Zeynep Mustafaeva

Synthesis of microwave-assisted poly(methyl vinyl ether-co-maleic anhydride)-bovine serum albumin bioconjugates

Abstract The water-soluble poly(methyl vinyl ether-co-maleic anhydride) copolymer-bovine serum albumin bioconjugates were synthesized in the presence of 1-ethyl-3-(3-dimetilamino-propyl) carbodiimide hydrochloride as cross-linking agents via microwave-assisted and conventional methods and characterized by size-exclusion chromatography and high-performance liquid chromatography. According to size-exclusion chromatography and high-performance liquid chromatography results, the bioconjugates synthesized in the microwave-assisted method are more stable and efficient than the conventional method. The reaction time is shortened from 17 hours to 15 minutes by means of the microwave-assisted method.

The Fluorescence Study of Interaction Between Bovine Serum Albumin and Polyacrylic Acid

The complex formation of Bovine Serum Albumin (BSA) with anionic polyelectrolyte (polyacrylic acid, PAA) in aqueous solution was studied by a fluorescence technique, pH titration and HPLC analysis. The character of the interactions and solubility of the polycomplex particles depends on the BSA/PAA ratios and the pH of solution. The interaction at pH > pI (isoelectric point of BSA) (pH 6.0–7.0) is negligible weak and at pH 5.0 results with the formation of stable water-soluble polycomplexes at a wide range of protein/polymer ratios. The fluorescence intensity of BSA sharply decreased when an different amount of PAA was added and its maximum wavelength shifts towards the blue region. The protein molecules in the structure of soluble polycomplex particles are densely covered by the shelf of a polymer coil and practically “fenced off” from the water environment. This effect was reinforced by the increase of protein components. Existence of soluble and insoluble PAA-BSA complexes have been observed at pH < pI (pH 4.0–4.3). These soluble complexes characterized by the structure of particles in which protein molecules are densely covered by the shelf of a polymer coil. By the increase in the protein concentration, these complexes aggregate to an interpolymer species.

Investigation of metal–polyelectrolyte complex toxicity

Water-soluble binary and ternary copper complexes of polyelectrolytes were synthesized, and the toxicity of these complexes was tested in mouse fibroblast cell line (L929) in vitro. Both the binary and ternary complexes were prepared at the ratio of 0.4 mole copper(II) ions per monomer of acrylic acid and 0.5 mole copper(II) ions per monomer of methyl vinyl ether maleic anhydride, furthermore at the ratio of 1 and 2 mole bovine serum albumin per mole of polyacrylic acid and poly(methyl vinyl ether-co-maleic anhydride), respectively. Compared to binary copper(II)–polyelectrolyte complexes, these ternary complexes have been determined to be of least toxicity.

The Formation of Polycomplexes of Poly(Methyl Vinyl Ether-Co-Maleic Anhydride) and Bovine Serum Albumin in the Presence of Copper Ions

Abstract The binary and ternary complex formations of poly(methyl vinyl ether-co-maleic anhydride) (PMVEMA) with copper ions and with bovine serum albumin (BSA) in the presence of copper ions in phosphate buffer solution at pH = 7 were examined by the techniques of UV-visible, fluorescence, dynamic light scattering, atomic force microscopy measurements. In the formation of binary complexes of PMVEMA-Cu(II), the addition of copper ions to the solution of PMVEMA in phosphate buffer solution at pH = 7 forms homogeneous solutions when the molar ratio of Cu(II)/MVEMA is 0.5. Then the formations of ternary complexes of PMVEMA-Cu(II)-BSA were examined. Study analysis revealed that the toxicities of polymer-metal and polymer-metal-protein mixture solutions depend on the nature and ratio of components in mixtures.