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Dive into the research topics where Alyssa M. Larson is active.

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Featured researches published by Alyssa M. Larson.


Applied Biochemistry and Biotechnology | 2013

Antiviral and antibacterial polyurethanes of various modalities.

Daewon Park; Alyssa M. Larson; Alexander M. Klibanov; Yadong Wang

We have prepared and characterized a new polyurethane-based antimicrobial material, N,N-dodecyl,methyl-polyurethane (Quat-12-PU). It exhibits strong antiviral and antibacterial activities when coated (as an organic solution or an aqueous nanosuspension) onto surfaces and antibacterial activity when electrospun into nanofibers. Quat-12-PU surfaces are able to kill airborne Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria, as well as inactivate the enveloped influenza virus (but not the non-enveloped poliovirus).


Biotechnology and Bioengineering | 2013

Reducing infectivity of HIV upon exposure to surfaces coated with N,N‐dodecyl, methyl‐polyethylenimine

Stephen E. Gerrard; Alyssa M. Larson; Alexander M. Klibanov; Nigel K.H. Slater; Carl V. Hanson; Barbara Abrams; Mary Kate Morris

The infectivity of high-titer, cell-free HIV in culture media and human milk is rapidly reduced upon exposure to polyethylene slides painted with the linear hydrophobic polycation N,N-dodecyl,methyl-polyethylenimine (DMPEI). Accompanying viral p24 protein and free viral RNA analysis of solutions exposed to DMPEI-coated surfaces suggests that virion attachment to the polycationic surface and its subsequent inactivation are the likely mechanism of this phenomenon.


Pharmaceutical Research | 2013

Decreasing Herpes Simplex Viral Infectivity in Solution by Surface-Immobilized and Suspended N,N-Dodecyl, methyl-polyethylenimine

Alyssa M. Larson; Hyung Suk Oh; David M. Knipe; Alexander M. Klibanov

PurposeTo explore surface-immobilized and suspended modalities of the hydrophobic polycation N,N-dodecyl,methyl-polyethylenimine (DMPEI) for the ability to reduce viral infectivity in aqueous solutions containing herpes simplex viruses (HSVs) 1 and 2.MethodsSurface-immobilized (coated onto surfaces) and suspended DMPEI were incubated with aqueous solutions containing HSV-1 or -2 to measure the antiviral effect of the hydrophobic polycation’s formulations on HSVs.ResultsDMPEI coated on either polyethylene slides or male latex condoms dramatically decreases infectivity in solutions containing HSV-1 or -2. Moreover, DMPEI suspended in aqueous solution markedly reduces the infectious titer of these HSVs.ConclusionOur results suggest potential uses of DMPEI for both prophylaxis (in the form of coated condoms) and treatment (as a topical suspension) for HSV infections.


Journal of Pharmaceutical Sciences | 2013

Conjugation to Polymeric Chains of Influenza Drugs Targeting M2 Ion Channels Partially Restores Inhibition of Drug-Resistant Mutants

Alyssa M. Larson; Jianzhu Chen; Alexander M. Klibanov

By attaching multiple copies of the influenza M2 ion channel inhibitors amantadine (1) and rimantadine (2) to polymeric chains, we endeavored to recover their potency in inhibiting drug-resistant influenza viruses. Depending on loading densities, as well as the nature of the drug, the polymer, and the spacer arm, polymer-conjugated drugs were up to 30-fold more potent inhibitors of drug-resistant strains than their monomeric parents. In particular, a 20% loading density and a short linker group on the negatively charged poly-l-glutamate resulted in one of the most potent inhibitors for 2s conjugates against drug-resistant influenza strains. Although full recovery of the inhibitory action against drug-resistant strains was not achieved, this study may be a step toward salvaging anti-influenza drugs that are no longer effective.


Journal of Pharmaceutical Sciences | 2012

Conjugating drug candidates to polymeric chains does not necessarily enhance anti‐influenza activity

Alyssa M. Larson; Hongmei Wang; Yang Cao; Taijiao Jiang; Jianzhu Chen; Alexander M. Klibanov

Using the plaque reduction assay, relatively simple bicyclic quinone molecules, as well as multiple copies thereof covalently attached to a long polyglutamate-based polymeric chain, were examined as new inhibitors of various naturally occurring strains of influenza A virus. The polymer-conjugated inhibitors were found to have a far greater potency (for some as high as two orders of magnitude when a long spacer arm was employed) than their corresponding parent molecules against the human Wuhan influenza strain. However, such polymeric inhibitors failed to exhibit higher potency compared with their small molecule predecessors against the human Puerto Rico and avian turkey influenza strains. These observations, further explored by means of molecular modeling, reveal the previously unrecognized unpredictability of the benefits of multivalency, possibly because of poor accessibility of the viral targets to polymeric agents.


Annual Review of Chemical and Biomolecular Engineering | 2013

Biocidal Packaging for Pharmaceuticals, Foods, and Other Perishables

Alyssa M. Larson; Alexander M. Klibanov


Biotechnology and Bioengineering | 2011

Hydrophobic polycationic coatings disinfect poliovirus and rotavirus solutions.

Alyssa M. Larson; Bryan B. Hsu; Debabrata Rautaray; Jayanta Haldar; Jianzhu Chen; Alexander M. Klibanov


Archive | 2014

Liquid protein formulations containing viscosity-lowering agents

Alyssa M. Larson; Kevin Love; Alisha K. Weight; Alan Crane; Robert S. Langer; Alexander M. Klibanov


Archive | 2014

Liquid protein formulations containing water soluble organic dyes

Alyssa M. Larson; Kevin Love; Alisha K. Weight; Alan Crane; Robert S. Langer; Alexander M. Klibanov


Archive | 2014

Liquid protein formulations containing ionic liquids

Alisha K. Weight; Alyssa M. Larson; Robert S. Langer; Alexander M. Klibanov; Kevin Love; Alan Crane

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Alexander M. Klibanov

Massachusetts Institute of Technology

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Alisha K. Weight

Massachusetts Institute of Technology

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Kevin Love

Massachusetts Institute of Technology

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Jianzhu Chen

Massachusetts Institute of Technology

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Daewon Park

University of Colorado Denver

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Yadong Wang

University of Pittsburgh

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Barbara Abrams

University of California

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