Zofia Antoszkiewicz

Effect of oat by-product antioxidants and vitamin E on the oxidative stability of pork from pigs fed diets supplemented with linseed oil

The aim of the experiment was to compare the antioxidative potential of an oat by-product with the effect of vitamin E on the oxidative stability of pork from pigs fed a diet enriched with linseed oil. Thirty-four crossbreed barrows were fed individually from 39 to 109 kg body weight (BW) on one of four diets: a control diet based on barley–triticale–soybean (Diet C), a diet containing an oat by-product (Diet O), and the same diets supplemented with vitamin E (100 mg/kg diet) (Diets CE and OE, respectively). The oat by-product, comprising oat hulls and bran, was included at 10 and 20% in the grower and finisher diets, respectively. To Diets O and OE, refined rapeseed oil was added to equalise their energy content to Diets C and CE. Compared to Diets C and CE, the inclusion of the oat by-product in Diets O and OE increased the antioxidative capacity of water-soluble and lipid soluble compounds in these diets. Dietary treatment did not influence growth performance, slaughter value, longissimus dorsi (LD) muscle quality measured by nutrient contents, pH, drip loss or colour. Vitamin E supplementation increased the α-tocopherol concentration in serum and meat (p < 0.01), and decreased the formation of thiobarbituric acid reactive substances (TBARS) in the fresh and stored LD (p < 0.01). In addition, diets with the oat by-product increased serum α-tocopherol concentration (p < 0.01) and decreased the TBARS levels in the fresh and stored LD (p < 0.05), without increasing muscle α-tocopherol concentration. The obtained results indicate that the phenolic compounds present in oat by-products have a considerable antioxidant potential and a beneficial effect on the pig organism and oxidative stability of meat. However, dietary inclusion with the oat by-product was not as efficient as supplementation with vitamin E.

The Fatty Acid Profile and Oxidative Stability of Meat from Turkeys Fed Diets Enriched with n-3 Polyunsaturated Fatty Acids and Dried Fruit Pomaces as a Source of Polyphenols

The aim of this study was to determine the efficacy of different dietary fruit pomaces in reducing lipid oxidation in the meat of turkeys fed diets with a high content of n-3 polyunsaturated fatty acids (PUFAs). Over a period of 4 weeks before slaughter, turkeys were fed diets with the addition of 5% dried apple, blackcurrant, strawberry and seedless strawberry pomaces (groups AP, BP, SP and SSP, respectively) and 2.5% linseed oil. Pomaces differed in the content (from 5.5 in AP to 43.1 mg/g in SSP) and composition of polyphenols Proanthocyanidins were the main polyphenolic fraction in all pomaces, AP contained flavone glycosides and dihydrochalcones, BP contained anthocyanins, and SP and SSP—ellagitannins. The n-6/n-3 PUFA ratio in all diets was comparable and lower than 2:1. In comparison with groups C and AP, the percentage of n-3 PUFAs in the total fatty acid pool of white meat from the breast muscles of turkeys in groups BP, SP and SSP was significantly higher, proportionally to the higher content of α-linolenic acid in berry pomaces. The fatty acid profile of dark meat from thigh muscles, including the n-6/n-3 PUFA ratio, was similar and lower than 3:1 in all groups. Vitamin A levels in raw breast muscles were higher in group AP than in groups C and BP (P<0.05). The addition of fruit pomaces to turkey diets lowered vitamin E concentrations (P = 0.001) in raw breast muscles relative to group C. Diets supplemented with fruit pomaces significantly lowered the concentration of thiobarbituric acid reactive substances (TBARS) in raw, frozen and cooked meat. Our results indicate that the dietary application of dried fruit pomaces increases the oxidative stability of meat from turkeys fed linseed oil, and strawberry pomace exerted the most desirable effects due to its highest polyphenol content and antioxidant potential.

The effect of dietary vitamin E and selenium supplements on the fatty acid profile and quality traits of eggs

Abstract. The present study investigated the effect of supplementing a diet containing 5 % soybean oil, which provided 30 g/kg of polyunsaturated fatty acids (PUFAs), with two levels of vitamin E (30 or 60 mg/kg, as-is basis) in combination with two levels of selenium (0.15 or 0.30 mg/kg, as-is basis) on hen performance, the fatty acid profile and antioxidant status of the yolk. A 12-week study was performed on 32 Lohmann Brown hens with 8 birds in each group.

Polyphenols in Monogastric Nutrition – A Review

Abstract The popularity of plant-based feed additives in livestock production has increased significantly in the last decade. Polyphenols are secondary plant metabolites which contain bioactive components and deliver positive effects for humans and animals. They are renowned for their anti-inflammatory, immunomodulatory and anti-mutagenic effects. Polyphenols have antioxidant properties, and they minimize the negative consequences of oxidative stress. Their antioxidant capacity is comparable to that of the major biological antioxidants: vitamins E and C. Despite those advantages, polyphenols are characterized by low bioavailability, and further research is needed to harness their full potential in livestock farming. This article presents a review of findings from recent studies investigating the efficacy of polyphenols in monogastric nutrition, with special emphasis on their antioxidant properties.

Antioxidant status of blood and liver of turkeys fed diets enriched with polyunsaturated fatty acids and fruit pomaces as a source of polyphenols.

It was hypothesized that dietary polyphenol-rich fruit pomaces can improve the antioxidant status of both diets and the tissues of turkeys fed such diets. Turkeys were fed diets containing a cellulose preparation (C) or 5% dried apple pomace (AP), blackcurrant pomace (BCP), strawberry pomace (SP) and seedless strawberry pomace (SSP). Blood and liver biochemical parameters were determined in 7 birds from each experimental group slaughtered at 15 weeks of age, after 5 weeks of feeding diets containing soybean oil and linseed oil (approx. 1:1 ratio). Dietary linseed oil added to diets at 2.5% lowered the n-6/n-3 PUFA ratio from approx. 7:1 to below 2:1, thus reducing the antioxidant properties of diets measured using DPPH, ABTS and photo-chemiluminescence assays, compared with diets containing only soybean oil and administered to birds in the first phase of feeding. Fruit pomaces, in particular SSP with the highest polyphenol content (32.81 g/kg) and the highest antioxidant activity (256.4 μM Trolox/g), increased the antioxidant capacity of turkey diets. In comparison with the control group, the dietary treatments with fruit pomaces improved blood antioxidant parameters, including catalase activity (groups AP and BCP), the total antioxidant capacity of hydrophilic (group AP) and lipophilic (groups AP, SP, and SSP) compounds, peroxide levels (groups AP and SSP) and antioxidant capacity measured by the FRAP (ferric reducing antioxidant power of plasma) assay (groups AP, BCP and SSP). Significantly lower concentrations of both vitamin E and thiobarbituric acid reactive substances (TBARS) were noted in the livers of turkeys fed all diets with dried fruit pomaces.

Effect of xylanase supplementation to cereal-based diets on apparent fecal digestibility and growth performance of pigs.

Abstract A total of 300 growing-finishing pigs (30-110 kg BW), the offspring of Naima sows and Duroc boars, were allocated to three dietary treatments (five pens per treatment, 10 males and 10 females per pen). The growth performance of pigs was determined, and the apparent digestibility of nutrients and energy in animals fed grower diets was calculated by the simple balance method. Complete diets with a high content of triticale (70%-73%) and barley (6.8%-12%) were supplemented with endo-1,4-beta-xylanase (6200 EPU per g). The minimum xylanase activity per kg feed was 1050 and 1500 EPU. Xylanase, at activity levels of 1050 and 1500 EPU kg-1 complete diet, significantly (P≤0.05) increased the apparent digestibility of total protein and highly significantly (P≤0.01) that of crude fat. No significant differences in the digestibility of N-free extractives and energy were found between groups. The increased activity of xylanase (1500 EPU kg-1 feed) contributed to an increase in the digestibility of dry matter, crude fibre (P≤0.05) and organic matter (P≤0.01), compared with the control group. Higher (P≤0.01) daily gains and average final body weights were noted in pigs fed diets supplemented with the mono-enzyme preparation. The feed conversion ratio (FCR) tended to improve in pigs receiving xylanase-supplemented diets, but the observed differences were statistically non-significant. Streszczenie Obserwacją objęto 300 rosnących tuczników (30 kg-110 kg) pochodzących z kojarzenia loch linii Naima z knurami rasy Duroc, które podzielono na 3 grupy żywieniowe (5 kojców w grupie, 10 loszek i 10 wieprzków w kojcu). Określono wskaźniki tuczu świń oraz pozorną strawność składników pokarmowych i energii mieszanek grower metodą bilansową prostą. Żywienie tuczników zróżnicowano dodatkiem endo - 1.4-β-ksylanazy o aktywności 6200 EPU w 1 g preparatu, który wprowadzono do mieszanek pełnoporcjowych z wysokim udziałem pszenżyta (70-73%) i jęczmienia (6,8-12%). Minimalna aktywność ksylanazy w 1 kg mieszanki odpowiadała 1050 i 1500 EPU. Zastosowanie w żywieniu tuczników ksylanazy o poziomie aktywności 1050 i 1500 EPU kg-1 mieszanki pełnoporcjowej podwyższyło istotnie (P≤0,05) współczynnik strawności pozornej białka ogólnego oraz wysokoistotnie (P≤0,01) tłuszczu surowego. Nie stwierdzono różnic w strawności związków bezazotowych wyciągowych i energii. Podwyższona aktywność enzymatyczna ksylanazy do 1500 EPU kg-1 wpłynęła na wzrost strawności suchej masy, włókna surowego (P≤0,05) i substancji organicznej (P≤0,01) w stosunku do żywienia kontrolnego. Wyższe (P≤0,01) dobowe przyrosty i średnią masę ciała przy zakończeniu doświadczenia osiągnęły tuczniki żywione mieszankami z dodatkiem preparatu monoenzymatycznego. Tendencje do poprawy wskaźnika wykorzystania paszy w wyniku suplementacji żywienia ksylanazą nie znalazły potwierdzenia statystycznego.

Fiber substrates in the nutrition of weaned piglets – a review

Abstract The present review summarizes the results of 37 experiments in which different types and levels (from 0.5 to 29.7%) of fibrous supplements were used in the formulation of diets for weaned piglets. Diets were supplemented with different sources of insoluble dietary fiber (iDF), soluble dietary fiber (sDF), or mixed DF sources. Most of the applied DF sources decreased the ileal and fecal organic matter digestibility, and they often lowered crude protein digestibility. A moderate addition (1.5-8%) of iDF sources increased average daily feed intake (ADFI) and, frequently, average daily gains (ADG). Sources of sDF as well as high inclusion levels of fiber-rich feeds tended to decrease ADFI and ADG. Improved fecal consistency, decreased diarrhea incidence and antibiotic interventions were confirmed in piglets fed diets with added lignocellulose, cooked or raw oat hulls and wheat bran. The dietary inclusion of iDF rather than sDF sources improved gastrointestinal tract (GIT) development, enzyme activity and gut morphology. An increase in the counts of beneficial gut microbiota and the concentrations of short-chain fatty acids was stimulated by diets with addition iDF or sDF sources. Such diets also slowed down proteolytic fermentation which negatively affects the colonic mucosa. Some research findings indicate that iDF sources improve intestinal barrier function. The analyzed experimental data suggest that the addition of 1.5-2% of a lignocellulose preparation, 2% of oat hulls, 4-8% of coarse wheat bran to diets for weaned piglets may be recommended to promote GIT development and health, and to improve growth performance.

Fermentation and Proteolysis During the Ensilage of Wilted and Unwilted Diploid and Tetraploid Red Clover

The effects of the following factors were analyzed in the study: wilting degree, genetic form of red clover (2n – 4n), cultivars within the genetic form: 2n (Krynia, Parada), 4n (Jubilatka, Bona) and DM x form, DM x 2n, DM x 4n. Fermentation and proteolysis during the ensilage of red clover were affected primarily by wilting, whereas genetic factors (genetic form, cultivar) exerted a lesser effect. However, the genetic form of red clover affected the true protein content of silage and the extent of proteolysis during the ensiling process. The effect of the genetic form of red clover on the extent of proteolysis in silage (at similar levels of water-soluble carbohydrates and buffering capacity) suggests that diploid and tetraploid red clover cultivars differ with respect to chemical properties (poliphenol oxidase activity, polyphenol content) affecting proteolysis.

Effect of Yeast Supplements On Selected Health-Promoting Properties of Lamb Meat

Abstract The objective of this study was to determine the effect of yeast preparations on selected properties of meat from suckling lambs. The experiment was conducted on 30 Kamieniec rams divided into three groups: I - control, II and III - experimental. Throughout the 100-day rearing period, the experimental animals were fed diets supplemented with Saccharomyces cerevisiae prebiotics: Inter Yeast S® brewers yeast in group II, and Biolex®-Beta S in group III. Fat content was determined by Soxhlet extraction. The fatty acid composition of intramuscular fat was determined by esterification followed by gas chromatography analysis. The content of vitamins A and E was determined by liquid chromatography. A significant increase in the content of fat, cholesterol, and vitamin E was noted in the meat of lambs of group II in comparison with control. The fat of animals in group II contained more fatty acids: C14:1, C18:2, C22:6 (P≤0.05), and C18:2 n-6 cis9trans11 (P≤0.01), and less C20:4 (P≤0.01). The fat of animals of group III contained more C18:0 and C22:6 fatty acids (P≤0.05), and less C18:0 (P≤0.05). The fat in this group had also lower concentrations of MUFA (P≤0.05), UFA, and, consequently, a lower UFA/SFA ratio (P≤0.05) in comparison with controls. The yeast-based supplements significantly affected selected health-promoting properties of lamb meat. The applied diet supplements had no significant effect on AI and TI values.