Yusuke Sumiki

Biochemical Studies on “Bakanae” Fungus: Part XXXIV. Isolation of Gibberellins and Their PropertiesPart XXXV.Relation between Gibberellins, A1, A2and Gibberellic Acid

Gibberllin A and B,1,2) the metabolic products of Gibberella fu jikuroi which cause abnormal growth of young tissues of higher plants, were isolated and further investigation to elucidate the chemical structure have been published already3,4, 5,6,7), Gibberellin A, isolated through the process shown in Fig. 1, was chromato gramed on paper using various solvents, butanol-NH3aq, ethanol-NH3aq, benzene acetic acidwater, ethanol-ethyl acetate ligroin, ethanol-ethyl acetate-bezene, but anol saturated with water, but in every case only one spot was found. Further, as the results of the thirty plates count ercurrent distribution method using the solvent system of ethyl. acetate and one mole phosphate buffer adjusted to pH 5.3, we found only one peak. From these

Biochemical Studies on “Bakanae” Fungus: Part 73. Study on Production and Isolation of Gibberellin A7

The production of gibberellin A7 by Gibberella fujikuroi was studied by using newly devised assay method. Gibberellin A7 increased at preferable temperature range between 32°C and 34°C at the controlled pH(6.0~7.5). The improved isolation process by using column chromatography composed of granular charcoal was found to be extremely convenient, because of its quick elution with satisfactory separation from gibberellic acid which is always accompanied by gibberellin A7 in culture medium.

Studies on the Strengthning of Silkfibres. Part II

Silk fibres were coated by treaitng them with linolenic, linoleic, oleic and palmitic acids in the same method as that reported in Part I of this series, and their properties such as intensity, ductility under dry or wet condition, absorption of moisture and fixation of sericine were examined.