Akira Kawarada

Biochemical Studies on “Bakanae” Fungus: Part XXXIV. Isolation of Gibberellins and Their PropertiesPart XXXV.Relation between Gibberellins, A1, A2and Gibberellic Acid

Gibberllin A and B,1,2) the metabolic products of Gibberella fu jikuroi which cause abnormal growth of young tissues of higher plants, were isolated and further investigation to elucidate the chemical structure have been published already3,4, 5,6,7), Gibberellin A, isolated through the process shown in Fig. 1, was chromato gramed on paper using various solvents, butanol-NH3aq, ethanol-NH3aq, benzene acetic acidwater, ethanol-ethyl acetate ligroin, ethanol-ethyl acetate-bezene, but anol saturated with water, but in every case only one spot was found. Further, as the results of the thirty plates count ercurrent distribution method using the solvent system of ethyl. acetate and one mole phosphate buffer adjusted to pH 5.3, we found only one peak. From these

Biochemical Studies on “Bakanae” Fungus. Part 56–57

To confirm the location of the lactone ring at ring A of gihberellin A3, selenium de-hydrogenation experiments were conducted in various ways. The results thus obtained and NMR spectrum studies rather support structure (III).

Biochemical Studies on “Bakanae” Fungus. Part 51–53: Chemical Structure of Gibberellins. Part XVII.Chemical Structure of Gibberellins. Part XVIII.Chemical Structure of Gibberellins. Part XIX.

The outline of this report has already been published in this Journal as a communication to the Editors. The relation among four isomers, gibberellin A1, gibberellin C, pseudogibberellin A1 and isogibberellin A1 was clarified. The partial structure of gibberellin C is presented.

Biochemical Studies on “Bakanae” Fungus. Part 48–50: A New Gibberellin, Gibberellin A4Chemical Structure of Gibberellins. Part XIV Chemical Structure of Gibberellins. Part XV

The outline of this report has already been published in this Journal appearing under “Communication to the Editors” A new gibberellin which was named gibberellin A4 was isolated and characterized.

13. Biochemical Studies on "Bakanae" Fungus:Part 33. 5-Hydroxymethyl-furan-2-carboxylic acid

In the study on the chemical structure of gibberellins, the submerged culture method was applied to obtain a large amount of gibberellins. While isolating them from the medium, a crystal having m.p. 164-5° was obtained and identified to be 5-hydroxy-methyl-2-furan-carboxylic acid.