Zhongkun Du

Toxic effects of 1-decyl-3-methylimidazolium bromide ionic liquid on the antioxidant enzyme system and DNA in zebrafish (Danio rerio) livers

Ionic liquids were recently found to be toxic to aquatic organisms. Therefore, the present study investigated the effects of 1-decyl-3-methylimidazolium bromide ([C10mim]Br) on oxidative stress and DNA damage in zebrafish. Male and female zebrafish were separated and exposed to five concentrations of [C10mim]Br (0, 5, 10, 20, and 40 mg L(-1)) and were sampled on days 7, 14, 21 and 28. Compared to control groups, the activities of antioxidant enzymes were significantly decreased at most exposure intervals. This decreased activity resulted in the production of excess reactive oxygen species (ROS) and increased malondialdehyde (MDA) content in zebrafish liver. Additionally, it was noteworthy that a clear dose-response was found for DNA damage. As for sex differences, significant differences in catalase (CAT) and ROS were found on the 7th day. In conclusion, the exposure of [C10mim]Br caused DNA damage, leading to antioxidant responses in zebrafish livers.

Effects of the ionic liquid (Omim)PF6 on antioxidant enzyme systems, ROS and DNA damage in zebrafish (Danio rerio)

The present study examined the toxic effects of the exposure to different concentrations of the ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate for a 28-day period in zebrafish (Danio rerio) (sampled at 7, 14, 21 and 28 d). The activities of antioxidant enzymes (superoxide dismutase and catalase), levels of reactive oxygen species and DNA damage in fish livers served as the indicators to assess the toxicity of [Omim]PF(6) to zebrafish. The ionic liquid inhibited the activities of the antioxidant enzymes and caused the accumulation of ROS and DNA damage, and the results were concentration- and time-dependent. Male and female fish were tested separately and no differences were observed. The results showed that the ionic liquid could induce oxidative stress and DNA damage in zebrafish and that these effects could also accumulate over time.

Acute and chronic toxic effects of fluoxastrobin on zebrafish (Danio rerio)

Fluoxastrobin is a new strobilurin fungicide, similar to azoxystrobin and pyraclostrobin. Before the wide application of fluoxastrobin, the present study was performed to assay the acute and chronic toxicity of fluoxastrobin on zebrafish (Danio rerio). The 96-hour median lethal concentration (96h LC50) after initiation of zebrafish exposure to fluoxastrobin was 0.51mg/L with a 95% confidence interval of 0.45 to 0.57mg/L, indicating that fluoxastrobin was highly toxic to zebrafish. As endpoints, we assayed the levels of reactive oxygen species (ROS), malondialdehyde (MDA), the activities of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and the degree of DNA damage at three different doses, 0.001, 0.01, and 0.1mg/L on days 7, 14, 21, and 28. The antioxidant enzymes partially ameliorated the ROS induced by fluoxastrobin t and were in turn inhibited by excess ROS, especially at 0.1mg/L. Lipid peroxidation and DNA damage were stimulated by ROS. The fluoxastrobin contents of the tested solutions were also determined; at the fluoxastrobin doses of 0.001, 0.01, and 0.1mg/L, the contents on day 28 were 3.9, 5.0, and 0.64% greater than those on day 0. Thus, fluoxastrobin was relatively stable in an aquatic environment. In addition, the present study provided more information regarding the toxic effects of fluoxastrobin and the scientific methods for selection and evaluation of fungicides in the future.

Effects of 1-octyl-3-methylimidazolium nitrate on the microbes in brown soil

The toxicity of ionic liquids (ILs) on soil organisms has aroused wide attention due to their high-solubility. The present investigation focused on the toxicity of 1-octyl-3-methylimidazolium nitrate ([C8mim]NO3) on the microbial populations (bacteria, fungi, and actinomycetes), soil enzyme (urease, dehydrogenase, acid phosphatase, and β-glucosidase) activities, microbial community diversity using terminal restriction fragment length polymorphism (T-RFLP), and abundance of the ammonia monooxygenase (amoA) genes of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) using quantitative real-time polymerase chain reaction (q-PCR) in brown soil at each trial with doses of 0, 1.0, 5.0, and 10.0mg/kg on days 10, 20, 30, and 40. The contents of [C8mim]NO3 in soil were measured using high performance liquid chromatography with recoveries of 84.3% to 85.2%, and changed less than 10% during the experimental period. A significant decrease was observed from the bacteria, fungi and actinomycetes populations at 10.0mg/kg, at which the urease activity was inhibited and the β-glucosidase activity was stimulated on days 20, 30, and 40. In addition, [C8mim]NO3 inhibited the dehydrogenase activity at 10mg/kg on days 30 and 40 and the acid phosphatase activity on day 20. The diversity of the soil microbial community and the gene abundance of AOA- and AOB- amoA were also inhibited. Furthermore, the present investigation provided more scientific information for the toxicity evaluation of ILs in soil.

Toxic effect of [Omim]BF 4 and [Omim]Br on antioxidant stress and oxidative damage in earthworms ( Eisenia fetida )

In this paper, model soil organism, earthworms (Eisenia fetida), were selected to examine the chronic toxic effect of two different ionic liquids (ILs) [Omim]BF4 and [Omim]Br. Earthworms were put into different ILs concentrations (0, 5, 10, 20, 40 mg/kg) in artificial soil and random selected on days 7, 14, 21 and 28. Reactive oxygen species (ROS), antioxidant enzymes and detoxifying enzyme glutathione-S-transferase (GST) were researched for determination of antioxidant stress. Malondialdehyde (MDA) and olive tail moment (OTM) were researched to determine the oxidative damage. Both the pollutants had the same effect on earthworms: ILs led to accumulation of ROS, and then antioxidant enzymes and detoxification enzyme all changed to eliminate the effects of ROS, and the above process led to lipid peroxidation and DNA damage in earthworms. This paper shows that [Omim]BF4 and [Omim]Br both caused toxicity to earthworms and had the similar toxicity levels.

Determination of residual concentration of ionic liquids with different anions and alkyl-chain lengths in water and soil samples

Considering the wide synthesis and application of ionic liquids (ILs), the toxicity of ILs has recently gained growing attention. However, few studies focused on IL determination methods in environmental samples. In the present study, we implemented the determination methods for the 12 ILs with different chemical structures using high-performance liquid chromatography (HPLC) and ultraviolet (UV) spectrophotometry. The optimum conditions for extraction of ILs from soil samples were also obtained by single-factor experiments and response surface methodology (RSM). The instrument detection limits (IDLs) reached 10-10 g. Compared to the use of UV, HPLC had the standard curve with stronger correlation (R2 ≥ 0.999) and lower detection limit. We therefore used HPLC to detect the contents of ILs in water and soil samples. A standard adding method was used for the reliability test of the above methods. The average recovery in water samples was 90.46%-108.83%, and the coefficient of variation (CV) was 0.51%-9.07%. The method detection limits (MDLs) were below 0.1 mg/L. The optimized IL extraction conditions in soil samples were as follows: The ratio of methanol and saturated ammonium chloride was 90:10, the ultrasonic time was 50 min, and the power was 350 W. The average recovery in soil samples was 70.39%-85.30%, and the CV was 0.50%-9.99%. The MDLs were below 1 mg/kg. These results using the aforementioned methods met the standards of residue analysis. The present study can provide scientific analysis methods and a basis for evaluation of the study of IL residues in environmental samples.

Evaluating subchronic toxicity of fluoxastrobin using earthworms (Eisenia fetida)

Potential toxicity to soil organisms by fluoxastrobin, a new strobilurin-type fungicide has drawn increasing attention. Thus, the present study investigated the subchronic toxicity induced by exposure to several concentrations (0, 0.1, 1.0, and 2.5 mg kg-1) of fluoxastrobin to earthworms on days 7, 14, 21, and 28. Biochemical indicators (e.g., reactive oxygen species (ROS) content, activities of antioxidase and detoxifying enzymes (superoxide dismutase, catalase, and glutathione S-transferase), lipid peroxidation (malonaldehyde) and degree of DNA damage) were measured. No earthworm deaths were observed during the entire experimental period. For ROS and malonaldehyde, the bioassay values of the three doses reached a maximum on day 21 and then decreased. For superoxide dismutase and glutathione S-transferase, the values increased with the exposure doses of 0.1 and 1.0 mg kg-1 and then decreased. In contrast, the values for catalase were lower on days 7, 14, and 28 and greater on day 21 compared to those of the controls. In addition, the comet assay was more sensitive than other biomarkers, and the degree of DNA damage was dose and time -dependent.

Evaluating toxicity of 1-octyl-3-methylimidazolium hexafluorophosphate to microorganisms in soil

Ionic liquids (ILs) were widely applied because of their excellent properties. The present investigation studied the toxicity of the IL 1-octyl-3-methylimidazolium hexafluorophosphate ([Omim]PF6) to the soil microbial population and community diversity with dose (1.0, 2.0, 4.0, 6.0, and 8.0 mg kg-1) and exposure time (7, 10, and 13 d). The results show the IL was stable during the entire experimental period. The Biolog-ECO plate results indicated that the average well color development (AWCD) in the 6.0 and 8.0 mg kg-1 treatments was lower than these in the other treatments. The diversity indices of the Biolog analysis were significantly reduced. The abundance of the ammonia-oxidizing archaea (AOA-) and the ammonia-oxidizing bacteria (AOB-) ammonia monooxygenase (amoA) genes was measured by the real-time polymerase chain reaction (RT-PCR). In the treatments of 4.0, 6.0 and 8.0 mg kg-1, the abundance of amoA genes of the AOA- and AOB- were inhibited by IL [Omim]PF6.

Influence of isolated bacterial strains on the in situ biodegradation of endosulfan and the reduction of endosulfan- contaminated soil toxicity

The recently discovered endosulfan-degrading bacterial strains Pusillimonas sp. JW2 and Bordetella petrii NS were isolated from endosulfan-polluted water and soil environments. The optimal conditions for the growth and biodegradation activity of the strains JW2 and NS were studied in detail. In addition, the ability of the strains JW2 and NS to biodegrade endosulfan in soils during in situ bioremediation experiments was investigated. At a concentration of 2 mg of endosulfan per kilogram of soil, both JW2 and NS had positive effects on the degradation of endosulfan; JW2 degraded 100% and 91.5% of α- and β-endosulfan, respectively, and NS degraded 95.1% and 90.3% of α- and β-endosulfan, respectively. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of soil samples showed the successful colonization of JW2 and NS, and the toxicity of the soil decreased, as determined by single-cell gel electrophoresis (SCGE) assays of Eiseniafetida and micronucleus (MN) assays of Viciafaba root tip cells. Furthermore, the metabolic products of the bacterially degraded endosulfan from the in situ experiments were identified as endosulfan ether and lactone. This study provided potentially foundational backgrounds information for the remediation of endosulfan-contaminated soil.