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Dive into the research topics where Anne Sophie Gamez is active.

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Featured researches published by Anne Sophie Gamez.


Chest | 2015

Supplementing Defect in Club Cell Secretory Protein Attenuates Airway Inflammation in COPD

Anne Sophie Gamez; Delphine Gras; Aurélie Petit; Lucie Knabe; Nicolas Molinari; Isabelle Vachier; Pascal Chanez; Arnaud Bourdin

BACKGROUND Club cell secretory protein (CCSP) is a protective biomarker associated with annual decline in lung function. COPD progression results from an imbalance between injury and repair initially triggered by cigarette smoking. OBJECTIVE We investigated the effect of CCSP as a therapeutic strategy to restore the balance between injury and repair in COPD simultaneously, validating an ex vivo air-liquid interface (ALI) culture of human bronchial epithelial cells. METHODS Endobronchial biopsy specimens (EBBs) were obtained from 13 patients with COPD, eight smokers, and eight control subjects. Morphometric analysis of the initial EBBs was performed. ALI cultures derived from the same EBBs were exposed to cigarette smoke extract (CSE) with or without exogenous recombinant human CCSP (rhCCSP) supplementation. CCSP and IL-8 concentrations were assessed at steady state and after CSE exposure. RESULTS Morphometric analysis of the initial EBBs showed increased cell density but decreased immunostaining of CCSP+ cells in EBBs of patients with COPD (P = .03 vs control subjects). At steady state, lower CCSP (P = .04) and higher IL-8 levels (P < .0001) were found in COPD ALI epithelium. Exogenous rhCCSP supplementation dampened CSE-induced IL-8-release in patients with COPD and returned to levels similar to those of smokers and control subjects (P = .0001). A negative correlation was found between IL-8-release in ALI and CCSP+ cell density in initial biopsy specimens (P = .0073). CONCLUSIONS In vitro, rhCCSP exogenous supplementation can reverse CSE-induced IL-8 release in biopsy specimens from patients with COPD, indicating a potential use of this strategy in vivo.


BMC Pulmonary Medicine | 2017

Epithelial ciliated beating cells essential for ex vivo ALI culture growth

Delphine Gras; Aurélie Petit; Jérémy Charriot; Lucie Knabe; Khuder Alagha; Anne Sophie Gamez; Céline Garulli; Arnaud Bourdin; Pascal Chanez; Nicolas Molinari; Isabelle Vachier

BackgroundBronchial epithelium plays a key role in orchestrating innate and adaptive immunity. The fate of ex vivo airway epithelial cultures growing at the air liquid interface (ALI) derived from human endobronchial biopsies or brushings is not easy to predict. Calibrating and differentiating these cells is a long and expensive process requiring rigorous expertise. Pinpointing factors associated with ALI culture success would help researchers gain further insight into epithelial progenitor behavior.MethodsA successful ALI culture was defined as one in which a pseudostratified epithelium has formed after 28 days in the presence of all differentiated epithelial cell types. A 4-year prospective bi-center study was conducted with adult subjects enrolled in different approved research protocols.Results463 consecutive endobronchial biopsies were obtained from normal healthy volunteers, healthy smokers, asthmatic patients and smokers with COPD. All demographic variables, the different fiber optic centers and culture operators, numbers of endo-bronchial biopsies and the presence of ciliated cells were carefully recorded. Univariate and multivariate models were developed. A stepwise procedure was used to select the final logistic regression model. ALI culture success was independently associated with the presence of living ciliated cells within the initial biopsy (OR = 2.18 [1.50–3.16], p < 0.001).ConclusionThis finding highlights the properties of the cells derived from the epithelium dedifferentiation process. The preferential selection of samples with ciliated beating cells would probably save time and money. It is still unknown whether successful ALI culture is related to indicators of general cell viability or a purported stem cell state specifically associated with ciliated beating cells.


BioMed Research International | 2013

Distal Airway Impairment in Obese Normoreactive Women

Grégory Marin; Anne Sophie Gamez; Nicolas Molinari; Djamila Kacimi; Isabelle Vachier; Fabrice Paganin; Pascal Chanez; Arnaud Bourdin

Background. Asthma-like symptoms are frequent in overweight and obesity, but the mechanism is unclear when airway hyperresponsiveness (AHR) is lacking. In this study, we focused on obese women with a clinical suspicion of asthma but negative methacholine challenge and tested distal airway hyperreactivity, explored by Forced Vital Capacity dose-response slope (FVC DRS). Objective. To question AHR at the distal airway level in obese women. Methods. A total of 293 symptomatic obese and nonobese women free of treatment were investigated. Methacholine challenge tests were undertaken, and patients were divided according to their results to the test. In hyperreactive and nonhyperreactive patients and in our total population, correlations, regression analyses, and analyses of covariance were performed to compare distal airway hyperreactivity in three groups of body mass index (BMI). Results. After adjusting for age and baseline respiratory values, the relationship between FVC and FEV1 (forced expiratory volume in one second) DRS was influenced by BMI, with a lower slope in obese than overweight and normal patients in our total population (P = 0.008) and in our nonhyperreactive one (P = 0.028). Conclusion. Distal airway hyperresponsiveness was observed in symptomatic wheezing obese women negative to methacholine challenge.


European Respiratory Journal | 2018

Safety and efficacy of pirfenidone in patients carrying telomerase complex mutation

Aurélien Justet; Gabriel Thabut; Effrosyni D. Manali; Maria Molina Molina; Caroline Kannengiesser; Jacques Cadranel; Vincent Cottin; Anne Gondouin; Hilario Nunes; Eline Magois; Cécile Tromeur; Grégoire Prévot; Spyros Papiris; S. Marchand-Adam; Anne Sophie Gamez; Martine Reynaud-Gaubert; Lidwine Wemeau; Bruno Crestani; Raphael Borie

The most frequent mutations in familial pulmonary fibrosis (FPF) involve genes of the telomerase complex such as TERT, TERC, RTEL1, PARN or DKC1 [1]. Mutations within TERT and TERC are found in 15–20% of FPF and are associated with blood, liver and skin disorders. Idiopathic pulmonary fibrosis (IPF) is the most frequent multidisciplinary diagnosis in TERT and TERC mutation carriers [1, 2]. Treatment of IPF in patients who carry a TERT or TERC mutation has not been previously defined. Recently, danazol, a synthetic androgen, was shown to increase telomere length, and seemed to stabilise forced vital capacity (FVC) and diffusing capacity of the lung for carbon monoxide (DLCO) in telomerase complex mutation carriers [3]. Pirfenidone has been shown to reduce FVC decline and to improve progression-free survival in IPF [4, 5]; however, pirfenidone has not been specifically evaluated in telomerase complex mutation carriers. The aim of this multicentre retrospective study was to evaluate the safety and efficacy of pirfenidone in patient carriers of TERT/TERC mutations. In this retrospective study, a beneficial effect of pirfenidone on lung function decline could not be demonstrated in patients carrying TERT/TERC mutation http://ow.ly/VYpJ30i5wSr


The Journal of Allergy and Clinical Immunology | 2017

Club cell secretory protein serum concentration is a surrogate marker of small-airway involvement in asthmatic patients

Sébastien Bommart; Grégory Marin; Nicolas Molinari; Lucie Knabe; Aurélie Petit; Pascal Chanez; Anne Sophie Gamez; Catherine Devautour; Isabelle Vachier; Arnaud Bourdin

Poor asthma control and recurrent exacerbations have been shown to be a phenotypic counterpart of asthma with predominantly small-airway involvement.1 Biomarkers are not always accurate in asthmatic patients, especially in serum, because compartmentalization can occur between the blood and airways. Blood eosinophil counts do not represent an overall view of airway inflammation, and exhaled nitric oxide measurements at different flow rates (fraction of exhaled nitric oxide [Feno] and alveolar nitric oxide [Calvno]) have been developed and validated to reflect more accurately proximal and distal airway inflammation.2 Club cell secretory protein (CCSP) serum concentration has been shown to be associated with chronic obstructive pulmonary disease, bronchiolitis obliterans syndrome, and sarcoidosis, which are all predominantly diseases involving the small airways. Ranges of CCSP concentrations in healthy subjects, reproducibility, and relationships between serum and airway levels are known and can be used as potential surrogate markers. Our aim was to assess small-airway disease in asthmatic patients and to find a related biomarker. We used a dynamic assessment of gas trapping using computed tomographic (CT) imaging of the chest during methacholine challenge as a marker of small-airway disease.


BMC Pulmonary Medicine | 2015

Is generalization of exhaled CO assessment in primary care helpful for early diagnosis of COPD

Nicolas Molinari; Mathieu Abou-Badra; Grégory Marin; Chin-Long Ky; Noemi Amador; Anne Sophie Gamez; Isabelle Vachier; Arnaud Bourdin


Archive | 2017

Asthma: treatment and prevention of pulmonary exacerbations

Jérémy Charriot; Mathilde Volpato; Carey Sueh; Clément Boissin; Anne Sophie Gamez; Isabelle Vachier; Laurence Halimi; Pascal Chanez; Arnaud Bourdin


Revue Des Maladies Respiratoires | 2018

Efficacité du reslizumab chez les patients présentant un asthme éosinophilique avec intolérance à l’aspirine et/ou une rhinosinusite chronique et polypose nasale

Arnaud Bourdin; Anne Sophie Gamez; O. Ledanois; Pascal Chanez


Revue Des Maladies Respiratoires | 2018

Impact du reslizumab sur le recours aux soins chez les patients adultes atteints d’asthme éosinophilique sévère

Arnaud Bourdin; Anne Sophie Gamez; O. Ledanois; Pascal Chanez


Revue Des Maladies Respiratoires | 2017

Efficacité et tolérance de la pirfénidone chez les patients porteurs d’une mutation du complexe télomérase

Aurélien Justet; E. Bergot; Vincent Cottin; Jacques Cadranel; I. Frachon; Anne Sophie Gamez; Anne Gondouin; C. Kanengiesser; Eline Magois; Effrosyni D. Manali; S. Marchand-Adam; M. Molina Molina; A. Mehdaoui; J.-M. Naccache; N. Hilario; Grégoire Prévot; Gabriel Thabut; D. Valeyre; L. Wemeau; Bruno Crestani; Raphael Borie

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Arnaud Bourdin

University of Montpellier

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Pascal Chanez

Aix-Marseille University

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Grégory Marin

University of Montpellier

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Laurence Halimi

University of Montpellier

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Lucie Knabe

University of Montpellier

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Delphine Gras

Aix-Marseille University

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Eline Magois

University of Picardie Jules Verne

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