Chanif Mahdi

Optimization Of Elevating Blood Uric Acid Levels With High Purine Diet

Exploration of the use of medicinal plants to lower uric acid levels has been widely practiced. Stages of new drug development research is a preclinical test using experimental animals, therefore the manufacture of an animal model of hyperuricemia is necessary. This study aims to determine the optimal induction of uric acid to increase blood uric acid levels by administering high purine foods such as cow’s liver, cow’s spleen, Gnetum gnemon , emping and fried peanuts. Eighty male white rats were used individuals to be divided into 4 groups, they were: (I) cow’s liver, (II) cow’s liver and cow’s spleen, (III) cow’s liver, cow’s spleen and boiled gnetum gnemon beans, and (IV) cow’s liver, cow’s spleen, emping and fried peanuts. This study using easy touch GCU to measure blood uric acid level. The result of statistical analysis of uric acid level means with 5 times repetition using One Way ANOVA showed that there was a very significant difference between treatments ( p <0,01). The results concluded that high purine diet in group I, II and III had not been able to increase uric acid levels significantly. High purine diet group IV was able to increase blood uric acid levels significantly to make the rats experiencing hyperuricemia with the level of 6.54 mg/dL on day 7 and 13.79 mg/dL on day 14.

Potential of Lactobacillus casei Shirota Strain as Probiotic toward MDA Levels and Histopathology of Aortic Tissues in Rat with High Cholesterol Diet

Hypercholesterolemia is a major risk factor of cardiovascular disease. Hypercholesterolemia is a condition that the cholesterol levels in the body exceed the normal limits. The main factor that causes high cholesterol levels in the body is a high-cholesterol diet. The provision of highcholesterol diet may improve cholesterol levels, especially LDL cholesterol. This condition leads to LDL oxidation, furthermore generation of free radicals or ROS that causing lipid peroxidation. Products of lipid peroxidation by ROS compound is an MDA compound. Countermeasures against ROS required antioxidant compounds. Probiotics that contain lactic acid bacteria can increase antioxidant. Probiotic of Lactobacillus casei Shirota strain contained of lactic acid bacteria that can increase the antioxidant content and decrease cholesterol levels. The aim of this study was to determine the probiotic of Lactobacillus casei Shirota strain potential to MDA levels of rats blood serum and histopathology of rats aortic tissue that fed with high-cholesterol diet. This study used male white rats (Rattus norvegicus) Wistar strain were divided into negative and positive group and therapy groups doses of Lactobacillus casei Shirota strain is: 0.5, 1 and 1.5 mL/rat/day, that each group contained 5 rats.. The positive control group and therapy group administrated high-cholesterol diet for 21 days. The results showed that probiotic of Lactobacillus casei Shirota strain decreased the MDA levels of blood serum up to 60.15% at dose of 1.5 mL/rat/day and can improve the histopathology of aortic tissue on high-cholesterol diet rats. This result demonstrated that Lactobacillus casei Shirota strain probiotic had potentially as treatment of hypercholesterolemia.

MDA and Histologic Profile of Pancreatic Diabetic-Rats Model Administered With Extract of Glycine max (L.) Merr.

Diabetes Mellitus is characterized by leveling up glucose in human blood and affects increasing of free radicals in body as well as leading to cellular oxidative stress. Experimentally, this condition is able to be characterized by increasing malondialdehyde (MDA) level in cell and histological changing in pancreas appearance. Consumption of antioxidant substances was reported able to reduce the MDA quantity as free radicals. Black soybean or Glycine max (L) Merr. was reported contains important antioxidant agents such as anthocyanin and isoflavone. This paper discloses recent investigation on application of black soybean water extract to reduce the MDA level on diabetes mellitus-rat model induced by STZ (DM) and also reports the pancreas histological changing of the DM rats. Investigation revealed that black soybean water extract significantly affect decreasing of MDA level by 4.9%, 27.1% and 45.7% in three different doses theraphy (500, 750, and 1000 mg/kg BW). Histologically, it also clearly indicates repairing of pancreas tissue of the DM rats.