Zhongbao Li

Isolation and characterization of ten microsatellite markers of Fenneropenaeus penicillatus

Ten polymorphic microsatellite loci in Fenneropenaeus penicillatus were isolated and characterized. Microsatellite polymorphism was tested by using 30 individuals from one wild population. The results showed that the number of alleles per locus and the polymorphism Information Content ranging from 3 to 7 and from 0.1813 to 0.6087, respectively. The observed and expected heterozygosity were 0.1379–0.2727 and 0.1308–0.5597, respectively. The loci identified in this study could provide a useful tool for ongoing population genetic structure analysis of F. penicillatus.

Isolation and characterization of novel microsatellite markers for molecular genetic diversity in Siganus fuscescens.

The rabbitfish Siganus fuscescens is an economically valuable species that is widely distributed throughout the estuaries, intertidal, and offshore coasts of the Indo-Pacific and eastern Mediterranean. Ten novel microsatellite loci from the genome of S. fuscescens were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats. Polymorphisms in these 10 microsatellite markers were determined from 32 wild individuals. The number of alleles per locus and the polymorphism information content ranged from 2 to 5 and from 0.059 to 0.668, respectively. The observed and expected heterozygosities varied from 0.063 to 0.781 and from 0.062 to 0.731, respectively. Although 1 locus (LZY-X7, P < 0.005) showed significant deviation from the Hardy-Weinberg equilibrium, no deviations were detected in the other 9 loci. These microsatellite loci may be useful for further population genetic studies, conservation studies, population structure assessment, and linkage map construction of S. fuscescens.

Isolation and characterization of eleven microsatellite loci in the marbled rockfish, Sebastiscus marmoratus (Scorpaenidae)

The marbled rockfish, Sebastiscus marmoratus, is an important commercially near-shore fish inhabiting littoral rocky bottoms from Japan to the East China Sea. Eleven polymorphic microsatellite loci were isolated from S. marmoratus and the polymorphism of these loci was observed in 32 samples from one wild population. The number of alleles ranged from 2 to 8. The polymorphism information content ranged from 0.155 to 0.752. The levels of the observed and expected heterozygosity were 0.186–0.969 and 0.170–0.782, respectively. Only one locus (Sm3-63) deviated from Hardy–Weinberg equilibrium. These microsatellite markers can provide a useful tool for further study on genetic diversity and conservation of S. marmoratus.

Screening and characterization of new microsatellite markers in Fenneropenaeus penicillatus.

Fenneropenaeus penicillatus, with high protein and low fat, is a commercially important aquatic product in China. Microsatellite loci were developed according to the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats. Eight new polymorphic microsatellite markers for F. penicillatus were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The polymorphism information content ranged from 0.2703 to 0.7598, and the number of alleles per locus varied from 3 to 6. The observed and expected heterozygosities were 0.1613-0.5556 and 0.2347-0.7387, respectively. No significant deviations from Hardy-Weinberg equilibrium (P > 0.00625) were detected. These polymorphic microsatellite loci will be useful to study the genetic diversity and population structure of F. penicillatus.

Characterization of eight novel microsatellite markers in the green-lipped mussel Perna viridis (Mytilidae).

The green lipped mussel, also known as the Asian green mussel (Perna viridis) is a fast reproducing and valuable food source, but it is also considered an invasive species and can clog and damage pipes and marine equipment. Eight novel polymorphic microsatellite loci for P. viridis were isolated and characterized. Microsatellite polymorphism was evaluated in 30 individuals collected from Xiamen, China. The number of alleles per locus and the polymorphism information content ranged from 2 to 5 and from 0.3092 to 0.7031, respectively. The observed and expected heterozygosities were 0.1538-0.8400 and 0.1448-0.6833, respectively. The loci identified in this study could provide a useful tool for the genetic population structure analysis of P. viridis.

Isolation and characterization of twelve novel polymorphic microsatellite loci in the Branchiostoma belcheri Gray (Amphioxus)

Branchiostoma belcheri Gray is an endangered species. A set of twelve novel polymorphic microsatellite loci was developed in B. belcheri Gray. The polymorphism of these loci was tested on a population of thirty individuals from Xiamen, China. The number of alleles ranged from 3 to 7. The polymorphism information content ranged from 0.309 to 0.778. The levels of expected and observed heterozygosities varied from 0.2449 to 0.7825, and from 0.1786 to 0.8571, respectively. Three loci (WCY4-22, WCY4-39, WCY5-17) deviated from Hardy–Weinberg equilibrium. These microsatellite loci would contribute to population genetic studies and conservation of B. belcheri Gray.

Development and characterization of microsatellite loci for Fenneropenaeus penicillatus Alcock

Eight novel microsatellite loci from the genome of Fenneropenaeus penicillatus Alcock were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Thirty (30) wild individuals were used to analyze the polymorphism of these eight microsatellite markers. The results show that the number of alleles per locus and the polymorphism information content ranged from 2 to 7 and from 0.2076 to 0.7484, respectively. The observed and expected heterozygosity were 0.1724 to 0.9130 and 0.1639 to 0.7314, respectively. These microsatellite primers will be used for further population genetic studies, constructing genetic linkage maps or locating quantitative trait locus (QTL) of F. penicillatus Alcock. Keywords: Genetic markers, Fenneropenaeus penicillatus Alcock, microsatellites

Characterization of new microsatellite markers of Siganus fuscescens (Siganidae).

Siganus fuscescens, which is a small commercially important marine fish, is wildly distributed in shallow waters throughout the tropical and subtropical Indo-Pacific and Eastern Mediterranean regions. It is part of a group known as rabbitfish. Fifteen new polymorphic microsatellite markers for S. fuscescens were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 2 to 12, and the polymorphism information content ranged from 0.210 to 0.849. The observed and expected heterozygosities were 0.142-0.808 and 0.225-0.853, respectively. Although significant deviations from Hardy-Weinberg equilibrium were detected at 2 loci (Sf1-37-2 and Sf1-47), no significant deviations were detected at the other 13 loci. These microsatellite markers will provide a useful tool for studies on genetic diversity and differentiation of S. fuscescens.

Loss of Genetic Diversity in Hatchery Produced Haliotis Diversicolor Supertexta by AFLP

Information on genetic diversity and differentiation is essential for conservation and stock improvement programs. AFLP technique was applied to analyze the genetic diversity and differentiation of one wild and three hatchery Haliotis diversicolor supertexta populations. In general, our results showed a reduced genetic diversity in the three hatchery populations when compared with the one wild population. The results showed that in three hatchery populations, the percentage of polymorphic loci ranged from 42.08% to 51.91%, the mean number of alleles per locus ranged from 1.4208 to 1.5191, the mean effective number of alleles per locus ranged from 1.2790 to 1.3547, Nei’s gene diversity ranged from 0.1633 to 0.2044, and the Shannon’s information index ranged from 0.2414 to 0.3002. While in the wild population, the percentage of polymorphic loci was 61.48%, the mean number of alleles per locus was 1.6148, the mean effective number of alleles per locus was 1.4233, Nei’s gene diversity was 0.2450, and the Shannon’s information index was 0.3598. The genetic differentiation among the four populations was low. The genetic distance between the wild NA population and other three hatchery populations ranged from 0.0372 to 0.0556.

The Genetic Diversity and Differentiation of Haliotis Ovina by AFLP

Amplified fragment length polymorphism technique has been applied to detect the genetic diversity and differentiation of three wild Haliotis ovina populations in Hainan province, China. High level of genetic diversity was detected among the populations. The mean effective number of alleles per locus was from 1.2027+0.3090 to 1.3530+0.3404, with an average of 1.2969+0.3338. The percentage of polymorphic loci ranged from 66.54% to 60.36%, with an average of 76.18%. The mean of Shannons Information index was 0.2870+0.2422, ranging from 0.2026+0.2403 to 0.3397+0.2440. And the average of gene diversity index was 0.1981+0.1767, ranging from 0.1306+0.1686 to 0.2276+0.1791. In general, the genetic differentiation among the three populations was insignificant, the average genetic distance among the three populations was 0.0201, ranging from 0.0087 to 0.0462. The UPGMA dendrogram showed that Yinzhou and Yalongwan first clustered into a group and then grouped with Anyou.