A. Susenbeth

Grassland responses to grazing: effects of grazing intensity and management system in an Inner Mongolian steppe ecosystem

The major aims of this study were, firstly, to analyse the grazing-induced steppe degradation process and, secondly, to identify an efficient and sustainable grazing management system for the widely degraded Inner Mongolian typical steppe ecosystem. From 2005–2008 a grazing experiment was conducted to compare two grazing management systems, the Mixed System (MS) and the Traditional System (TS), along a gradient of seven grazing intensities, i.e. ungrazed (GI0), very-light (GI1), light (GI2), light-moderate (GI3), moderate (GI4), heavy (GI5), and very-heavy (GI6). Each grazing intensity treatment was considered a production unit comprising two adjacent plots, one for hay-making (single-cut system) and one for grazing. Hay-making and grazing alternated annually in the MS, while in the TS the same plots were used either for hay-making or for grazing. Effects of management system, grazing intensity, and year on end-of-season standing biomass (ESSB), aboveground net primary production (ANPP), relative difference in ANPP between 2005 and 2008 (ANPPDiff), relative growth rate (RGR), and sward characteristics (litter accumulation, soil coverage) were analysed. Litter accumulation of production units was affected by grazing intensity (P < 0.001) and decreased from GI0 to GI6 by 83%. Correspondingly, soil coverage decreased (P < 0.001) from GI0 to GI6 by 43%, indicating an increased vulnerability to soil erosion. We found varying compensatory growth responses to grazing intensity among years, probably because of temporal variability in precipitation. The ability of plants to partially compensate for grazing damage was enhanced in years of greater seasonal precipitation. The ANPP of production units was negatively affected by grazing intensity and decreased from GI0 to GI6 by 37, 30, and 55% in 2006 (P < 0.01), 2007 (P < 0.05), and 2008 (P < 0.001), respectively. The effect of management system × grazing intensity interaction on ANPP (P < 0.05) and ANPPDiff (P < 0.05) suggested greater grazing resilience of the MS as compared to the TS at GI3 to GI6.

Short-term management and stocking rate effects of grazing sheep on herbage quality and productivity of Inner Mongolia steppe

Degradation and decreasing productivity increasingly demand sustainable grazing management practices within Inner Mongolian steppe ecosystems. This study focuses on grazing-induced degradation processes over a wide range of stocking rates and aims to identify short-term sensitive indicators and alternative management practices. Short- term effects of 2 grazing management systems (Mixed System and Traditional System) and 7 stocking rates (SR0, SR1.5, SR3, SR4.5, SR6, SR7.5, and SR9 for 0,1.5, 3, 4.5, 6, 7.5, and 9 sheep/ha, respectively) on yielding performance and herbage quality were measured on experimental plots in which moveable exclosures were used on areas chronically grazed by sheep. The experiment was conducted in a typical steppe ecosystem in Inner Mongolia, P. R. China. Results are presented for 2005 and 2006. Sampling time was the main factor affecting yield and quality. Stocking rate also showed considerable effects on yield. HerbagemassdecreasedlinearlyfromSR0toSR9,by85%and82%in2005and2006,respectively.Herbageaccumulation was also affected by stocking rate, and was highest at SR1.5 and clearly reduced at SR9. Grazing effects on relative growth rate indicated grazing tolerance of plants in the short-term, since up to high stocking rates, relative growth rates remained stable. Precipitation also determined plant responses to increasing levels of grazing. The year of higher rainfall generated higher grazing tolerance of plants and higher herbage growth than the drought year. Despite considerable reduction of herbagemass,consistentshort-termresponsesofherbagequalitytograzingin2005and2006werereflectedonlyintermsof crudeproteinandaciddetergentlignin.HerbagecrudeproteincontentwashighestatSR7.5andSR9,whileligninwaslowest at SR7.5 and SR9. Neither productivity nor herbage quality was affected by the management system, suggesting that both systems may be applicable on typical steppe in the short-term.

N balance and cycling of Inner Mongolia typical steppe: a comprehensive case study of grazing effects

Increasing grazing pressure and climate change affect nitrogen (N) dynamics of grassland ecosystems in the Eurasian steppe belt with unclear consequences for future delivery of essential services such as forage production, C sequestration, and diversity conservation. The identification of key processes responsive to grazing is crucial to optimize grassland management. In this comprehensive case study of a Chinese typical steppe, we present an in-depth analysis of grazing effects on N dynamics, including the balance of N gains and losses, and N cycling. N pools and fluxes were simultaneously quantified on three grassland sites of different long-term grazing intensities. Dust deposition, wind erosion, and wet deposition were the predominant but most variable processes contributing to N losses and gains. Heavy grazing increased the risk of N losses by wind erosion. Hay-making and sheep excrement export to folds during nighttime keeping were important pathways of N losses from grassland sites. Compared to the...

Estimating ruminal crude protein degradation with in situ and chemical fractionation procedures

The objective of this study was to utilize the fractionation of feed crude protein (CP) of the Cornell net carbohydrate and protein system (CNCPS) as a basis for estimating undegraded dietary protein (UDP) values of feedstuffs obtained from in situ trials. In addition, the experiments comprised a comparison between in situ UDP values of feedstuffs and CP solubility estimated from the protein dispersibility index. Eleven dairy compound feeds and 21 feedstuffs were inserted in polyester bags and incubated in the rumen of three steers. Values for in situ UDP at assumed ruminal passage rates of 2, 5, and 8% h ˇ1 , respectively, ranged from 63 to 616, 129 to 785, and 167 to 842 g kg ˇ1 of CP. When fish meal data (na2) were excluded from the data set, multiple regression equations that were based on concentrations of CP and cell wall, and on the A, B, and C fractions of the CNCPS fractionation schedule, explained 87, 93, and 94%, respectively, of the variation in UDP values at assumed ruminal passage rates of 2, 5, and 8% h ˇ1 . We conclude that in situ UDP values, which serve as one key variable in many protein evaluation systems for dairy cattle, may be reliably and accurately predicted from chemical fractionation of feed CP according to the CNCPS. The coefficients of determination of estimating UDP values at assumed ruminal passage rates of 2, 5, and 8% h ˇ1 , respectively, from the protein dispersibility index were only 0.30, 0.29, and 0.33. Hence, the protein dispersibility index was not suitable as a predictor of UDP values for the feedstuffs used in the present study. # 2000 Elsevier Science B.V. All rights reserved.

Rotational and continuous grazing of sheep in the Inner Mongolian steppe of China

The aim of this study was to compare the effects of rotational and continuous grazing on herbage mass (HM), organic matter digestibility (dOM) and intake (OMI) and live weight gain (LWG) of sheep grazing on the inner Mongolian steppe, China at a stocking rate of 4.5 sheep/ha during the growing season. In the years 2005 and 2006, four 2-ha plots were used of which two were divided into four 0.5 ha paddocks each for rotational grazing, where sheep were moved each 10 days to the next paddock. The dOM was estimated from faecal crude protein concentration and OMI by oral administration of titanium dioxide. Herbage mass was similar in both grazing systems and dOM and OMI were higher (p < 0.05) at continuous grazing than at rotational grazing, but LWG did not differ probably because of extra energy expenditure for grazing and walking in a larger area. The dOM and OMI decreased (p < 0.05) with progress of the growing season and differed between years. Since precipitation during the growing season in both years was lower than the 30 years average which was probably the reason that positive effects of non-grazing periods on herbage regrowth and quality at rotational grazing could not occur, further studies are required in years with average precipitations before a final evaluation of these grazing systems can be made. Moreover, it seems necessary to quantify energy expenditure for physical activity of animals in grazing studies.

Dietary selection of sheep grazing the semi‐arid grasslands of Inner Mongolia, China at different grazing intensities

The objective of this study was to investigate dietary selection of sheep grazing semi-arid grassland in Inner Mongolia, China, using the difference in organic matter digestibility (OMD) of herbage ingested and herbage on offer as indicator for selection. Faecal N was used as digestibility index for herbage ingested (FOMD), while OMD of herbage on offer (GOMD) was estimated from gas production obtained by the Hohenheim gas test. It was hypothesized that the difference between FOMD and GOMD is high, when grazing animals select against low quality herbage provided that herbage is abundant. In a grazing experiment, six grazing intensities (1.5, 3.0, 4.5, 6.0, 7.5 and 9.0 sheep/ha), representing light to very heavy grazing intensity for the semi-arid grassland, were compared. The amount of herbage on offer decreased with increasing grazing intensity. Independent statistical analysis of FOMD and GOMD showed that the differences between grazing intensities for both OMD determinations (FOMD: 54.0-57.3%, GOMD: 55.2-57.5%) were not significant (p > 0.05). The difference between FOMD and GOMD was not significant for grazing intensities, but varied between sampling periods from -4 to 1 percentage units. In conclusion, the lack of significance for the difference between FOMD and GOMD suggests that for the semi-arid grassland of Inner Mongolia, China, sheep did not select their feed due to a homogeneous nutritional composition of herbage on offer in 2005, regardless of grazing intensity.

Impact of grazing intensity on herbage quality, feed intake and live weight gain of sheep grazing on the steppe of Inner Mongolia*

SUMMARY The grassland steppe of Inner Mongolia is traditionally used for sheep grazing. However, overgrazing reduced vegetation cover in winter, thereby increasing soil erosion and consequently, degradation of the steppe vegetation. Grazing intensity (GI) is still the most important factor in pasture management. Hence, the aim of the current study was to evaluate the effect of GI on grassland and sheep performance. A grazing experiment was conducted from July until September in 2005, 2006 and 2007 in which six different GI ranging from very light (GI 1), light (GI 2), light-moderate (GI 3), moderate (GI 4) and heavy (GI 5) to very heavy (GI 6) were tested. Each GI treatment comprised two adjacent plots that were alternately used for grazing or hay-making each year. Variables measured included herbage mass (HM) and chemical composition, digestibilityof ingested organic matter (dOM), organic matter intake (OMI) and live weight gain (LWG) of sheep. The HM decreased significantly with increasing GI from 1·01 t (GI 1) to 0·45 t dry matter (DM)/ha (GI 6). There were only minor effects of GI on chemical composition and digestibility of standing herbage. Moreover, dOM, OMI and hence, digestible OMI did not differ between GI. Across all study years, LWG of sheep was not influenced by GI so that LWG per hectare increased with increasing GI, reaching a maximum of 730 g/d at GI 6 compared with 181 g/d at GI 1. However, a strong decrease in LWG per sheep with increasing stocking rate was found in 2005 when annual rainfall was less than half of the long-term average, resulting in a similar LWG per hectare across the range of tested stocking rates. The results therefore show that intensive grazing does not reduce growth of individual animals in most years, but increasesLWG per unit of landarea and thus, income of farmers. Thealternatinguse of pastures for grazing or haymaking might have mitigated the negative effects of heavy grazing on herbage and animal performance. Nevertheless, high GI may negatively affect grassland productivity in the long term and the lack of HM on offer on heavy grazed pastures in dry years will require supplement feeding at the end of the vegetation period or the untimely sale of animals.

Effects of rotational and continuous grazing on herbage quality, feed intake and performance of sheep on a semi-arid grassland steppe

Compared to continuous grazing (CG), rotational grazing (RG) increases herbage production and thereby the resilience of grasslands to intensive grazing. Results on feed intake and animal performance, however, are contradictory. Hence, the objective of the study was to determine the effects of RG and CG on herbage mass, digestibility of ingested organic matter (dOM), organic matter intake (OMI) and live weight gain (LWG) of sheep in the Inner Mongolian steppe, China. During June–September 2005–2008, two 2-ha plots were used for each grazing system. In RG, plots were divided into four 0.5-ha paddocks that were grazed for 10 days each at a moderate stocking rate. Instead, CG sheep grazed the whole plots throughout the entire grazing season. At the beginning of every month, dOM was estimated from faecal crude protein concentration. Faeces excretion was determined using titanium dioxide in six sheep per plot. The animals were weighed every month to determine their LWG. Across the years, herbage mass did not differ between systems (p = 0.820). However, dOM, OMI and LWG were lower in RG than in CG (p ≤ 0.005). Thus, our study showed that RG does not improve herbage growth, feed intake and performance of sheep and suggests that stocking rates rather than management system determine the ecological sustainability of pastoral livestock systems in semi-arid environments.

Effect of dietary fibre on nitrogen retention and fibre associated threonine losses in growing pigs

Apart from being an energy source, dietary fibre is also discussed to act as anti-nutritional factor reducing apparent precaecal protein and amino acid (AA) digestibility due to reduced absorption or increased endogenous secretion or both. However, the amounts of protein and AA of endogenous origin determined at the terminal ileum in cannulated animals do not represent the total losses associated with endogenous secretion. A high proportion of secreted protein is reabsorbed and does not reach the terminal ileum, and losses occur during synthesis of endogenous protein. Therefore, the present study used an alternative indirect approach, taking the reduction of nitrogen (N) retention in a threonine (Thr) limited diet as a sensitive indicator for fibre-associated Thr losses. Two experiments were conducted with 12 castrated male pigs each between 37 and 75 kg body weight to measure the effect of the intake of Thr and 150 and 300 g/d fibre from wheat bran (Exp. 1), or 150 g/d fibre from rape seed, cassava leaves, and cassava root peels, respectively (Exp. 2), on N retention. During two (Exp. 1) and three (Exp. 2) balance periods the animals were subjected to the dietary treatments according to a cross-over design. All animals received 1350 g/d of a wheat–soybean-based diet supplemented with free AA to ensure Thr being the first-limiting AA. To determine the effect of Thr on N retention, intake of the basal diet was reduced to 1150 g/d and supplemented with corn starch to reach equal energy intake and an unchanged AA pattern. With increasing BW additional starch was added to all diets to ensure a constant energy intake of 1.25 MJ ME/kg BW0.75. Since the fibre sources contained small amounts of Thr, N retentions were corrected for precaecal digestible Thr intake originating from the fibre sources according to the Thr effect on N retention as determined in experiment 1. Corrected N retentions were affected by fibre level (p = 0.007) and source (p < 0.001). Fibre-associated Thr losses amounted to 3.3, 3.2, 1.2, and 1.1 g/kg fibre from wheat bran, rapeseed, cassava leaf, and cassava root peel, respectively. It is concluded that Thr losses per gram of dietary fibre depend on the fibre source and that fibre concentration and source in pig diets should be considered as a factor affecting Thr requirement.

Effect of dietary Quebracho tannin extract on feed intake, digestibility, excretion of urinary purine derivatives and milk production in dairy cows

ABSTRACT The aim of this study was to evaluate the effects of dietary Quebracho tannin extract (QTE) on feed intake, apparent total tract digestibility (ATTD), excretion of urinary purine derivatives (PD) and milk composition and yield in dairy cows. Fifty Holstein cows were divided into two groups. To reach a similar performance of both groups, cows were divided according to their milk yield, body weight, days in milk and number of lactations at the start of the experiment averaging 33.2 ± 8.2 kg/d, 637 ± 58 kg, 114 ± 73 d and 2.3 ± 1.6 lactations, respectively. The cows were fed a basal diet as total mixed ration containing on dry matter (DM) basis 34% grass silage, 32% maize silage and 34% concentrate feeds. Three dietary treatments were tested, the control (CON, basal diet without QTE), QTE15 (basal diet with QTE at 15 g/kg DM) and QTE30 (basal diet with QTE at 30 g/kg DM). Two treatments were arranged along six periods each 21 d (13 d adaptation phase and 8 d sampling phase). The ATTD of DM and organic matter were reduced only in Diet QTE30, whereas both QTE treatments reduced ATTD of fibre and nitrogen (N), indicating that QTE impaired rumen fermentation. Nevertheless, feed intake was unaffected by QTE. In Diet CON, urinary N excretion accounted for 29.8% of N intake and decreased in treatments QTE15 and QTE30 to 27.5% and 17.9%, respectively. Daily faecal N excretion increased in treatments CON, QTE15 and QTE30 from 211 to 237 and 273 g/d, respectively, which amounted to 39.0%, 42.4% and 51.7% of the N intake, respectively. Hence, QTE shifted N excretion from urine to faeces, whereas the proportion of ingested N appearing in milk was not affected by QTE (average 30.7% of N intake). Daily PD excretion as indicator for microbial crude protein (CP) flow at the duodenum decreased in treatment QTE30 compared with Diet CON from 413 to 280 mmol/d. The ratios of total PD to creatinine suggest that urinary PD excretion was already lower when feeding Diet QTE15. While there was no effect of Diet QTE15, treatment QTE30 reduced milk yield, milk fat and protein. Both QTE treatments reduced milk urea concentration, which suggest that ruminal degradation of dietary CP was reduced. In summary, adding QTE at dosages of 15 and 30 g/kg DM to diets of lactating dairy cows to improve feed and protein use efficiency is not recommended.