD2. By discovering such therapeutic targets for downstream drug development, such as a topical treatment, Garza et al. may have given patients with AGA a long-awaited choice: curly or straight? Testosterone is necessary for the development of male pattern baldness, known as androgenetic alopecia (AGA); yet, the mechanisms for decreased hair growth in this disorder are unclear. We show that prostaglandin D2 synthase (PTGDS) is elevated at the mRNA and protein levels in bald scalp compared to haired scalp of men with AGA. The product of PTGDS enzyme activity, prostaglandin D2 (PGD2), is similarly elevated in bald scalp. During normal follicle cycling in mice, Ptgds and PGD2 levels increase immediately preceding the regression phase, suggesting an inhibitory effect on hair growth. We show that PGD2 inhibits hair growth in explanted human hair follicles and when applied topically to mice. Hair growth inhibition requires the PGD2 receptor G protein (heterotrimeric guanine nucleotide)–coupled receptor 44 (GPR44), but not the PGD2 receptor 1 (PTGDR). Furthermore, we find that a transgenic mouse, K14-Ptgs2, which targets prostaglandin-endoperoxide synthase 2 expression to the skin, demonstrates elevated levels of PGD2 in the skin and develops alopecia, follicular miniaturization, and sebaceous gland hyperplasia, which are all hallmarks of human AGA. These results define PGD2 as an inhibitor of hair growth in AGA and suggest the PGD2-GPR44 pathway as a potential target for treatment.
Nature Medicine | 2013
Ohsang Kwon; Zhikun Zhang; Michelle Spata; Maksim V. Plikus; Phillip D. Holler; Mayumi Ito; Zaixin Yang; Elsa Treffeisen; Chang D Kim; Arben Nace; Xiaohong Zhang; Sheena Baratono; Fen Wang; David M. Ornitz; Sarah E. Millar; George Cotsarelis
Understanding molecular mechanisms for regeneration of hair follicles provides new opportunities for developing treatments for hair loss and other skin disorders. Here we show that fibroblast growth factor 9 (Fgf9), initially secreted by γδ T cells, modulates hair follicle regeneration after wounding the skin of adult mice. Reducing Fgf9 expression decreases this wound-induced hair neogenesis (WIHN). Conversely, overexpression of Fgf9 results in a two- to threefold increase in the number of neogenic hair follicles. We found that Fgf9 from γδ T cells triggers Wnt expression and subsequent Wnt activation in wound fibroblasts. Through a unique feedback mechanism, activated fibroblasts then express Fgf9, thus amplifying Wnt activity throughout the wound dermis during a crucial phase of skin regeneration. Notably, humans lack a robust population of resident dermal γδ T cells, potentially explaining their inability to regenerate hair after wounding. These findings highlight the essential relationship between the immune system and tissue regeneration. The importance of Fgf9 in hair follicle regeneration suggests that it could be used therapeutically in humans.
Journal of Gene Medicine | 2001
Abha R. Gupta; Nadine S. Dejneka; Robert J. D'Amato; Zaixin Yang; Nasreen A. Syed; Albert M. Maguire; Jean Bennett
A promising strategy for delaying death of photoreceptor cells in retinal degenerative disease is to support survival of these cells through intraocular delivery of growth/neurotrophic factors. One factor that has received great attention is basic fibroblast growth factor (bFGF; fgf‐2), a known stimulator of angiogenesis. We evaluated the potential for neovascularization induced by adenovirus‐mediated intravitreal delivery of bFGF.
Nature Medicine | 2005
Mayumi Ito; Yaping Liu; Zaixin Yang; Jane Nguyen; Fan Liang; Rebecca J. Morris; George Cotsarelis
Journal of Investigative Dermatology | 2003
Yaping Liu; Stephen Lyle; Zaixin Yang; George Cotsarelis
Molecular Therapy | 2002
Vibha Anand; Bethany Duffy; Zaixin Yang; Nadine S. Dejneka; Albert M. Maguire; Jean Bennett
Molecular Therapy | 2008
Masayuki Endo; Philip W. Zoltick; William H. Peranteau; Antoneta Radu; Nidal Muvarak; Mayumi Ito; Zaixin Yang; George Cotsarelis; Alan W. Flake
Experimental Dermatology | 2008
Mayumi Ito; Yaping Liu; Zaixin Yang; Jane Nguyen; R. Morris; Fan Liang; George Cotsarelis