Anne Reifel-Miller
Eli Lilly and Company
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Anne Reifel-Miller.
Journal of Cellular Physiology | 2007
Julie S. Moyers; Tatiyana L. Shiyanova; Farrokh Mehrbod; James D. Dunbar; Timothy Wayne Noblitt; Keith A. Otto; Anne Reifel-Miller; Alexei Kharitonenkov
Fibroblast growth factor (FGF)‐21 is a novel regulator of insulin‐independent glucose transport in 3T3‐L1 adipocytes and has glucose and triglyceride lowering effects in rodent models of diabetes. The precise mechanisms whereby FGF‐21 regulates metabolism remain to be determined. Here we describe the early signaling events triggered by FGF‐21 treatment of 3T3‐L1 adipocytes and reveal a functional interplay between FGF‐21 and peroxisome proliferator‐activated receptor gamma (PPARγ) pathways that leads to a marked stimulation of glucose transport. While the early actions of FGF‐21 on 3T3‐L1 adipocytes involve rapid accumulation of intracellular calcium and phosphorylation of Akt, GSK‐3, p70S6K, SHP‐2, MEK1/2, and Stat3, continuous treatment for 72 h induces an increase in PPARγ protein expression. Moreover, chronic activation of the PPARγ pathway in 3T3‐L1 adipocytes with the PPARγ agonist and anti‐diabetic agent, rosiglitazone (BRL 49653), enhances FGF‐21 action to induce tyrosine phosphorylation of FGF receptor‐2. Strikingly, treatment of cells with FGF‐21 and rosiglitazone in combination leads to a pronounced increase in expression of the GLUT1 glucose transporter and a marked synergy in stimulation of glucose transport. Together these results reveal a novel synergy between two regulators of glucose homeostasis, FGF‐21 and PPARγ, and further define FGF‐21 mechanism of action. J. Cell. Physiol. 210: 1–6, 2007.
Bioorganic & Medicinal Chemistry Letters | 2003
D.L. Gernert; R. Ajamie; R.A. Ardecky; Michael Gregory Bell; Mark D. Leibowitz; Dale A. Mais; Christopher M. Mapes; P.Y. Michellys; Deepa Rungta; Anne Reifel-Miller; John S. Tyhonas; Nathan Yumibe; Timothy Alan Grese
Fluorinated trienoic acid analogues of the RXR selective modulator 1 (LG101506) were synthesized, and tested for their ability to bind RXRalpha and activate RXR homo and heterodimers. Potency and efficacy were observed to be dependent upon the position of fluorination, and improvement in pharmacological profile was demonstrated in some cases.
Nature Genetics | 1999
Chi-Hse Teng; Ann Nestorowicz; Anne Reifel-Miller
We are developing sets of mouse cDNAs with low redundancy for use in microarray analysis. The sets are based on sequence information from GenBank and the Washington University/Merck EST sequencing project. Related sequences from the non-redundant GenBank database were compared with mouse EST clones from the IMAGE consortium. A single EST for a given cluster was selected, colony purified and sequence validated. To evaluate this set of cDNAs for its use in differential gene expression studies, we generated cDNA microarrays. Those microarray slides were used in hybridization experiments using samples of the melanocyte-specific lineage of the neural crest as a model system. Gene expression was compared between RNA samples obtained from mouse melanoma tissue samples of a transgenic hepatocyte growth factor/scatter factor melanoma mouse model and a MEF-derived mouse fibroblast cell line (NIH3T3). Applying a confidence level of 99%, 8.5% of the arrayed genes were found to be differentially expressed. The melanocyte lineage marker genes Dct, Pmel17 and Sox10 are overexpressed in the melanoma samples with a ratio of 7.6, 6.9 and 7.8, respectively. A low expression ratio of 0.6 is seen for the fibroblast marker gene fibronectin. Genes encoding ubiquitin and the heat shock protein Hsc73 display no significant expression changes between the two differential samples.
Journal of Biological Chemistry | 2004
Heidi R. Kast-Woelbern; Sharon L. Dana; Rosemary Cesario; Li Sun; Louise Y. de Grandpre; Mason E. Brooks; Deborah L. Osburn; Anne Reifel-Miller; Kay Klausing; Mark D. Leibowitz
Endocrinology | 2007
Yanfang Liu; M. Dodson Michael; Shera Kash; William R. Bensch; Brett P. Monia; Susan F. Murray; Keith A. Otto; Samreen K. Syed; Sanjay Bhanot; Kyle W. Sloop; John M. Sullivan; Anne Reifel-Miller
Biochemical Journal | 1999
Sharon P. Cousin; Sigrun R. Hügl; Martin G. Myers; Morris F. White; Anne Reifel-Miller; Christopher J. Rhodes
Molecular Endocrinology | 2005
Anne Reifel-Miller; Keith A. Otto; Eric Hawkins; Robert J. Barr; William R. Bensch; Chris Bull; Sharon Dana; Kay Klausing; Jose-Alfredo Martin; Ronit Rafaeloff-Phail; Chahrzad Rafizadeh-Montrose; Gary A. Rhodes; Roger L. Robey; Isabel Rojo; Deepa Rungta; David Snyder; Kelly L. Wilbur; Tony Y. Zhang; Richard W. Zink; Alan M. Warshawsky; Joseph T. Brozinick
Endocrinology | 2006
Mark D. Leibowitz; Robert Ardecky; Marcus F. Boehm; Carol L. Broderick; Mark A. Carfagna; Diane L. Crombie; Jennifer D’Arrigo; Garrett J. Etgen; Margaret M. Faul; Timothy Alan Grese; Henry Havel; Nancy I. Hein; Richard A. Heyman; Diane Jolley; Kay Klausing; Sha Liu; Dale E. Mais; Christopher M. Mapes; Keith B. Marschke; Pierre-Yves Michellys; Chahrzad Montrose-Rafizadeh; Kathleen M. Ogilvie; Bernadette Pascual; Deepa Rungta; John S. Tyhonas; Mary S. Urcan; Marilyn Wardlow; Nathan Yumibe; Anne Reifel-Miller
Journal of Medicinal Chemistry | 2003
Michellys Py; Robert Ardecky; Jyun-Hung Chen; Crombie Dl; Etgen Gj; Margaret M. Faul; Faulkner Al; Timothy Alan Grese; Richard A. Heyman; Donald S. Karanewsky; Klausing K; Leibowitz; Sha Liu; Dale A. Mais; Christopher M. Mapes; Keith B. Marschke; Anne Reifel-Miller; Ogilvie Km; Deepa Rungta; Anthony W. Thompson; John S. Tyhonas; Marcus F. Boehm
Journal of Medicinal Chemistry | 2003
Pierre-Yves Michellys; Robert Ardecky; Jyun-Hung Chen; Jennifer D'Arrigo; Timothy Alan Grese; Donald S. Karanewsky; Mark D. Leibowitz; Sha Liu; Dale A. Mais; Christopher M. Mapes; Chahrzad Montrose-Rafizadeh; Ogilvie Km; Anne Reifel-Miller; Deepa Rungta; Anthony W. Thompson; and John S. Tyhonas; Marcus F. Boehm