Arie Srihardyastutie

Optimization Of Elevating Blood Uric Acid Levels With High Purine Diet

Exploration of the use of medicinal plants to lower uric acid levels has been widely practiced. Stages of new drug development research is a preclinical test using experimental animals, therefore the manufacture of an animal model of hyperuricemia is necessary. This study aims to determine the optimal induction of uric acid to increase blood uric acid levels by administering high purine foods such as cow’s liver, cow’s spleen, Gnetum gnemon , emping and fried peanuts. Eighty male white rats were used individuals to be divided into 4 groups, they were: (I) cow’s liver, (II) cow’s liver and cow’s spleen, (III) cow’s liver, cow’s spleen and boiled gnetum gnemon beans, and (IV) cow’s liver, cow’s spleen, emping and fried peanuts. This study using easy touch GCU to measure blood uric acid level. The result of statistical analysis of uric acid level means with 5 times repetition using One Way ANOVA showed that there was a very significant difference between treatments ( p <0,01). The results concluded that high purine diet in group I, II and III had not been able to increase uric acid levels significantly. High purine diet group IV was able to increase blood uric acid levels significantly to make the rats experiencing hyperuricemia with the level of 6.54 mg/dL on day 7 and 13.79 mg/dL on day 14.

Influence of Treating Essential Oils of Canarium Tree Resin on The Level Malondialdehyde (MDA), Protease Activity, Bronchi Histopathology Description and Expression of Tumor Necrosis Alpha Factor (TNF-α) on Asthma Rats (Rattus norvegicus)

Essential oils of canarium tree resin ( Canarium indicum L ) have anti-inflammatory and antioxidant activity. Inflammation of the bronchi and airway remodeling is a major factor in the occurrence of asthma. The purpose of this study was to study the potential of essential oils of canarium tree resin on the expression of TNF-α, changes in bronchial epithelial cell features, MDA levels and protease activity from OVA and LPS exposure in a rat model of asthma. This study used four groups of rat, the negative control group, asthma group, therapy group dose 25 mg/kg BB and 50 mg/kg BB. TNF-α expression was calculated using Walter calculations, histopathologic observation of bronchi was observed using Olympus BX51 microscope, MDA level was measured using Thiobarbituric Acid (TBA) method and measurement of protease activity was done by spectrophotometric method, and analyzed by ANOVA. The results showed that the therapy of essential oils of canarium tree resin at 25 mg/kg BB and 50 mg/kg BB significantly (P<0.05), after treatment for seven days decreased, MDA 44%, Protease activity 46%, improved bronchial epithelium and TNF-α expression by 51%. This study proves that essential oils of canarium tree resin can be effectively used as therapy in asthma model rats.

Effects of Ruellia tuberosa L. Root Extract on the Pancreatics of Diabetic Rat

Diabetes mellitus type 1 (DMT1) is an insulin deficiency disorder caused by pancreatic damage characterized by chronic hyperglycemia. Animal modeling of DMT1 using inducting Multiple Low Dose Streptozotocin (MLD-STZ) elevat levels of free radicals and inflammation in pancreatic organs which then damage β cells. Ruellia tuberosa L. is a plant containing triterpenoids from the class of antioxidant and anti-inflammatory flavonoids. This study used three groups of rat: negative control group, positive control and 240 mg/kg bw therapy group. Study are based on changes MDA levels, blood glucose and histologic of pancreas. MDA level determining with TBA method, glucose levels using EasyTouch GCU and histopalogy of pancreas using Hematoxylin-Eosin staining. Statistical analysis of MDA and blood glucose (α = 0.05) showed significant decreases in MDA and blood glucose levels. Based on the results of histologic observations of pancreas descriptively showed a decreas in cell damage on Langerhans island. Thus, the provision of root extract of Ruellia tuberosa L. can inhibit the damage in rat DMT1 model so that it can be used as therapy of diabetes mellitus.