Zhihao Dong

Effects of four short-chain fatty acids or salts on the dynamics of nitrogen transformations and intrinsic protease activity of alfalfa silage

BACKGROUND Short-chain fatty salts have been widely used as food and forage preservatives because of their antimicrobial properties. This study evaluated the effects of four chemical compounds with antimicrobial properties on nitrogen transformations and intrinsic protease activity of alfalfa silage. RESULTS Potassium diformate (PD) and formic acid (FA) rapidly reduced silage pH. Silages treated with sodium diacetate (SD) and calcium propionate (CAP) had higher final peptide N concentrations than other silage. The free amino acid N contents in PD and FA treated silages were lower than other silages at all intervals of ensilage. The ammonia N concentrations in FA and PD silages were the lowest, followed by SD and CAP silages. As ensiling progressed, the aminopeptidase activity was completely lost by day 5 for FA and PD silages and inactive by day 7 for SD silage, while it remained active after day 7 for control and CAP silage. The carboxypeptidase activities in FA and PD silages were already reduced below 50% by day 1 of ensiling. CONCLUSION Potassium diformate was as effective as formic acid in depressing the proteolysis, while sodium diacetate and calcium propionate were inferior to formic acid in protecting alfalfa proteins from being hydrolysed.

Characterization of Enterococcus faecalis JF85 and Enterococcus faecium Y83 isolated from Tibetan yak (Bos grunniens) for ensiling Pennisetum sinese

Two bacteria strains with cellulolytic potential isolated from Tibetan yak (Bos grunniens) rumen were identified as Enterococcus faecalis (JF85) and Enterococcus faecium (Y83). Isolates grow well within a range of temperature 15 to 55 °C and pH 3.0-7.0, respectively. Two strains were inoculated with or without Lactobacillus plantarum (Lp) to Pennisetum sinese silage for 90 days. All inoculants increased lactic acid content, decreased pH and lignocellulose contents compared with silage without additives (control). The lowest pH, highest lactic acid and largest reduction in lignocellulose contents were observed in JF85+Lp and Y83+Lp silages. Isolates alone or in combination with Lp significantly increased WSC, mono- and disaccharides contents as compared to the control. Combined addition efficiently improved enzymatic hydrolysis of Pennisetum sinese silage, indicated by higher glucose yield and cellulose convertibility. Pennisetum sinese ensiled with combined additives is a suitable storage and pretreatment method prior to sugars production from energy crop.

Characteristics of isolated lactic acid bacteria and their effects on the silage quality

Objective Four lactic acid bacteria (LAB) strains isolated from common vetch, tall fescue and perennial ryegrass on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silage were studied. Methods The four isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD-1) were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. The five LAB strains were added to Italian ryegrass for ensiling at three different temperatures (10°C, 15°C, and 25°C). Results All isolated strains (LCG3, LTG7, I5, and LI3) could grow at 5°C to 20°C, pH 3.0 to 8.0 and NaCl (3.0%, 6.5%). Strains LCG3, LTG7, I5, and LI3 were identified as Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, and Lactobacillus casei by sequencing 16S rDNA, respectively. All LAB inoculants significantly (p<0.05) increased lactic acid (LA) contents and ratios of lactic acid to acetic acid, and reduced pH and ammonia nitrogen/total nitrogen (AN/TN) compared with uninoculated silages at various temperatures (10°C, 15°C, and 25°C). Compared to the commercial inoculant G, I5, and LI3 showed similar effects on improving the silage quality of Italian ryegrass at 10°C and 15°C, indicated by similar pH, LA content and AN/TN. Conclusion All inoculants could improve the silage fermentation quality at various temperatures (10°C, 15°C, and 25°C). At the temperature of 10°C and 15°C, strain I5 and LI3 had similar effects with the commercial inoculant G on improving the silage quality of Italian ryegrass.

Effects of four short-chain fatty acids or salts on fermentation characteristics and aerobic stability of alfalfa (Medicago sativa L.) silage

BACKGROUND The objective of the present study was to evaluate the effects of four chemicals on the fermentation quality and aerobic stability of alfalfa (Medicago sativa L.) silage. Wilted alfalfa was ensiled without additive (control), or with formic acid (FA), potassium diformate (KDF), sodium diacetate (SDA) or calcium propionate (CAP). RESULTS After 60 days of ensiling, the pH values in FA, KDF and SDA silages were lower (P < 0.05) compared to that of control and CAP silages, and chemicals (P < 0.05) decreased butyric acid and ammonia N concentrations and populations of aerobic bacteria and yeasts compared to the control. The SDA and CAP silages had a higher (P < 0.05) lactic acid bacteria content compared to the FA and KDF silages. The SDA and CAP silages had higher (P < 0.05) acetic and propionic acid contents compared to the other silages, respectively. The ammonia N concentrations in the FA and KDF silages were lower compared to the other silages during the first 5 days of aerobic exposure, and then increased sharply to 105 and 100 g kg-1 total N, respectively, which was higher (P < 0.05) than that of the SDA and CAP silages on day 9 of aerobic exposure. Yeasts and aerobic bacteria counts in SDA silage slowly increased and remained at lower levels compared to the other silages after 7 days of aerobic exposure. CONCLUSION Additives prolonged the aerobic stability duration compared to the control, and the SDA and CAP silages remained stable for more than 216 h, followed by the KDF and FA silages (202 and 196 h, respectively).

Phylogenetic identificationI of lactic acid bacteria isolates and their effects on the fermentation quality of sweet sorghum (sorghum bicolor) silage

To isolate, screen and identify the lactic acid bacteria (LAB) from elephant silage during the process of the fermentation and their effects on the fermentation quality of sweet sorghum silage.

Effects of storage temperature and combined microbial inoculants on fermentation end products and microbial populations of Italian ryegrass (Lolium multiflorum Lam.) silage

To examine five lactic acid bacteria (LAB) strains (LCG9, LTG7, I5, TG1 and LI3) isolated from the Tibetan Plateau, and evaluate their combined effects on the silage quality of Italian ryegrass (Lolium multiflorum Lam.) at three temperatures (10, 15 and 25°C).

Ensiling as pretreatment of rice straw: The effect of hemicellulase and Lactobacillus plantarum on hemicellulose degradation and cellulose conversion

The fermentation characteristics, structural carbohydrate degradation and enzymatic hydrolysis of rice straw ensiled with hemicellulase and Lactobacillus plantarum were examined. Fresh rice straw was ensiled in 1-L laboratory silos with no additive control (CK), L. plantarum (L), hemicellulase (HC) and hemicellulase + L. plantarum (HCL) for 6, 15, 30 and 60 days. All additives increased lactic acid concentration, and reduced pH and lignocellulosic content of the resulting silage relative to the control. The highest organic acid and residual sugar contents and lignocellulose degradation were observed in HCL silage. Hemicellulase alone or combined with L. plantarum improved the enzymatic hydrolysis with higher glucose yield and cellulose convertibility. Fresh rice straw ensiled with the combined additives increased feedstock preservation and cellulose conversion, and is thus recommended as a biological pretreatment for subsequent biofuel production.

The effects of fibrolytic enzymes, cellulolytic fungi and bacteria on the fermentation characteristics, structural carbohydrates degradation, and enzymatic conversion yields of Pennisetum sinese silage

Biological inoculants were tested on Pennisetum sinese for their effects on fermentation characteristics, structural carbohydrates degradation, and enzymatic conversion yields. Pennisetum sinese was ensiled without additive, Lactobacillus plantarum (Lp), Trichoderma reesei (Tr), fibrolytic enzymes (E), and Enterococcus faecium (Y83) for 90 days. Y83 silages had higher LA and lower AA, ammonia-N and DM loss as compared to E and Tr silages. Tr and E had superior effects for degrading lignocellulose while Y83 had intermediate effects. The first-order exponential decay models (R2 = 0.928-0.998) predicted nonstructural carbohydrates kinetics and demonstrated high water soluble carbohydrate (g/kg DM) preservation potential in Y83 (21.40), followed by Tr (18.94) and E (16.74). Addition of Y83 improved the conversion efficiency of P. sinese silage than Tr and E, indicated by higher glucose and total reducing sugars yield (22.49 and 36.89 w/w % DM, respectively). In conclusion, Y83 can be exploited for the ensiling lignocellulosic biomass before grass processing.

Effects of applying lactic acid bacteria and propionic acid on fermentation quality, aerobic stability and in vitro gas production of forage-based total mixed ration silage in Tibet

Total mixed ration (TMR) silage technology has been practically used to feed ruminants in Tibet. This study was conducted on forage-based TMR to evaluate the effects of supplementing lactic acid bacteria and propionic acid on its fermentation characteristics, aerobic stability and in vitro gas production kinetics and digestibility. Experimental treatments included four variants: (1) TMR supplemented with 10 mL deionised water per kilogram fresh matter (Control); (2) TMR supplemented with 1 × 106 cfu/g Lactobacillus plantarum (L); (3) TMR supplemented with 0.3% propionic acid (P); (4) TMR supplemented with a combination of 1 × 106 cfu/g Lactobacillus plantarum and 0.3% propionic acid (LP). The latter three additives were first dissolved in deionised water and then applied as a water solution (10 mL/kg fresh matter). All treatments were ensiled in laboratory-scale silos for 45 days, and then exposed to air for 12 days to evaluate the aerobic stability of TMR silage. Further, the four experimental treatments were fermented with buffered rumen fluid to measure in vitro gas production and nutrients’ digestibility. The results indicated that all TMR silages possessed good fermentation characteristics with low pH values ( 66 g/kg DM) and Flieg points (>80). The addition of L and LP stimulated a more efficient homofermentation of TMR silage than in the variant without L, as evidenced by higher ratios of lactic : acetic acid. The addition of P had no effect (P > 0.05) on lactic acid production of TMR silage compared with the Control, whereas it decreased NH3-N content (P < 0.05). Under aerobic conditions, L silage showed less aerobic stability compared with the Control silage, whereas P and LP silages were more (P < 0.05) aerobically stable. Compared with the Control, all additives elevated (P < 0.05) the total gas production and in vitro dry matter digestibility of TMR silages. L silage had a higher (P < 0.05) in vitro neutral detergent fibre digestibility than the Control silage. Data obtained from this study suggested that TMR silage based on oat and common vetch can be well conserved with or without additives. Lactic acid bacteria were compatible with propionic acid, and addition of lactic acid bacteria together with propionic acid can improve the fermentation quality, aerobic stability and in vitro dry matter digestibility of TMR silage.

Effect of lactic acid bacteria and propionic acid on conservation characteristics, aerobic stability and in vitro gas production kinetics and digestibility of whole-crop corn based total mixed ration silage

Abstract This study was conducted to evaluate the effect of lactic acid bacteria and propionic acid on the fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility of whole-crop corn based total mixed ration (TMR) silage. Total mixed ration was ensiled with four treatments: (1) no additives (control); (2) an inoculant (Lactobacillus plantarum) (L); (3) propionic acid (P); (4) propionic acid+lactic acid bacteria (PL). All treatments were ensiled in laboratory-scale silos for 45 days, and then subjected to an aerobic stability test for 12 days. Further, four TMR silages were incubated in vitro with buffered rumen fluid to study in vitro gas production kinetics and digestibility. The results indicated that all TMR silages had good fermentation characteristics with low pH (