Alyssa M. Larson
Massachusetts Institute of Technology
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Publication
Featured researches published by Alyssa M. Larson.
Applied Biochemistry and Biotechnology | 2013
Daewon Park; Alyssa M. Larson; Alexander M. Klibanov; Yadong Wang
We have prepared and characterized a new polyurethane-based antimicrobial material, N,N-dodecyl,methyl-polyurethane (Quat-12-PU). It exhibits strong antiviral and antibacterial activities when coated (as an organic solution or an aqueous nanosuspension) onto surfaces and antibacterial activity when electrospun into nanofibers. Quat-12-PU surfaces are able to kill airborne Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria, as well as inactivate the enveloped influenza virus (but not the non-enveloped poliovirus).
Biotechnology and Bioengineering | 2013
Stephen E. Gerrard; Alyssa M. Larson; Alexander M. Klibanov; Nigel K.H. Slater; Carl V. Hanson; Barbara Abrams; Mary Kate Morris
The infectivity of high-titer, cell-free HIV in culture media and human milk is rapidly reduced upon exposure to polyethylene slides painted with the linear hydrophobic polycation N,N-dodecyl,methyl-polyethylenimine (DMPEI). Accompanying viral p24 protein and free viral RNA analysis of solutions exposed to DMPEI-coated surfaces suggests that virion attachment to the polycationic surface and its subsequent inactivation are the likely mechanism of this phenomenon.
Pharmaceutical Research | 2013
Alyssa M. Larson; Hyung Suk Oh; David M. Knipe; Alexander M. Klibanov
PurposeTo explore surface-immobilized and suspended modalities of the hydrophobic polycation N,N-dodecyl,methyl-polyethylenimine (DMPEI) for the ability to reduce viral infectivity in aqueous solutions containing herpes simplex viruses (HSVs) 1 and 2.MethodsSurface-immobilized (coated onto surfaces) and suspended DMPEI were incubated with aqueous solutions containing HSV-1 or -2 to measure the antiviral effect of the hydrophobic polycation’s formulations on HSVs.ResultsDMPEI coated on either polyethylene slides or male latex condoms dramatically decreases infectivity in solutions containing HSV-1 or -2. Moreover, DMPEI suspended in aqueous solution markedly reduces the infectious titer of these HSVs.ConclusionOur results suggest potential uses of DMPEI for both prophylaxis (in the form of coated condoms) and treatment (as a topical suspension) for HSV infections.
Journal of Pharmaceutical Sciences | 2013
Alyssa M. Larson; Jianzhu Chen; Alexander M. Klibanov
By attaching multiple copies of the influenza M2 ion channel inhibitors amantadine (1) and rimantadine (2) to polymeric chains, we endeavored to recover their potency in inhibiting drug-resistant influenza viruses. Depending on loading densities, as well as the nature of the drug, the polymer, and the spacer arm, polymer-conjugated drugs were up to 30-fold more potent inhibitors of drug-resistant strains than their monomeric parents. In particular, a 20% loading density and a short linker group on the negatively charged poly-l-glutamate resulted in one of the most potent inhibitors for 2s conjugates against drug-resistant influenza strains. Although full recovery of the inhibitory action against drug-resistant strains was not achieved, this study may be a step toward salvaging anti-influenza drugs that are no longer effective.
Journal of Pharmaceutical Sciences | 2012
Alyssa M. Larson; Hongmei Wang; Yang Cao; Taijiao Jiang; Jianzhu Chen; Alexander M. Klibanov
Using the plaque reduction assay, relatively simple bicyclic quinone molecules, as well as multiple copies thereof covalently attached to a long polyglutamate-based polymeric chain, were examined as new inhibitors of various naturally occurring strains of influenza A virus. The polymer-conjugated inhibitors were found to have a far greater potency (for some as high as two orders of magnitude when a long spacer arm was employed) than their corresponding parent molecules against the human Wuhan influenza strain. However, such polymeric inhibitors failed to exhibit higher potency compared with their small molecule predecessors against the human Puerto Rico and avian turkey influenza strains. These observations, further explored by means of molecular modeling, reveal the previously unrecognized unpredictability of the benefits of multivalency, possibly because of poor accessibility of the viral targets to polymeric agents.
Annual Review of Chemical and Biomolecular Engineering | 2013
Alyssa M. Larson; Alexander M. Klibanov
Biotechnology and Bioengineering | 2011
Alyssa M. Larson; Bryan B. Hsu; Debabrata Rautaray; Jayanta Haldar; Jianzhu Chen; Alexander M. Klibanov
Archive | 2014
Alyssa M. Larson; Kevin Love; Alisha K. Weight; Alan Crane; Robert S. Langer; Alexander M. Klibanov
Archive | 2014
Alyssa M. Larson; Kevin Love; Alisha K. Weight; Alan Crane; Robert S. Langer; Alexander M. Klibanov
Archive | 2014
Alisha K. Weight; Alyssa M. Larson; Robert S. Langer; Alexander M. Klibanov; Kevin Love; Alan Crane