Alessandra Silva Lima

Bovinos submetidos a dietas deficientes em energia por longo período: desempenho animal e sua relação com os teores de T3 e IGF-1

Para avaliar a influencia de dietas deficientes em energia sobre o perfil hormonal, metabolico e clinico em bovinos, foram usados 12 garrotes aleatoriamente distribuidos em tres grupos com quatro animais para receber por 140 dias, as racoes: (G1) adequada, para ganho de peso de 900g/dia (17,7 Mcal/d de ED e 13% de PB); (G2) 80% dos requerimentos de mantenca (5,8 Mcal/d de ED e 7% de PB); e (G3) 60% dessas exigencias (4,7 Mcal/d de ED e 5% de PB). Nos grupos G2 e G3 houve acentuada diminuicao do peso vivo e do consumo de alimentos. O deficit energetico provocou reducao destacada nos teores sanguineos de IGF-1 e T3. A perda de peso foi uma direta consequencia do menor consumo de alimentos, da qualidade inferior de racao ingerida e da menor atuacao de IGF-1 e T3. A mensuracao da altura dos animais ao termino do experimento apresentou uma diferenca no G1 de 59% e 73% maior que G2 e G3, respectivamente. O IGF-1 foi considerado o principal indicador do status energetico, pois diminuiu de maneira sensivel e rapida sua concentracao no decorrer do tempo experimental; este hormonio foi diretamente influenciado pelos teores de T3.

Parenteral administration of vitamins A, D and E on the oxidative metabolism and function of polymorphonuclear leukocytes in swine

ABSTRACT.- Lima A.S., Weigel R.A., Morgado A.A., Nunes G.R., Souza F.N., Moreno A.M., Della Libera A.M.M.P. & Sucupira M.C.A. 2012. Parenteral administration of vitamins A, D and E on the oxidative metabolism and function of polymorphonuclear leukocytes in swine. Pesquisa Veterinaria Brasileira 32(8):727-734. Departamento de Clinica Medica, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo, Av. Prof. Dr. Or-lando M. de Paiva 87, Sao Paulo, SP 05508 270, Brazil. E-mail: msucupir@usp.brThe weaning period of piglets is characterized by physiological alterations, such as de-creased weight gain, increased reactive oxygen species (ROS) and increased serum cortisol levels with possible effects on the immune response. The effect of parenteral administra-tion of vitamins A, D and E on production performance, oxidative metabolism, and the func-tion of polymorphonuclear leukocytes (PMNLs) was assessed in piglets during the weaning period. The sample was comprised of 20 male piglets that were given an injectable ADE vitamin combination (135,000 IU vitamin A, 40,000 IU vitamin D and 40mg vitamin E/animal) at 20 and 40 days of age. Weight gain, concentration of reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD) and the microbicidal and phagocytic activity of PMNLs were assessed. No difference was observed in the average piglet weight during the study; however, a greater percentage of weight gain was observed after weaning in the treated group. The concentrations of GSH and SOD did not differ between groups, although lipid peroxidation was greater in the control group at 60 days of age. The inves-tigated variables of oxidative metabolism were correlated as follows: -0.41 for GSH and MDA, -0.54 for GSH and SOD and 0.34 for MDA and SOD. The intensity of intracellular ROS production, the percentage of ROS-producing PMNLs and the intensity of phagocytosis by PMNLs did not differ between treatment groups. Administration of the injectable ADE com-bination improved the percentage of weight gain between 20 and 40 days of age, decreased oxidative stress at 60 days of age and did not inluence the function of PMNLs in piglets.

Carência alimentar prolongada em garrotes: funções vitais e suas relações com os teores de T3 e IGF-1

ABSTRACT.- Lima A.S., Sucupira M.C.A. & Ortolani E.L. 2014. Long term dietary deficien -cy in steers: vital functions and T 3 and IGF-1 relationships. Pesquisa Veterinaria Brasi-leira 34(9):896-902. Departamento de Clinica Medica, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Sao Paulo, SP 05508 270, Brazil. E-mail: msucupir@usp.brTo evaluate the influence of diets with different degrees of energy deficiency on the hormonal profile and vital functions, 12 steers were randomly distributed into 3 groups of 4 animals. For 140 days, each group received (G1) a diet to promote a weight gain of 900gr/day (17.7 Mcal/d DE and 13% CP), (G2) 80% of the maintenance requirements (5.8 Mcal/d DE and 7% CP), or (G3) 60% of the maintenance requirements (4.7 Mcal/d DE and 5% CP). In G2 and G3, the energy deficit caused a marked decrease in the heart rate and respiratory rate and a reduction in the blood levels of Insulin like growth factor-1 (IGF-1) and triiodo-thyronine (T

Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex

Horses used for the game of polo experience abrupt and frequent changes in exercise intensity. To meet this variable energy demand, the horses use both aerobic and anaerobic pathways in varying proportions and intensities. In this context, there must be a balance between the formation of reactive oxygen species (ROS) and the action of antioxidants to prevent oxidative stress and its consequences. The effect of supplementation with an ADE vitamin complex on oxidative metabolism was evaluated in 18 crossbred horses randomly divided between a treated group (TG) and a control group (CG). The TG animals received the ADE vitamin complex (1mL/50 kg of body weight) by deep intramuscular injection at 30 and 15 days before the game. The CG horses received 10ml of saline by the same administration route and schedule. During the polo match, the animals played for a total of 7.5 min. Blood samples were collected on the same days as the treatments were administered, and immediately before and at 15, 90 and 180 minutes after the game. The concentrations of creatine phosphokinase (CK), lactate dehydrogenase (LDH), lactate, glucose, aspartate aminotransferase (AST), glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in the blood samples. After the game, the TG demonstrated higher levels of AST, lactate and glucose than the CG, suggesting more efficient energy use by the treated animals. The higher GSH and lower lactate levels in the TG before the game suggest the presence of a greater antioxidant supply in the treated animals. The maintenance of the MDA levels indicates that neither of the groups exhibited oxidative stress.

Avaliação do quadro clínico e perfil bioquímico de bovinos durante indução e tratamento de hipocalcemia

The present work aims to study the clinical picture, biochemical profile and treatment response in cattle with induced hypocalcaemia. Were utilized 12 heifers randomly distributed in treated (n = 7) and control (n = 5) groups. The induction model was carried on by continuous EDTA infusion into jugular vein until the animals present clinical signs of hypocalcaemia. After that, the treated group received a calcium (Ca) solution enriched with phosphorus, magnesium and glucose with a dose of 1 mL/kg/BW in 30 minutes, meanwhile, the control group was treated with the same dose of physiologic solution. Clinical examination were performed and blood samples were obtained in times T0 (basal time), T1 (beginning of hypocalcaemia); T2 (end of EDTA infusion); T3 (end of treatment) and T4 (24 hours after the induction). All the heifers present temporary blood calcium and phosphorus reduction and demonstrated classical clinical picture of hypocalcaemia. The treated group present full clinical recovery and blood calcium and phosphorus increase. Most evident clinical signs were increasing heart beat, hypophonesis and rumenal atony. Those symptoms were reversed after calcium treatment. The solution used for treatment was efficient on clinical recovery within thirty minutes, promoting the return to basal levels of the most of biochemical’s variables.