Ángela María Serna López
University of Antioquia
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Publication
Featured researches published by Ángela María Serna López.
Medical Mycology | 2012
Orville Hernández; Agostinho J. Almeida; Diana Tamayo; Isaura Torres; Ana Maria Garcia; Ángela María Serna López; Angela Restrepo; Juan G. McEwen
Adherence of the dimorphic pathogenic fungus Paracoccidioides brasiliensis to lung epithelial cells is considered an essential event for the establishment of infection. We have previously shown that the PbHAD32 hydrolase is important in this early stage of the host-P. brasiliensis yeast cells interaction. The aim of this study was to further elucidate the role of PbHAD32 in conidial thermodimorphism and their interaction with lung epithelial cells. Analysis of the PbHAD32 gene expression revealed higher mRNA levels during the conidia to mycelia (C-M) germination when compared to the conidia to yeast (C-Y) transition. Moreover, PbHAD32 was consistently expressed at higher levels upon infection of lung epithelial cells, but to a greater extent when conidia germinated to produce mycelia. Interestingly, at this particular transitional stage, more conidia adhered to epithelial cells than when they were transiting to the yeast form. Altogether our data further corroborates the importance of PbHAD32 during initial adherence to host cells and suggest that the 32-KDa hydrolase may also participate at different stages of the C-M and C-Y conversions.
PLOS Neglected Tropical Diseases | 2016
Diana Tamayo; Jose F. Muñoz; Ángela María Serna López; Martha Urán; Juan Herrera; Clayton Luiz Borges; Angela Restrepo; Célia Maria de Almeida Soares; Carlos P. Taborda; Agostinho J. Almeida; Juan G. McEwen; Orville Hernández
The ability of Paracoccidioides to defend itself against reactive oxygen species (ROS) produced by host effector cells is a prerequisite to survive. To counteract these radicals, Paracoccidioides expresses, among different antioxidant enzymes, superoxide dismutases (SODs). In this study, we identified six SODs isoforms encoded by the Paracoccidioides genome. We determined gene expression levels of representative isolates of the phylogenetic lineages of Paracoccidioides spp. (S1, PS2, PS3 and Pb01-like) using quantitative RT-PCR. Assays were carried out to analyze SOD gene expression of yeast cells, mycelia cells, the mycelia-to-yeast transition and the yeast-to-mycelia germination, as well as under treatment with oxidative agents and during interaction with phagocytic cells. We observed an increased expression of PbSOD1 and PbSOD3 during the transition process, exposure to oxidative agents and interaction with phagocytic cells, suggesting that these proteins could assist in combating the superoxide radicals generated during the host-pathogen interaction. Using PbSOD1 and PbSOD3 knockdown strains we showed these genes are involved in the response of the fungus against host effector cells, particularly the oxidative stress response, and in a mouse model of infection. Protein sequence analysis together with functional analysis of knockdown strains seem to suggest that PbSOD3 expression is linked with a pronounced extracellular activity while PbSOD1 seems more related to intracellular requirements of the fungus. Altogether, our data suggests that P. brasiliensis actively responds to the radicals generated endogenously during metabolism and counteracts the oxidative burst of immune cells by inducing the expression of SOD isoforms.
Virulence | 2016
Caroline Maria Marcos; Julhiany de Fátima da Silva; Haroldo Cesar de Oliveira; Patricia Akemi Assato; Junya de Lacorte Singulani; Ángela María Serna López; Diana Tamayo; Orville Hernandez-Ruiz; Juan G. McEwen; Maria José Soares Mendes-Giannini; Ana Marisa Fusco-Almeida
The interaction between the fungal pathogen Paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. One molecule implicated in the P. brasiliensis-host interaction is the 14-3-3 protein. The 14-3-3 protein belongs to a family of conserved regulatory molecules that are expressed in all eukaryotic cells and are involved in diverse cellular functions. Here, we investigated the relevance of the 14-3-3 protein to the virulence of P. brasiliensis. Using antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated a 14-3-3-silenced strain (expression reduced by ˜55%). This strain allowed us to investigate the interaction between 14-3-3 and the host and to correlate the functions of P. brasiliensis 14-3-3 with cellular features, such as morphological characteristics and virulence, that are important for pathogenesis.
Nutricion Hospitalaria | 2013
Adriana Giraldo Villa; Ángela María Serna López; Karina Gregoria Mustiola Calleja; Lina Marcela López Gómez; Jorge Hernando Donado Gómez; Juan Manuel Toro Escobar
Íkala | 2011
Cristina Frodden; Ángela María Serna López
Annals of Oncology | 2017
N. Stjepanovic; S. Castro; N. Gadea; Esther Carrasco; M. Codina; Ángela María Serna López; M.E. Semidey; I.M. De Torres; M. Alsina; M. Urioste; L. Pena; F. Mercadillo; Stefania Landolfi; J. Balmana
Medicina U.P.B | 2014
Ángela María Serna López; Jorge Hernando Donado Gómez; Adriana Giraldo Villa; Karina Gregoria Mustiola Calleja; Lina Marcela López Gómez; Camilo Pérez Mejía; Juan Manuel Toro Escobar
Archive | 2010
Ariel Tarazona; Ángela María Serna López; Martha Olivera-Angel
Acta biológica colombiana | 2010
Ariel Tarazona; Ángela María Serna López; Martha Olivera-Angel
Acta Biológica Colombiana | 2010
Ariel Tarazona; Ángela María Serna López; Martha Olivera-Angel